BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961227 1 start range1961227 1 173 173 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961225 1 -10 range1961225 1 161 166 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961224 1 -35 range1961224 1 137 142 BBa_J04500 1 BBa_J04500 IPTG inducible promoter with RBS 2005-06-08T11:00:00Z 2015-08-31T04:08:14Z Davidson Synth-Aces Released HQ 2013 R0010.B0034 false true _16_ 0 326 16 In stock false false Kristen DeCelle component1508149 1 BBa_R0010 component1508159 1 BBa_B0034 annotation1508149 1 BBa_R0010 range1508149 1 1 200 annotation1508159 1 BBa_B0034 range1508159 1 209 220 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_S04537 1 BBa_S04537 K199199:J61046 2010-12-12T12:00:00Z 2015-05-08T01:14:41Z false false _9_ 0 5562 9 Not in stock false false Mary Gearing component2114992 1 BBa_J61046 component2114991 1 BBa_K199199 annotation2114992 1 BBa_J61046 range2114992 1 267 300 annotation2114991 1 BBa_K199199 range2114991 1 1 258 BBa_K199199 1 BBa_K199199 ATG-loxP-STOP sequence 2010-12-12T12:00:00Z 2015-05-08T01:11:21Z loxP is J61042 Sequence also contains bidirectional transcriptional terminator B0014. The stop sequence prevents transcription and translation. When excised, the downstream reporter genes (K199198 and K199197) are translated in frame. false false _295_ 0 5562 9 Not in stock false Designed to include multiple stop codons in all 6 reading frames. false Mary Gearing BBa_J61046 1 lox [Lox] site for recombination 2007-02-20T12:00:00Z 2015-08-31T02:03:00Z bob Released HQ 2013 bob false false _95_ 0 483 95 In stock false bob true John Anderson BBa_K199200 1 BBa_K199200 pLac-RBS-ATG-loxP-STOP-loxP 2010-12-12T12:00:00Z 2015-05-08T01:11:21Z See individual subparts. When placed upstream of a reporter gene (K199197 or K199198), this sequence prevents expression of the gene. If the STOP sequence is excised, the reporter gene is excised. false false _295_ 0 5562 9 Not in stock false n/a false Mary Gearing component2115004 1 BBa_S04537 component2115001 1 BBa_J04500 annotation2115004 1 BBa_S04537 range2115004 1 227 526 annotation2115001 1 BBa_J04500 range2115001 1 1 220 BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_B0034_sequence 1 aaagaggagaaa BBa_J61046_sequence 1 ataacttcgtataatgtatgctatacgaagttat BBa_J04500_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa BBa_S04537_sequence 1 atgataacttcgtataatgtatgctatacgaagttataaggcctgattatgaagagcgtgtgacagagacacatagaatatctcacacgacatggcagttaaataataaaaaagccggattaataatctggctttttattattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagcgtgaactgccaatctgggttaagttatcttaggttttctgcaccgcgccttctttaatcaggcctttactagagataacttcgtataatgtatgctatacgaagttat BBa_K199200_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgataacttcgtataatgtatgctatacgaagttataaggcctgattatgaagagcgtgtgacagagacacatagaatatctcacacgacatggcagttaaataataaaaaagccggattaataatctggctttttattattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagcgtgaactgccaatctgggttaagttatcttaggttttctgcaccgcgccttctttaatcaggcctttactagagataacttcgtataatgtatgctatacgaagttat BBa_K199199_sequence 1 atgataacttcgtataatgtatgctatacgaagttataaggcctgattatgaagagcgtgtgacagagacacatagaatatctcacacgacatggcagttaaataataaaaaagccggattaataatctggctttttattattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagcgtgaactgccaatctgggttaagttatcttaggttttctgcaccgcgccttctttaatcaggcctt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z