LOCUS       BBa_K1992007            2118 bp    DNA     linear       22-Nov-2024
DEFINITION  PctA-Tar expression system (promoter+RBS+coding+terminator)
ACCESSION   BBa_K1992007
VERSION     BBa_K1992007.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..1123
                     /label=PctA LBD + HAMP
     CDS             1124..1980
                     /label=Tar signaling domain
     misc_feature    160..160
                     /label=c
     misc_feature    463..463
                     /label=a
     misc_feature    466..466
                     /label=a
     misc_feature    1057..1057
                     /label=g
     CDS             62..1981
                     /label=BBa_K1992001
     stem_loop       2001..2044
                     /label=stem_loop
     misc_feature    1990..2069
                     /label=BBa_B0010
     terminator      1990..2069
                     /label=BBa_B0010
     stem_loop       2085..2104
                     /label=T7 TE
     misc_feature    2111..2111
                     /label=stop
     polyA_site      2105..2118
                     /label=polya
     misc_feature    2078..2118
                     /label=BBa_B0012
     terminator      2078..2118
                     /label=BBa_B0012
     terminator      1990..2118
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgatcaaa agtctgaagt tcagccacaa gatcctcctc gcggcctccc tcgtggtgtt
      121 cgccgctttc gcactgttca ccctctacaa cgattacctc cagcgcaacg cgatccgcga
      181 ggacctcgaa agctacctgc gggaaatggg tgacgtgact tccagcaaca ttcagaactg
      241 gctcggcggc cgtctgctgc tggtcgagca gaccgcccag accctggccc gcgaccacag
      301 cccggaaacc gtcagcgccc tgctcgaaca gccggccctg acctcgacct tcagcttcac
      361 ctacctcggc cagcaggacg gcgtcttcac catgcgtccg gacagcccga tgccggccgg
      421 ctacgatccg cgcagccggc cctggtacaa ggacgccgtg gcagcaggcg gcctgaccct
      481 gaccgaaccc tacgtcgacg ccgccaccca ggaattgatc atcaccgcgg cgacaccggt
      541 gaaggccgct ggcaacaccc tcggcgtggt aggcggcgac ctcagcctga agaccctggt
      601 gcagatcatc aattcgctgg acttcagcgg catgggctac gccttcctgg tcagcggcga
      661 cggcaagatc ctggtgcacc cggacaaaga gcaggtgatg aagaccctga gcgaggtcta
      721 cccgcagaac acgccgaaga tcgccaccgg tttcagcgag gccgaattgc acgggcatac
      781 ccgcatcctc gcctttaccc ctatcaaggg cctgccttcg gtgacctggt acctggcgct
      841 gtccatcgac aaggacaagg cctacgccat gctcagcaag ttccgcgtct cggccatcgc
      901 cgccgcgctg atctccatcg tcgccatcct ggtcctgctc ggcctgctga tccgcctgct
      961 gatgcagccg ctgcacctga tgggccgtgc catgcaggac atcgcccagg gcgagggcga
     1021 cctgaccaag cgcctcgccg tgaccagccg cgatgagttc ggagtgctcg gcgatgcctt
     1081 caaccagttc gtcgagcgta tccatcgctc gatccgggaa gtccgcgaag gttcagatgc
     1141 catctatgcc ggtacccgtg aaattgcggc gggcaacacc gatctttcct cccgtactga
     1201 acagcaggca tccgcgctgg aagaaactgc cgccagcatg gagcagctca ccgcgacagt
     1261 gaagcaaaac gccgataacg cccgccaggc ctcgcaactg gcgcaaagtg cctccgacac
     1321 cgcccagcac ggcggcaaag tggtggatgg cgtagtgaaa acgatgcatg agatcgccga
     1381 tagttcgaag aaaattgccg acattatcag cgttatcgac ggtattgcct tccagactaa
     1441 tatcctcgcg ctgaatgccg cggttgaagc cgcgcgtgcg ggtgaacagg gccgtggttt
     1501 tgccgtggtg gcgggtgaag tgcgtaatct tgccagtcgc agcgcccagg cggcaaaaga
     1561 gatcaaagcc ctcattgaag actccgtctc acgcgttgat accggttcgg tgctggtcga
     1621 aagcgccggg gaaacaatga acaatatcgt caatgctgtc actcgcgtga ctgacattat
     1681 gggcgagatt gcatcggcat cggatgaaca gagccgtggc atcgatcaag tcgcattggc
     1741 ggtttcggaa atggatcgcg tcacgcaaca gaacgcatcg ctggtgcagg aatcagctgc
     1801 cgccgccgct gcgctggaag aacaggcgag tcgtttaacg caagcagttt ccgcgttccg
     1861 tctggcagcc agcccactca ccaataaacc gcaaacacca tcccgtcctg ccagtgagca
     1921 accaccggct cagccacgac tgcgaattgc tgaacaagat ccaaactggg aaacattttg
     1981 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     2041 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     2101 ggcctttctg cgtttata
//