LOCUS BBa_K199180 2746 bp DNA linear 29-Nov-2024 DEFINITION TT-pLux-RBS-LuxI 2 sites-RBS-GFP-pLacI-RBS-LuxR ACCESSION BBa_K199180 VERSION BBa_K199180.1 FEATURES Location/Qualifiers stem_loop 12..55 /label=stem_loop misc_feature 1..80 /label=BBa_B0010 terminator 1..80 /label=BBa_B0010 stem_loop 96..115 /label=T7 TE misc_feature 122..122 /label=stop polyA_site 116..129 /label=polya misc_feature 89..129 /label=BBa_B0012 terminator 89..129 /label=BBa_B0012 misc_feature 138..157 /label=LuxR/HSL promoter 157..162 /label=-35 promoter 179..184 /label=-10 misc_feature 190..190 /label=start misc_feature 138..192 /label=BBa_R0062 promoter 138..192 /label=BBa_R0062 misc_feature 205..208 /label=conserved RBS 201..212 /label=BBa_B0034 misc_feature 222..229 /label=AsiSI Site misc_feature 235..242 /label=AscI Site CDS 219..845 /label=BBa_K199130 misc_feature 1..845 /label=BBa_S04424 RBS 854..868 /label=RBS-1\Strong RBS 861..865 /label=RBS misc_feature 854..868 /label=BBa_B0030 RBS 854..868 /label=BBa_B0030 CDS 875..1594 /label=GFP protein CDS 875..1594 /label=BBa_E0040 misc_feature 854..1594 /label=BBa_E5500 CDS 1603..1690 /label=end of LacI coding region (inactive) misc_feature 1603..1802 /label=BBa_R0010 misc_feature 1691..1728 /label=CAP binding site promoter 1739..1744 /label=-35 promoter 1763..1768 /label=-10 misc_feature 1768..1802 /label=LacI binding site misc_feature 1775..1775 /label=start promoter 1603..1802 /label=BBa_R0010 misc_feature 1815..1818 /label=conserved RBS 1811..1822 /label=BBa_B0034 misc_feature 1829..1830 /label=prefix misc_feature 2002..2002 /label=T misc_feature 2320..2320 /label=A CDS 1829..2578 /label=luxR misc_feature 1829..2584 /label=BBa_C0062 misc_feature 2585..2609 /label=Help:Barcodes CDS 1829..2584 /label=BBa_C0062 stem_loop 2629..2672 /label=stem_loop misc_feature 2618..2697 /label=BBa_B0010 terminator 2618..2697 /label=BBa_B0010 stem_loop 2713..2732 /label=T7 TE misc_feature 2739..2739 /label=stop polyA_site 2733..2746 /label=polya misc_feature 2706..2746 /label=BBa_B0012 terminator 2706..2746 /label=BBa_B0012 misc_feature 1811..2746 /label=BBa_I0462 misc_feature 1603..2746 /label=BBa_K109702 misc_feature 854..2746 /label=BBa_K199175 ORIGIN 1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 121 gcgtttatat actagagacc tgtaggatcg tacaggttta cgcaagaaaa tggtttgtta 181 tagtcgaata aatactagag aaagaggaga aatactagat ggcgatcgca tctgggcgcg 241 ccgcaattgc aagcggtcgt gcaaccatta tgattaaaaa aagcgatttt ctggccattc 301 cgagcgaaga atataaaggt attctgagcc tgcgctatca ggtttttaaa cagcgtctgg 361 aatgggatct ggtggttgaa aataatctgg aaagtgatga atatgataat agcaatgccg 421 aatatattta tgcctgtgat gataccgaaa atgttagcgg ttgttggcgt ctgctgccga 481 ccaccggtga ttatatgctg aaaagcgttt ttccggaact gctgggtcag cagagcgcac 541 cgaaagatcc gaatattgtt gaactgagcc gttttgccgt gggtaaaaat agcagcaaaa 601 ttaataatag cgccagcgaa attaccatga aactgtttga agccatttat aaacatgccg 661 ttagccaggg tattaccgaa tatgttaccg ttaccagcac cgcaattgaa cgttttctga 721 aacgtattaa agtgccgtgt catcgtattg gcgataaaga aattcatgtt ctgggcgata 781 ccaaaagcgt tgttctgagc atgccgatta atgaacagtt taaaaaagcc gtgctgaact 841 aataatacta gagattaaag aggagaaata ctagatgcgt aaaggagaag aacttttcac 901 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt 961 cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac 1021 tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt atggtgttca 1081 atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc 1141 cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg 1201 tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga 1261 ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa 1321 tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca 1381 caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg 1441 cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa 1501 agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat 1561 tacacatggc atggatgaac tatacaaata ataatactag agcaatacgc aaaccgcctc 1621 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag 1681 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt 1741 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca 1801 catactagag aaagaggaga aatactagat gaaaaacata aatgccgacg acacatacag 1861 aataattaat aaaattaaag cttgtagaag caataatgat attaatcaat gcttatctga 1921 tatgactaaa atggtacatt gtgaatatta tttactcgcg atcatttatc ctcattctat 1981 ggttaaatct gatatttcaa tcctagataa ttaccctaaa aaatggaggc aatattatga 2041 tgacgctaat ttaataaaat atgatcctat agtagattat tctaactcca atcattcacc 2101 aattaattgg aatatatttg aaaacaatgc tgtaaataaa aaatctccaa atgtaattaa 2161 agaagcgaaa acatcaggtc ttatcactgg gtttagtttc cctattcata cggctaacaa 2221 tggcttcgga atgcttagtt ttgcacattc agaaaaagac aactatatag atagtttatt 2281 tttacatgcg tgtatgaaca taccattaat tgttccttct ctagttgata attatcgaaa 2341 aataaatata gcaaataata aatcaaacaa cgatttaacc aaaagagaaa aagaatgttt 2401 agcgtgggca tgcgaaggaa aaagctcttg ggatatttca aaaatattag gttgcagtga 2461 gcgtactgtc actttccatt taaccaatgc gcaaatgaaa ctcaatacaa caaaccgctg 2521 ccaaagtatt tctaaagcaa ttttaacagg agcaattgat tgcccatact ttaaaaatta 2581 ataacactga tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag 2641 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac 2701 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata //