##gff-version 3
##sequence-region BBa_K199180 1 2746
BBa_K199180	.	engineered_region	854	2746	.	+	0	ID=BBa_K199175;Name=BBa_K199175
BBa_K199180	.	engineered_region	1603	2746	.	+	0	ID=BBa_K109702;Name=BBa_K109702;Parent=BBa_K199175
BBa_K199180	.	promoter	1603	1802	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K109702
BBa_K199180	.	CDS	1603	1690	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K199180	.	promoter	1763	1768	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K199180	.	non_covalent_binding_site	1768	1802	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K199180	.	promoter	1739	1744	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K199180	.	start_codon	1775	1775	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K199180	.	non_covalent_binding_site	1691	1728	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K199180	.	engineered_region	1603	1802	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K199180	.	engineered_region	1811	2746	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_K109702
BBa_K199180	.	terminator	2706	2746	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K199180	.	stem_loop	2713	2732	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K199180	.	polyA_site	2733	2746	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K199180	.	stop_codon	2739	2739	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K199180	.	engineered_region	2706	2746	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K199180	.	CDS	1829	2584	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K199180	.	engineered_region	1829	2584	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K199180	.	engineered_region	1829	1830	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K199180	.	point_mutation	2002	2002	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K199180	.	engineered_tag	2585	2609	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K199180	.	point_mutation	2320	2320	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K199180	.	CDS	1829	2578	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K199180	.	ribosome_entry_site	1811	1822	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K199180	.	conserved_region	1815	1818	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K199180	.	terminator	2618	2697	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K199180	.	stem_loop	2629	2672	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K199180	.	engineered_region	2618	2697	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K199180	.	engineered_region	854	1594	.	+	0	ID=BBa_E5500;Name=BBa_E5500;Parent=BBa_K199175
BBa_K199180	.	CDS	875	1594	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E5500
BBa_K199180	.	CDS	875	1594	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K199180	.	ribosome_entry_site	854	868	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E5500
BBa_K199180	.	engineered_region	854	868	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K199180	.	ribosome_entry_site	861	865	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K199180	.	ribosome_entry_site	854	868	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K199180	.	engineered_region	1	845	.	+	0	ID=BBa_S04424;Name=BBa_S04424
BBa_K199180	.	ribosome_entry_site	201	212	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04424
BBa_K199180	.	conserved_region	205	208	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K199180	.	promoter	138	192	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_S04424
BBa_K199180	.	engineered_region	138	192	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K199180	.	promoter	157	162	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K199180	.	start_codon	190	190	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K199180	.	promoter	179	184	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K199180	.	non_covalent_binding_site	138	157	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K199180	.	CDS	219	845	.	+	0	ID=BBa_K199130;Name=BBa_K199130;Parent=BBa_S04424
BBa_K199180	.	sequence_feature	235	242	.	+	0	ID=annotation2120505;Name=AscI Site;Parent=BBa_K199130
BBa_K199180	.	sequence_feature	222	229	.	+	0	ID=annotation2120504;Name=AsiSI Site;Parent=BBa_K199130
BBa_K199180	.	terminator	1	80	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S04424
BBa_K199180	.	stem_loop	12	55	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K199180	.	engineered_region	1	80	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K199180	.	terminator	89	129	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S04424
BBa_K199180	.	stem_loop	96	115	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K199180	.	polyA_site	116	129	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K199180	.	stop_codon	122	122	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K199180	.	engineered_region	89	129	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K199180
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtt
tacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatggcgatcgcatctgggc
gcgccgcaattgcaagcggtcgtgcaaccattatgattaaaaaaagcgattttctggccattccgagcgaagaatataa
aggtattctgagcctgcgctatcaggtttttaaacagcgtctggaatgggatctggtggttgaaaataatctggaaagt
gatgaatatgataatagcaatgccgaatatatttatgcctgtgatgataccgaaaatgttagcggttgttggcgtctgc
tgccgaccaccggtgattatatgctgaaaagcgtttttccggaactgctgggtcagcagagcgcaccgaaagatccgaa
tattgttgaactgagccgttttgccgtgggtaaaaatagcagcaaaattaataatagcgccagcgaaattaccatgaaa
ctgtttgaagccatttataaacatgccgttagccagggtattaccgaatatgttaccgttaccagcaccgcaattgaac
gttttctgaaacgtattaaagtgccgtgtcatcgtattggcgataaagaaattcatgttctgggcgataccaaaagcgt
tgttctgagcatgccgattaatgaacagtttaaaaaagccgtgctgaactaataatactagagattaaagaggagaaat
actagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggca
caaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactgga
aaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcata
tgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgg
gaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgatttt
aaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaac
aaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatca
acaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaa
gatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaac
tatacaaataataatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacg
acaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggc
tttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagagg
agaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataa
tgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcat
tctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaa
taaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgt
aaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacg
gctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgt
gtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaa
cgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatatta
ggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagta
tttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatc
actactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg
tgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata