LOCUS       BBa_K1992006            2624 bp    DNA     linear       22-Nov-2024
DEFINITION  Histamin-Tar GFP tagged expression system (promoter+RBS+coding+term
            inator)
ACCESSION   BBa_K1992006
VERSION     BBa_K1992006.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     RBS             44..67
                     /label=Tar native RBS
     RBS             44..67
                     /label=BBa_K1992000
     misc_feature    323..338
                     /label=Region I mutations
     misc_feature    578..608
                     /label=Region II mutations
     CDS             108..670
                     /label=Ligand-Binding domain
     CDS             74..1735
                     /label=BBa_K1992002
     misc_feature    1744..1761
                     /label=BBa_J18921
     CDS             1768..2487
                     /label=GFP protein
     CDS             1768..2487
                     /label=BBa_E0040
     stem_loop       2507..2550
                     /label=stem_loop
     misc_feature    2496..2575
                     /label=BBa_B0010
     terminator      2496..2575
                     /label=BBa_B0010
     stem_loop       2591..2610
                     /label=T7 TE
     misc_feature    2617..2617
                     /label=stop
     polyA_site      2611..2624
                     /label=polya
     misc_feature    2584..2624
                     /label=BBa_B0012
     terminator      2584..2624
                     /label=BBa_B0012
     terminator      2496..2624
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaacttgt tttcaggaag
       61 gtgcctttac tagatgatta accgtatccg cgtagtcacg ctgttggtaa tggtgctggg
      121 ggtattcgca ctgttacagc ttatttccgg cagtctgttt ttttcttccc ttcaccatag
      181 ccagaagagc tttgtggttt ccaatcaatt acgggaacag cagggcgagc tgacgtcaac
      241 ctgggattta atgctgcaaa cggaaattaa cctgtggcgt tcagcggtac ggatgatgat
      301 ggattcctcc aatcaacaaa gtaacgccaa agttgaattg ctcgatagcg ccaggaaaac
      361 attggcgcag gcagcgacgc attataaaaa attcaaaagc atggcaccgt tacctgaaat
      421 ggtcgctacc agtcgtaata ttgatgaaaa atataaaaac tattacacag cgttaactga
      481 actgattgat tacctagatt atggcaatac tggagctgaa gtggctcatc caacctgggg
      541 aatgatgaat gcaatgggcg aagcgtttgc tcagtacgcc ctcagcagtg aaaaactgta
      601 tcgcgatatc gtcactgaca acgcagatga ttaccgattt gcccagtggc aactggcggt
      661 tatcgcgctg gtggtggtat tgattctgct ggtggcgtgg tacggcattc gccgtatgtt
      721 gcttactccg ctggcaaaaa ttattgctca cattcgcgaa atcgccggtg gtaacctggc
      781 gaataccctg accattgacg ggcgcagtga aatgggcgac ctggcgcaga gcgtttcaca
      841 tatgcaacgc tctttgactg acaccgtcac tcatgtccgc gaaggttcag atgccatcta
      901 tgccggtacc cgtgaaattg cggcgggcaa caccgatctt tcctcccgta ctgaacagca
      961 ggcatccgcg ctggaagaaa ctgccgccag catggagcag ctcaccgcga cagtgaagca
     1021 aaacgccgat aacgcccgcc aggcctcgca actggcgcaa agtgcctccg acaccgccca
     1081 gcacggcggc aaagtggtgg atggcgtagt gaaaacgatg catgagatcg ccgatagttc
     1141 gaagaaaatt gccgacatta tcagcgttat cgacggtatt gccttccaga ctaatatcct
     1201 cgcgctgaat gccgcggttg aagccgcgcg tgcgggtgaa cagggccgtg gttttgccgt
     1261 ggtggcgggt gaagtgcgta atcttgccag tcgcagcgcc caggcggcaa aagagatcaa
     1321 agccctcatt gaagactccg tctcacgcgt tgataccggt tcggtgctgg tcgaaagcgc
     1381 cggggaaaca atgaacaata tcgtcaatgc tgtcactcgc gtgactgaca ttatgggcga
     1441 gattgcatcg gcatcggatg aacagagccg tggcatcgat caagtcgcat tggcggtttc
     1501 ggaaatggat cgcgtcacgc aacagaacgc atcgctggtg caggaatcag ctgccgccgc
     1561 cgctgcgctg gaagaacagg cgagtcgttt aacgcaagca gtttccgcgt tccgtctggc
     1621 agccagccca ctcaccaata aaccgcaaac accatcccgt cctgccagtg agcaaccacc
     1681 ggctcagcca cgactgcgaa ttgctgaaca agatccaaac tgggaaacat tttgatacta
     1741 gagggtagcg gcagcggtag ctactagatg cgtaaaggag aagaactttt cactggagtt
     1801 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga
     1861 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga
     1921 aaactacctg ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt
     1981 gcgagatacc cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt
     2041 tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa
     2101 gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa
     2161 gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac
     2221 atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt
     2281 gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
     2341 cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc
     2401 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat
     2461 ggcatggatg aactatacaa ataataatac tagagccagg catcaaataa aacgaaaggc
     2521 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     2581 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//