LOCUS       BBa_K1992008            2610 bp    DNA     linear       28-Jun-2024
DEFINITION  Tar GFP tagged, native RBS expression system (promoter+RBS+coding+t
            erminator)
ACCESSION   BBa_K1992008
VERSION     BBa_K1992008.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     RBS             44..67
                     /label=Tar native RBS
     RBS             44..67
                     /label=BBa_K1992000
     misc_feature    74..76
                     /label=ATG
     CDS             74..1735
                     /label=Tar coding region
     misc_feature    1733..1735
                     /label=TGA
     CDS             74..1735
                     /label=BBa_K777000
     misc_feature    1736..1753
                     /label=BBa_J18921
     CDS             1754..2473
                     /label=GFP protein
     CDS             1754..2473
                     /label=BBa_E0040
     misc_feature    74..2473
                     /label=BBa_K1992003
     stem_loop       2493..2536
                     /label=stem_loop
     misc_feature    2482..2561
                     /label=BBa_B0010
     terminator      2482..2561
                     /label=BBa_B0010
     stem_loop       2577..2596
                     /label=T7 TE
     misc_feature    2603..2603
                     /label=stop
     polyA_site      2597..2610
                     /label=polya
     misc_feature    2570..2610
                     /label=BBa_B0012
     terminator      2570..2610
                     /label=BBa_B0012
     terminator      2482..2610
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaacttgt tttcaggaag
       61 gtgcctttac tagatgatta accgtatccg cgtagtcacg ctgttggtaa tggtgctggg
      121 ggtattcgca ctgttacagc ttatttccgg cagtctgttt ttttcttccc ttcaccatag
      181 ccagaagagc tttgtggttt ccaatcaatt acgggaacag cagggcgagc tgacgtcaac
      241 ctgggattta atgctgcaaa cgcgcattaa cctgagtcgt tcagcggtac ggatgatgat
      301 ggattcctcc aatcaacaaa gtaacgccaa agttgaattg ctcgatagcg ccaggaaaac
      361 attggcgcag gcagcgacgc attataaaaa attcaaaagc atggcaccgt tacctgaaat
      421 ggtcgctacc agtcgtaata ttgatgaaaa atataaaaac tattacacag cgttaactga
      481 actgattgat tacctagatt atggcaatac tggagcttat ttcgctcagc caacccaggg
      541 aatgcaaaat gcaatgggcg aagcgtttgc tcagtacgcc ctcagcagtg aaaaactgta
      601 tcgcgatatc gtcactgaca acgcagatga ttaccgattt gcccagtggc aactggcggt
      661 tatcgcgctg gtggtggtat tgattctgct ggtggcgtgg tacggcattc gccgtatgtt
      721 gcttactccg ctggcaaaaa ttattgctca cattcgcgaa atcgccggtg gtaacctggc
      781 gaataccctg accattgacg ggcgcagtga aatgggcgac ctggcgcaga gcgtttcaca
      841 tatgcaacgc tctttgactg acaccgtcac tcatgtccgc gaaggttcag atgccatcta
      901 tgccggtacc cgtgaaattg cggcgggcaa caccgatctt tcctcccgta ctgaacagca
      961 ggcatccgcg ctggaagaaa ctgccgccag catggagcag ctcaccgcga cagtgaagca
     1021 aaacgccgat aacgcccgcc aggcctcgca actggcgcaa agtgcctccg acaccgccca
     1081 gcacggcggc aaagtggtgg atggcgtagt gaaaacgatg catgagatcg ccgatagttc
     1141 gaagaaaatt gccgacatta tcagcgttat cgacggtatt gccttccaga ctaatatcct
     1201 cgcgctgaat gccgcggttg aagccgcgcg tgcgggtgaa cagggccgtg gttttgccgt
     1261 ggtggcgggt gaagtgcgta atcttgccag tcgcagcgcc caggcggcaa aagagatcaa
     1321 agccctcatt gaagactccg tctcacgcgt tgataccggt tcggtgctgg tcgaaagcgc
     1381 cggggaaaca atgaacaata tcgtcaatgc tgtcactcgc gtgactgaca ttatgggcga
     1441 gattgcatcg gcatcggatg aacagagccg tggcatcgat caagtcgcat tggcggtttc
     1501 ggaaatggat cgcgtcacgc aacagaacgc atcgctggtg caggaatcag ctgccgccgc
     1561 cgctgcgctg gaagaacagg cgagtcgttt aacgcaagca gtttccgcgt tccgtctggc
     1621 agccagccca ctcaccaata aaccgcaaac accatcccgt cctgccagtg agcaaccacc
     1681 ggctcagcca cgactgcgaa ttgctgaaca agatccaaac tgggaaacat tttgaggtag
     1741 cggcagcggt agcatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt
     1801 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
     1861 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
     1921 atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga
     1981 tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag
     2041 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg
     2101 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat
     2161 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa
     2221 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt
     2281 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc
     2341 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga
     2401 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact
     2461 atacaaataa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     2521 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     2581 caccttcggg tgggcctttc tgcgtttata
//