##gff-version 3
##sequence-region BBa_K199175 1 1893
BBa_K199175	.	engineered_region	1	741	.	+	0	ID=BBa_E5500;Name=BBa_E5500
BBa_K199175	.	ribosome_entry_site	1	15	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E5500
BBa_K199175	.	engineered_region	1	15	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K199175	.	ribosome_entry_site	8	12	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K199175	.	ribosome_entry_site	1	15	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K199175	.	CDS	22	741	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E5500
BBa_K199175	.	CDS	22	741	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K199175	.	engineered_region	750	1893	.	+	0	ID=BBa_K109702;Name=BBa_K109702
BBa_K199175	.	promoter	750	949	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K109702
BBa_K199175	.	non_covalent_binding_site	838	875	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K199175	.	promoter	910	915	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K199175	.	engineered_region	750	949	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K199175	.	CDS	750	837	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K199175	.	non_covalent_binding_site	915	949	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K199175	.	promoter	886	891	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K199175	.	start_codon	922	922	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K199175	.	engineered_region	958	1893	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_K109702
BBa_K199175	.	CDS	976	1731	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K199175	.	point_mutation	1467	1467	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K199175	.	CDS	976	1725	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K199175	.	engineered_region	976	1731	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K199175	.	engineered_tag	1732	1756	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K199175	.	point_mutation	1149	1149	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K199175	.	engineered_region	976	977	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K199175	.	terminator	1765	1844	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K199175	.	engineered_region	1765	1844	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K199175	.	stem_loop	1776	1819	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K199175	.	terminator	1853	1893	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K199175	.	engineered_region	1853	1893	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K199175	.	stop_codon	1886	1886	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K199175	.	stem_loop	1860	1879	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K199175	.	polyA_site	1880	1893	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K199175	.	ribosome_entry_site	958	969	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K199175	.	conserved_region	962	965	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K199175
attaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatg
gtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatt
tatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcg
agatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatat
ttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagtt
aaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatac
atcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaac
tagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacaca
atctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattaca
catggcatggatgaactatacaaataataatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcat
taatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactc
attaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacac
atactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaag
cttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgc
gatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattat
gatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatat
ttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttag
tttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagt
ttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaa
ataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttggga
tatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaaca
aaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgat
agtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttt
atctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata