##gff-version 3
##sequence-region BBa_K199179 1 2729
BBa_K199179	.	engineered_region	854	2729	.	+	0	ID=BBa_K199173;Name=BBa_K199173
BBa_K199179	.	engineered_region	854	1577	.	+	0	ID=BBa_S04419;Name=BBa_S04419;Parent=BBa_K199173
BBa_K199179	.	ribosome_entry_site	854	865	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04419
BBa_K199179	.	conserved_region	858	861	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K199179	.	CDS	872	1577	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_S04419
BBa_K199179	.	CDS	872	1546	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K199179	.	engineered_tag	1553	1577	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K199179	.	engineered_region	1586	2729	.	+	0	ID=BBa_K109702;Name=BBa_K109702;Parent=BBa_K199173
BBa_K199179	.	promoter	1586	1785	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K109702
BBa_K199179	.	non_covalent_binding_site	1674	1711	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K199179	.	CDS	1586	1673	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K199179	.	promoter	1746	1751	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K199179	.	non_covalent_binding_site	1751	1785	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K199179	.	promoter	1722	1727	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K199179	.	engineered_region	1586	1785	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K199179	.	start_codon	1758	1758	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K199179	.	engineered_region	1794	2729	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_K109702
BBa_K199179	.	CDS	1812	2567	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K199179	.	engineered_region	1812	1813	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K199179	.	engineered_region	1812	2567	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K199179	.	engineered_tag	2568	2592	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K199179	.	CDS	1812	2561	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K199179	.	point_mutation	1985	1985	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K199179	.	point_mutation	2303	2303	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K199179	.	terminator	2689	2729	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K199179	.	stem_loop	2696	2715	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K199179	.	engineered_region	2689	2729	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K199179	.	polyA_site	2716	2729	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K199179	.	stop_codon	2722	2722	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K199179	.	ribosome_entry_site	1794	1805	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K199179	.	conserved_region	1798	1801	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K199179	.	terminator	2601	2680	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K199179	.	engineered_region	2601	2680	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K199179	.	stem_loop	2612	2655	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K199179	.	engineered_region	1	845	.	+	0	ID=BBa_S04424;Name=BBa_S04424
BBa_K199179	.	terminator	89	129	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S04424
BBa_K199179	.	stem_loop	96	115	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K199179	.	engineered_region	89	129	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K199179	.	polyA_site	116	129	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K199179	.	stop_codon	122	122	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K199179	.	CDS	219	845	.	+	0	ID=BBa_K199130;Name=BBa_K199130;Parent=BBa_S04424
BBa_K199179	.	sequence_feature	222	229	.	+	0	ID=annotation2120504;Name=AsiSI Site;Parent=BBa_K199130
BBa_K199179	.	sequence_feature	235	242	.	+	0	ID=annotation2120505;Name=AscI Site;Parent=BBa_K199130
BBa_K199179	.	terminator	1	80	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S04424
BBa_K199179	.	engineered_region	1	80	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K199179	.	stem_loop	12	55	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K199179	.	promoter	138	192	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_S04424
BBa_K199179	.	promoter	157	162	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K199179	.	engineered_region	138	192	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K199179	.	promoter	179	184	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K199179	.	start_codon	190	190	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K199179	.	non_covalent_binding_site	138	157	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K199179	.	ribosome_entry_site	201	212	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04424
BBa_K199179	.	conserved_region	205	208	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K199179
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtt
tacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatggcgatcgcatctgggc
gcgccgcaattgcaagcggtcgtgcaaccattatgattaaaaaaagcgattttctggccattccgagcgaagaatataa
aggtattctgagcctgcgctatcaggtttttaaacagcgtctggaatgggatctggtggttgaaaataatctggaaagt
gatgaatatgataatagcaatgccgaatatatttatgcctgtgatgataccgaaaatgttagcggttgttggcgtctgc
tgccgaccaccggtgattatatgctgaaaagcgtttttccggaactgctgggtcagcagagcgcaccgaaagatccgaa
tattgttgaactgagccgttttgccgtgggtaaaaatagcagcaaaattaataatagcgccagcgaaattaccatgaaa
ctgtttgaagccatttataaacatgccgttagccagggtattaccgaatatgttaccgttaccagcaccgcaattgaac
gttttctgaaacgtattaaagtgccgtgtcatcgtattggcgataaagaaattcatgttctgggcgataccaaaagcgt
tgttctgagcatgccgattaatgaacagtttaaaaaagccgtgctgaactaataatactagagaaagaggagaaatact
agatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacga
gttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggt
ccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgaca
tcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgt
taccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtcc
gacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctga
aaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaa
aaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatc
gttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctac
tagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactgg
aaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttc
cggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaaa
aacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgct
tatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctga
tatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctata
gtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaa
atgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcgg
aatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccatta
attgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagag
aaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtac
tgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaatttta
acaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggca
tcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactag
agtcacactggctcaccttcgggtgggcctttctgcgtttata