LOCUS BBa_K199179 2729 bp DNA linear 26-Dec-2024 DEFINITION TT-pLux-RBS-LuxI 2 sites-RBS-RFP-pLacI-RBS-LuxR ACCESSION BBa_K199179 VERSION BBa_K199179.1 FEATURES Location/Qualifiers stem_loop 12..55 /label=stem_loop misc_feature 1..80 /label=BBa_B0010 terminator 1..80 /label=BBa_B0010 stem_loop 96..115 /label=T7 TE misc_feature 122..122 /label=stop polyA_site 116..129 /label=polya misc_feature 89..129 /label=BBa_B0012 terminator 89..129 /label=BBa_B0012 misc_feature 138..157 /label=LuxR/HSL promoter 157..162 /label=-35 promoter 179..184 /label=-10 misc_feature 190..190 /label=start misc_feature 138..192 /label=BBa_R0062 promoter 138..192 /label=BBa_R0062 misc_feature 205..208 /label=conserved RBS 201..212 /label=BBa_B0034 misc_feature 222..229 /label=AsiSI Site misc_feature 235..242 /label=AscI Site CDS 219..845 /label=BBa_K199130 misc_feature 1..845 /label=BBa_S04424 misc_feature 858..861 /label=conserved RBS 854..865 /label=BBa_B0034 CDS 872..1546 /label=mrfp1 misc_feature 1553..1577 /label=Help:Barcodes CDS 872..1577 /label=BBa_E1010 misc_feature 854..1577 /label=BBa_S04419 CDS 1586..1673 /label=end of LacI coding region (inactive) misc_feature 1586..1785 /label=BBa_R0010 misc_feature 1674..1711 /label=CAP binding site promoter 1722..1727 /label=-35 promoter 1746..1751 /label=-10 misc_feature 1751..1785 /label=LacI binding site misc_feature 1758..1758 /label=start promoter 1586..1785 /label=BBa_R0010 misc_feature 1798..1801 /label=conserved RBS 1794..1805 /label=BBa_B0034 misc_feature 1812..1813 /label=prefix misc_feature 1985..1985 /label=T misc_feature 2303..2303 /label=A CDS 1812..2561 /label=luxR misc_feature 1812..2567 /label=BBa_C0062 misc_feature 2568..2592 /label=Help:Barcodes CDS 1812..2567 /label=BBa_C0062 stem_loop 2612..2655 /label=stem_loop misc_feature 2601..2680 /label=BBa_B0010 terminator 2601..2680 /label=BBa_B0010 stem_loop 2696..2715 /label=T7 TE misc_feature 2722..2722 /label=stop polyA_site 2716..2729 /label=polya misc_feature 2689..2729 /label=BBa_B0012 terminator 2689..2729 /label=BBa_B0012 misc_feature 1794..2729 /label=BBa_I0462 misc_feature 1586..2729 /label=BBa_K109702 misc_feature 854..2729 /label=BBa_K199173 ORIGIN 1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 121 gcgtttatat actagagacc tgtaggatcg tacaggttta cgcaagaaaa tggtttgtta 181 tagtcgaata aatactagag aaagaggaga aatactagat ggcgatcgca tctgggcgcg 241 ccgcaattgc aagcggtcgt gcaaccatta tgattaaaaa aagcgatttt ctggccattc 301 cgagcgaaga atataaaggt attctgagcc tgcgctatca ggtttttaaa cagcgtctgg 361 aatgggatct ggtggttgaa aataatctgg aaagtgatga atatgataat agcaatgccg 421 aatatattta tgcctgtgat gataccgaaa atgttagcgg ttgttggcgt ctgctgccga 481 ccaccggtga ttatatgctg aaaagcgttt ttccggaact gctgggtcag cagagcgcac 541 cgaaagatcc gaatattgtt gaactgagcc gttttgccgt gggtaaaaat agcagcaaaa 601 ttaataatag cgccagcgaa attaccatga aactgtttga agccatttat aaacatgccg 661 ttagccaggg tattaccgaa tatgttaccg ttaccagcac cgcaattgaa cgttttctga 721 aacgtattaa agtgccgtgt catcgtattg gcgataaaga aattcatgtt ctgggcgata 781 ccaaaagcgt tgttctgagc atgccgatta atgaacagtt taaaaaagcc gtgctgaact 841 aataatacta gagaaagagg agaaatacta gatggcttcc tccgaagacg ttatcaaaga 901 gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga 961 aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc gctaaactga aagttaccaa 1021 aggtggtccg ctgccgttcg cttgggacat cctgtccccg cagttccagt acggttccaa 1081 agcttacgtt aaacacccgg ctgacatccc ggactacctg aaactgtcct tcccggaagg 1141 tttcaaatgg gaacgtgtta tgaacttcga agacggtggt gttgttaccg ttacccagga 1201 ctcctccctg caagacggtg agttcatcta caaagttaaa ctgcgtggta ccaacttccc 1261 gtccgacggt ccggttatgc agaaaaaaac catgggttgg gaagcttcca ccgaacgtat 1321 gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg 1381 tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc 1441 gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag actacaccat 1501 cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat aacgctgata 1561 gtgctagtgt agatcgctac tagagcaata cgcaaaccgc ctctccccgc gcgttggccg 1621 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac 1681 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg 1741 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacatacta gagaaagagg 1801 agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta 1861 aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac 1921 attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt 1981 caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa 2041 aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat tggaatatat 2101 ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag 2161 gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta 2221 gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga 2281 acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata 2341 ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag 2401 gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc 2461 atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag 2521 caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct 2581 agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg 2641 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc 2701 accttcgggt gggcctttct gcgtttata //