##gff-version 3
##sequence-region BBa_K199194 1 3478
BBa_K199194	.	engineered_region	854	3478	.	+	0	ID=BBa_S04473;Name=BBa_S04473
BBa_K199194	.	engineered_region	1603	3478	.	+	0	ID=BBa_K199173;Name=BBa_K199173;Parent=BBa_S04473
BBa_K199194	.	engineered_region	2335	3478	.	+	0	ID=BBa_K109702;Name=BBa_K109702;Parent=BBa_K199173
BBa_K199194	.	promoter	2335	2534	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K109702
BBa_K199194	.	non_covalent_binding_site	2500	2534	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K199194	.	non_covalent_binding_site	2423	2460	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K199194	.	engineered_region	2335	2534	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K199194	.	promoter	2471	2476	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K199194	.	CDS	2335	2422	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K199194	.	start_codon	2507	2507	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K199194	.	promoter	2495	2500	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K199194	.	engineered_region	2543	3478	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_K109702
BBa_K199194	.	terminator	3350	3429	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K199194	.	stem_loop	3361	3404	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K199194	.	engineered_region	3350	3429	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K199194	.	ribosome_entry_site	2543	2554	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K199194	.	conserved_region	2547	2550	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K199194	.	CDS	2561	3316	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K199194	.	CDS	2561	3310	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K199194	.	engineered_region	2561	3316	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K199194	.	engineered_tag	3317	3341	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K199194	.	point_mutation	2734	2734	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K199194	.	engineered_region	2561	2562	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K199194	.	point_mutation	3052	3052	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K199194	.	terminator	3438	3478	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K199194	.	engineered_region	3438	3478	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K199194	.	stop_codon	3471	3471	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K199194	.	stem_loop	3445	3464	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K199194	.	polyA_site	3465	3478	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K199194	.	engineered_region	1603	2326	.	+	0	ID=BBa_S04419;Name=BBa_S04419;Parent=BBa_K199173
BBa_K199194	.	CDS	1621	2326	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_S04419
BBa_K199194	.	CDS	1621	2295	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K199194	.	engineered_tag	2302	2326	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K199194	.	ribosome_entry_site	1603	1614	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04419
BBa_K199194	.	conserved_region	1607	1610	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K199194	.	engineered_region	854	1594	.	+	0	ID=BBa_E5500;Name=BBa_E5500;Parent=BBa_S04473
BBa_K199194	.	ribosome_entry_site	854	868	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E5500
BBa_K199194	.	engineered_region	854	868	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K199194	.	ribosome_entry_site	861	865	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K199194	.	ribosome_entry_site	854	868	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K199194	.	CDS	875	1594	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E5500
BBa_K199194	.	CDS	875	1594	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K199194	.	engineered_region	1	845	.	+	0	ID=BBa_S04424;Name=BBa_S04424
BBa_K199194	.	CDS	219	845	.	+	0	ID=BBa_K199130;Name=BBa_K199130;Parent=BBa_S04424
BBa_K199194	.	sequence_feature	235	242	.	+	0	ID=annotation2120505;Name=AscI Site;Parent=BBa_K199130
BBa_K199194	.	sequence_feature	222	229	.	+	0	ID=annotation2120504;Name=AsiSI Site;Parent=BBa_K199130
BBa_K199194	.	ribosome_entry_site	201	212	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04424
BBa_K199194	.	conserved_region	205	208	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K199194	.	terminator	1	80	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S04424
BBa_K199194	.	stem_loop	12	55	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K199194	.	engineered_region	1	80	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K199194	.	promoter	138	192	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_S04424
BBa_K199194	.	non_covalent_binding_site	138	157	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K199194	.	promoter	179	184	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K199194	.	engineered_region	138	192	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K199194	.	start_codon	190	190	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K199194	.	promoter	157	162	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K199194	.	terminator	89	129	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S04424
BBa_K199194	.	engineered_region	89	129	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K199194	.	stop_codon	122	122	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K199194	.	stem_loop	96	115	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K199194	.	polyA_site	116	129	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_K199194
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtt
tacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatggcgatcgcatctgggc
gcgccgcaattgcaagcggtcgtgcaaccattatgattaaaaaaagcgattttctggccattccgagcgaagaatataa
aggtattctgagcctgcgctatcaggtttttaaacagcgtctggaatgggatctggtggttgaaaataatctggaaagt
gatgaatatgataatagcaatgccgaatatatttatgcctgtgatgataccgaaaatgttagcggttgttggcgtctgc
tgccgaccaccggtgattatatgctgaaaagcgtttttccggaactgctgggtcagcagagcgcaccgaaagatccgaa
tattgttgaactgagccgttttgccgtgggtaaaaatagcagcaaaattaataatagcgccagcgaaattaccatgaaa
ctgtttgaagccatttataaacatgccgttagccagggtattaccgaatatgttaccgttaccagcaccgcaattgaac
gttttctgaaacgtattaaagtgccgtgtcatcgtattggcgataaagaaattcatgttctgggcgataccaaaagcgt
tgttctgagcatgccgattaatgaacagtttaaaaaagccgtgctgaactaataatactagagattaaagaggagaaat
actagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggca
caaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactgga
aaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcata
tgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgg
gaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgatttt
aaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaac
aaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatca
acaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaa
gatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaac
tatacaaataataatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgt
ttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaag
gtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttcca
gtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaa
tgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttca
tctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagc
ttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggt
cactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatca
aactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccgg
tgcttaataacgctgatagtgctagtgtagatcgctactagagcaatacgcaaaccgcctctccccgcgcgttggccga
ttcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagct
cactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaattt
cacacatactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaat
taaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattattta
ctcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaat
attatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaa
tatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactggg
tttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatag
atagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatat
agcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctct
tgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaata
caacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataaca
ctgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttc
gttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttta
ta