BBa_K199184
type: ComponentDefinition
persistent identity: BBa K199184
display id: BBa_K199184
version: 1
was generated by: iGEM to SBOL conversion
description: pT7-RBS-GFP-RBS-RFP-pTet-RBS
created: 2010-07-18T11:00:00Z
modified: 2015-05-08T01:11:21Z
mutable provenance: BioBricks
owned by: james
owned by: myers
mutable description: pT7+RBS+GFP+RBS+RFP+pTet+RBS
discontinued: false
dominant: false
experience: experience/None
group u list: _295_
m user id: 0
owner id: 5111
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: Eric Sawyer
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_S04468
component: BBa_K199176
sequence annotation: BBa_K199176
sequence annotation: BBa_S04468
sequence: https://synbiohub.org/public/igem/BBa_K199184_sequence/1

BBa_S04468
type: Component
persistent identity: component2268402
display id: component2268402
version: 1
top level: BBa_K199184
definition: BBa_S04468
access: public

BBa_K199176
type: Component
persistent identity: component2268387
display id: component2268387
version: 1
top level: BBa_K199184
definition: BBa_K199176
access: public

BBa_K199176
type: SequenceAnnotation
persistent identity: annotation2268387
display id: annotation2268387
version: 1
top level: BBa_K199184
location: https://synbiohub.org/public/igem/BBa_K199184/annotation2268387/range2268387/1
component: BBa_K199176

https://synbiohub.org/public/igem/BBa_K199184/annotation2268387/range2268387/1
type: Range
persistent identity: range2268387
display id: range2268387
version: 1
top level: BBa_K199184
direction: direction/reverse
start: 1
end: 772
orientation: inline

BBa_S04468
type: SequenceAnnotation
persistent identity: annotation2268402
display id: annotation2268402
version: 1
top level: BBa_K199184
location: https://synbiohub.org/public/igem/BBa_K199184/annotation2268402/range2268402/1
component: BBa_S04468

https://synbiohub.org/public/igem/BBa_K199184/annotation2268402/range2268402/1
type: Range
persistent identity: range2268402
display id: range2268402
version: 1
top level: BBa_K199184
direction: direction/reverse
start: 781
end: 1586
orientation: inline

BBa_S04468
type: ComponentDefinition
persistent identity: BBa S04468
display id: BBa_S04468
version: 1
was generated by: iGEM to SBOL conversion
description: S04419:J13002
created: 2010-07-15T11:00:00Z
modified: 2015-05-08T01:14:40Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 5111
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Eric Sawyer
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J13002
component: BBa_S04419
sequence annotation: BBa_S04419
sequence annotation: BBa_J13002
sequence: https://synbiohub.org/public/igem/BBa_S04468_sequence/1

BBa_J13002
type: Component
persistent identity: component2250248
display id: component2250248
version: 1
top level: BBa_S04468
definition: BBa_J13002
access: public

BBa_S04419
type: Component
persistent identity: component2250240
display id: component2250240
version: 1
top level: BBa_S04468
definition: BBa_S04419
access: public

BBa_S04419
type: SequenceAnnotation
persistent identity: annotation2250240
display id: annotation2250240
version: 1
top level: BBa_S04468
location: https://synbiohub.org/public/igem/BBa_S04468/annotation2250240/range2250240/1
component: BBa_S04419

https://synbiohub.org/public/igem/BBa_S04468/annotation2250240/range2250240/1
type: Range
persistent identity: range2250240
display id: range2250240
version: 1
top level: BBa_S04468
direction: direction/reverse
start: 1
end: 724
orientation: inline

BBa_J13002
type: SequenceAnnotation
persistent identity: annotation2250248
display id: annotation2250248
version: 1
top level: BBa_S04468
location: https://synbiohub.org/public/igem/BBa_S04468/annotation2250248/range2250248/1
component: BBa_J13002

https://synbiohub.org/public/igem/BBa_S04468/annotation2250248/range2250248/1
type: Range
persistent identity: range2250248
display id: range2250248
version: 1
top level: BBa_S04468
direction: direction/reverse
start: 733
end: 806
orientation: inline

BBa_K199176
type: ComponentDefinition
persistent identity: BBa K199176
display id: BBa_K199176
version: 1
was generated by: iGEM to SBOL conversion
description: pT7+RBS+GFP
created: 2010-07-15T11:00:00Z
modified: 2015-05-08T01:11:21Z
mutable provenance: BioBricks
owned by: james
owned by: myers
mutable description: This part expresses GFP in the presence of T7 RNA polymerase.
discontinued: false
dominant: false
group u list: _295_
m user id: 0
owner id: 5111
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: Eric Sawyer
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_E0040
component: BBa_B0030
component: BBa_R0085
sequence annotation: BBa_R0085
sequence annotation: BBa_B0030
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K199176_sequence/1

BBa_E0040
type: Component
persistent identity: component2074004
display id: component2074004
version: 1
top level: BBa_K199176
definition: GFP
access: public

BBa_B0030
type: Component
persistent identity: component2074001
display id: component2074001
version: 1
top level: BBa_K199176
definition: BBa_B0030
access: public

BBa_R0085
type: Component
persistent identity: component2073999
display id: component2073999
version: 1
top level: BBa_K199176
definition: BBa_R0085
access: public

BBa_R0085
type: SequenceAnnotation
persistent identity: annotation2073999
display id: annotation2073999
version: 1
top level: BBa_K199176
location: https://synbiohub.org/public/igem/BBa_K199176/annotation2073999/range2073999/1
component: BBa_R0085

https://synbiohub.org/public/igem/BBa_K199176/annotation2073999/range2073999/1
type: Range
persistent identity: range2073999
display id: range2073999
version: 1
top level: BBa_K199176
direction: direction/reverse
start: 1
end: 23
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2074001
display id: annotation2074001
version: 1
top level: BBa_K199176
location: https://synbiohub.org/public/igem/BBa_K199176/annotation2074001/range2074001/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K199176/annotation2074001/range2074001/1
type: Range
persistent identity: range2074001
display id: range2074001
version: 1
top level: BBa_K199176
direction: direction/forward
start: 32
end: 46
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2074004
display id: annotation2074004
version: 1
top level: BBa_K199176
location: https://synbiohub.org/public/igem/BBa_K199176/annotation2074004/range2074004/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K199176/annotation2074004/range2074004/1
type: Range
persistent identity: range2074004
display id: range2074004
version: 1
top level: BBa_K199176
direction: direction/reverse
start: 53
end: 772
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_R0085
type: ComponentDefinition
persistent identity: BBa R0085
display id: BBa_R0085
version: 1
was generated by: iGEM to SBOL conversion
description: T7 Consensus Promoter Sequence
created: 2005-02-21T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: Sequence obtained from Sri Kosuri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The T7 promoter should only produce PoPS when the T7 polymerase is also being expressed.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Barry Canton
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: Polymerase Binding Region
sequence annotation: Initiation Region
sequence annotation: Transcription Start Site
sequence: https://synbiohub.org/public/igem/BBa_R0085_sequence/1

Polymerase Binding Region
type: SequenceAnnotation
persistent identity: annotation1721521
display id: annotation1721521
version: 1
top level: BBa_R0085
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721521/range1721521/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0085/annotation1721521/range1721521/1
type: Range
persistent identity: range1721521
display id: range1721521
version: 1
top level: BBa_R0085
direction: direction/forward
start: 1
end: 11
orientation: inline

Initiation Region
type: SequenceAnnotation
persistent identity: annotation1721522
display id: annotation1721522
version: 1
top level: BBa_R0085
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721522/range1721522/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_R0085/annotation1721522/range1721522/1
type: Range
persistent identity: range1721522
display id: range1721522
version: 1
top level: BBa_R0085
direction: direction/forward
start: 12
end: 23
orientation: inline

Transcription Start Site
type: SequenceAnnotation
persistent identity: annotation1721520
display id: annotation1721520
version: 1
top level: BBa_R0085
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721520/range1721520/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_R0085/annotation1721520/range1721520/1
type: Range
persistent identity: range1721520
display id: range1721520
version: 1
top level: BBa_R0085
direction: direction/forward
start: 18
end: 18
orientation: inline

BBa_J13002
type: ComponentDefinition
persistent identity: BBa J13002
display id: BBa_J13002
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressed POPS/RIPS generator
created: 2005-06-15T11:00:00Z
modified: 2015-08-31T04:08:29Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: true
experience: experience/Works
group u list: _37_5_
m user id: 0
owner id: 88
owning group id: 37
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Jeff Tabor
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_B0034
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J13002_sequence/1

BBa_B0034
type: Component
persistent identity: component1535786
display id: component1535786
version: 1
top level: BBa_J13002
definition: BBa_B0034
access: public

BBa_R0040
type: Component
persistent identity: component1535778
display id: component1535778
version: 1
top level: BBa_J13002
definition: p(tetR)
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1535778
display id: annotation1535778
version: 1
top level: BBa_J13002
location: https://synbiohub.org/public/igem/BBa_J13002/annotation1535778/range1535778/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J13002/annotation1535778/range1535778/1
type: Range
persistent identity: range1535778
display id: range1535778
version: 1
top level: BBa_J13002
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1535786
display id: annotation1535786
version: 1
top level: BBa_J13002
location: https://synbiohub.org/public/igem/BBa_J13002/annotation1535786/range1535786/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J13002/annotation1535786/range1535786/1
type: Range
persistent identity: range1535786
display id: range1535786
version: 1
top level: BBa_J13002
direction: direction/forward
start: 63
end: 74
orientation: inline

BBa_S04419
type: ComponentDefinition
persistent identity: BBa S04419
display id: BBa_S04419
version: 1
was generated by: iGEM to SBOL conversion
description: B0034:E1010
created: 2010-01-22T12:00:00Z
modified: 2015-05-08T01:14:39Z
owned by: james
owned by: myers
part status: Released HQ 2013
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 5562
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Mary Gearing
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_E1010
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_S04419_sequence/1

BBa_E1010
type: Component
persistent identity: component2244399
display id: component2244399
version: 1
top level: BBa_S04419
definition: mRFP1
access: public

BBa_B0034
type: Component
persistent identity: component2244396
display id: component2244396
version: 1
top level: BBa_S04419
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2244396
display id: annotation2244396
version: 1
top level: BBa_S04419
location: https://synbiohub.org/public/igem/BBa_S04419/annotation2244396/range2244396/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S04419/annotation2244396/range2244396/1
type: Range
persistent identity: range2244396
display id: range2244396
version: 1
top level: BBa_S04419
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2244399
display id: annotation2244399
version: 1
top level: BBa_S04419
location: https://synbiohub.org/public/igem/BBa_S04419/annotation2244399/range2244399/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_S04419/annotation2244399/range2244399/1
type: Range
persistent identity: range2244399
display id: range2244399
version: 1
top level: BBa_S04419
direction: direction/reverse
start: 19
end: 724
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

https://synbiohub.org/public/igem/BBa_K199184_sequence/1
type: Sequence
persistent identity: BBa K199184 sequence
display id: BBa_K199184_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagatactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04468_sequence/1
type: Sequence
persistent identity: BBa S04468 sequence
display id: BBa_S04468_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K199176_sequence/1
type: Sequence
persistent identity: BBa K199176 sequence
display id: BBa_K199176_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagatactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0085_sequence/1
type: Sequence
persistent identity: BBa R0085 sequence
display id: BBa_R0085_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J13002_sequence/1
type: Sequence
persistent identity: BBa J13002 sequence
display id: BBa_J13002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04419_sequence/1
type: Sequence
persistent identity: BBa S04419 sequence
display id: BBa_S04419_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z