LOCUS       BBa_K199184             1586 bp    DNA     linear       22-Nov-2024
DEFINITION  pT7-RBS-GFP-RBS-RFP-pTet-RBS
ACCESSION   BBa_K199184
VERSION     BBa_K199184.1  
FEATURES             Location/Qualifiers
     misc_feature    18..18
                     /label=Transcription Start Site
     misc_feature    1..11
                     /label=Polymerase Binding Region
     misc_feature    12..23
                     /label=Initiation Region
     promoter        1..23
                     /label=BBa_R0085
     RBS             32..46
                     /label=RBS-1\Strong
     RBS             39..43
                     /label=RBS
     misc_feature    32..46
                     /label=BBa_B0030
     RBS             32..46
                     /label=BBa_B0030
     CDS             53..772
                     /label=GFP protein
     CDS             53..772
                     /label=BBa_E0040
     misc_feature    1..772
                     /label=BBa_K199176
     misc_feature    785..788
                     /label=conserved
     RBS             781..792
                     /label=BBa_B0034
     CDS             799..1473
                     /label=mrfp1
     misc_feature    1480..1504
                     /label=Help:Barcodes
     CDS             799..1504
                     /label=BBa_E1010
     misc_feature    781..1504
                     /label=BBa_S04419
     misc_feature    1513..1531
                     /label=TetR 1
     misc_feature    1513..1566
                     /label=BBa_R0040
     promoter        1532..1537
                     /label=-35
     misc_feature    1538..1556
                     /label=TetR 2
     promoter        1555..1560
                     /label=-10
     promoter        1513..1566
                     /label=BBa_R0040
     misc_feature    1579..1582
                     /label=conserved
     RBS             1575..1586
                     /label=BBa_B0034
     promoter        1513..1586
                     /label=BBa_J13002
     misc_feature    781..1586
                     /label=BBa_S04468
ORIGIN
        1 taatacgact cactataggg agatactaga gattaaagag gagaaatact agatgcgtaa
       61 aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa
      121 tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac
      181 ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac
      241 tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt
      301 tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga
      361 cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat
      421 cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata
      481 caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt
      541 taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag accattatca
      601 acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac
      661 acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt
      721 tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataat aatactagag
      781 aaagaggaga aatactagat ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc
      841 aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa
      901 ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg
      961 ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa
     1021 cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa
     1081 cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa
     1141 gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg
     1201 gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac
     1261 ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac
     1321 gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa
     1381 accgacatca aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac
     1441 gaacgtgctg aaggtcgtca ctccaccggt gcttaataac gctgatagtg ctagtgtaga
     1501 tcgctactag agtccctatc agtgatagag attgacatcc ctatcagtga tagagatact
     1561 gagcactact agagaaagag gagaaa
//