BBa_K199016
1
BBa_K199016
CUAGC tRNA Suppressor (Produces Serine)
2009-07-01T11:00:00Z
2015-05-08T01:11:18Z
Oligo assembly
A serine tRNA was modified to have the anticodon loop 5' CUGCUAGAA 3'so that the anticodon GCUAG could be used to decode a frameshift mutation of CUAGC in a mRNA.
false
false
_295_
0
5118
9
It's complicated
false
none
false
Clifton Davis
annotation2007936
1
5 nt anitcodon
range2007936
1
46
50
BBa_K199045
1
BBa_K199045
CUACC tRNA Suppressor (Produces Serine)
2009-07-19T11:00:00Z
2015-05-08T01:11:19Z
Assembly from oligos
A serine tRNA was modified to have the anticodon loop 5' GUGGUAGAA 3'so that the anticodon GGUAG could be used to decode a frameshift mutation of CUACC in a mRNA.
false
false
_295_
0
5115
9
It's complicated
false
NO
false
William Vernon
annotation2011866
1
5nt anti codon
range2011866
1
46
50
BBa_R0010
1
LacI
promoter (lacI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
false
true
_1_
0
24
7
In stock
false
<P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
true
annotation1961223
1
CAP binding site
range1961223
1
89
126
annotation1961222
1
BBa_R0010
range1961222
1
1
200
annotation1961227
1
start
range1961227
1
173
173
annotation1961221
1
end of LacI coding region (inactive)
range1961221
1
1
88
annotation1961225
1
-10
range1961225
1
161
166
annotation1961226
1
LacI binding site
range1961226
1
166
200
annotation1961224
1
-35
range1961224
1
137
142
BBa_K199048
1
BBa_K199048
CCACC tRNA Suppressor (Produces Serine)
2009-07-19T11:00:00Z
2015-05-08T01:11:19Z
Oligo assembly
A serine tRNA was modified to have the anticodon loop 5' UUGGUGGAA 3'so that the anticodon GGUGG could be used to decode a frameshift mutation of CCACC in a mRNA.
false
false
_295_
0
5115
9
It's complicated
false
no
false
William Vernon
annotation2011945
1
5nt anti codon
range2011945
1
46
50
BBa_K199189
1
BBa_K199189
pLac+tRNA CCACC+pLac+tRNA CUAGC+pLac+tRNA CUACC
2010-07-20T11:00:00Z
2015-05-08T01:11:21Z
BioBricks
pLac+tRNA CCACC+pLac+tRNA CUAGC+pLac+tRNA CUACC
false
false
_295_
0
5111
9
It's complicated
false
N/A
false
Eric Sawyer
component2074991
1
BBa_R0010
component2074999
1
BBa_K199045
component2074981
1
BBa_K199048
component2074973
1
BBa_R0010
component2074990
1
BBa_K199016
component2074982
1
BBa_R0010
annotation2074981
1
BBa_K199048
range2074981
1
209
351
annotation2074999
1
BBa_K199045
range2074999
1
927
1069
annotation2074991
1
BBa_R0010
range2074991
1
719
918
annotation2074982
1
BBa_R0010
range2074982
1
360
559
annotation2074990
1
BBa_K199016
range2074990
1
568
710
annotation2074973
1
BBa_R0010
range2074973
1
1
200
BBa_K199045_sequence
1
ggatccaattcggagagatgccggagcggctgaacggaccggtgtggtagaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
BBa_K199189_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagggatccaattcggagagatgccggagcggctgaacggaccggtttggtggaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagcttactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagggatccaattcggagagatgccggagcggctgaacggaccggtctgctagaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagcttactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagggatccaattcggagagatgccggagcggctgaacggaccggtgtggtagaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
BBa_R0010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
BBa_K199016_sequence
1
ggatccaattcggagagatgccggagcggctgaacggaccggtctgctagaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
BBa_K199048_sequence
1
ggatccaattcggagagatgccggagcggctgaacggaccggtttggtggaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z