Histamin-Tar GFP tagged expression system (promoter+RBS+coding+terminator)
rhlR sequence and plasmid was recived form Prop. Roee Amit lab
This part contain rhlR promoter as an induced promoter for the expression of Tar chemoreceptor
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
GFP (mut3b) [note that this part does not have a barcode]
Double terminator consisting of BBa_B0010 and BBa_B0012
Highly flexible 6 amino acid linker. Translates to gsgsgs. Codon-optimize for E. coli, yeast, mammalian.
* gene synthesis * codon optimized for E. coli
The sequence of the desired segments for both the NarX and Tar???s cytoplasmic region were obtained from the complete E coli genome sequence http://www.ecogene.org/sources/1
NarX is a sensor protein which is part of the E. coli anaerobic respiration system and mediates response to nitrite and nitrate.
gBlocks were not compatible so we had to split them into two fragments
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
Conversion of the iGEM parts registry to SBOL2.1