LOCUS       BBa_K1992009            2598 bp    DNA     linear       16-Jun-2024
DEFINITION  Tar GFP tagged  expression system (promoter+RBS+coding+terminator)
ACCESSION   BBa_K1992009
VERSION     BBa_K1992009.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    62..64
                     /label=ATG
     CDS             62..1723
                     /label=Tar coding region
     misc_feature    1721..1723
                     /label=TGA
     CDS             62..1723
                     /label=BBa_K777000
     misc_feature    1724..1741
                     /label=BBa_J18921
     CDS             1742..2461
                     /label=GFP protein
     CDS             1742..2461
                     /label=BBa_E0040
     misc_feature    62..2461
                     /label=BBa_K1992003
     stem_loop       2481..2524
                     /label=stem_loop
     misc_feature    2470..2549
                     /label=BBa_B0010
     terminator      2470..2549
                     /label=BBa_B0010
     stem_loop       2565..2584
                     /label=T7 TE
     misc_feature    2591..2591
                     /label=stop
     polyA_site      2585..2598
                     /label=polya
     misc_feature    2558..2598
                     /label=BBa_B0012
     terminator      2558..2598
                     /label=BBa_B0012
     terminator      2470..2598
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgattaac cgtatccgcg tagtcacgct gttggtaatg gtgctggggg tattcgcact
      121 gttacagctt atttccggca gtctgttttt ttcttccctt caccatagcc agaagagctt
      181 tgtggtttcc aatcaattac gggaacagca gggcgagctg acgtcaacct gggatttaat
      241 gctgcaaacg cgcattaacc tgagtcgttc agcggtacgg atgatgatgg attcctccaa
      301 tcaacaaagt aacgccaaag ttgaattgct cgatagcgcc aggaaaacat tggcgcaggc
      361 agcgacgcat tataaaaaat tcaaaagcat ggcaccgtta cctgaaatgg tcgctaccag
      421 tcgtaatatt gatgaaaaat ataaaaacta ttacacagcg ttaactgaac tgattgatta
      481 cctagattat ggcaatactg gagcttattt cgctcagcca acccagggaa tgcaaaatgc
      541 aatgggcgaa gcgtttgctc agtacgccct cagcagtgaa aaactgtatc gcgatatcgt
      601 cactgacaac gcagatgatt accgatttgc ccagtggcaa ctggcggtta tcgcgctggt
      661 ggtggtattg attctgctgg tggcgtggta cggcattcgc cgtatgttgc ttactccgct
      721 ggcaaaaatt attgctcaca ttcgcgaaat cgccggtggt aacctggcga ataccctgac
      781 cattgacggg cgcagtgaaa tgggcgacct ggcgcagagc gtttcacata tgcaacgctc
      841 tttgactgac accgtcactc atgtccgcga aggttcagat gccatctatg ccggtacccg
      901 tgaaattgcg gcgggcaaca ccgatctttc ctcccgtact gaacagcagg catccgcgct
      961 ggaagaaact gccgccagca tggagcagct caccgcgaca gtgaagcaaa acgccgataa
     1021 cgcccgccag gcctcgcaac tggcgcaaag tgcctccgac accgcccagc acggcggcaa
     1081 agtggtggat ggcgtagtga aaacgatgca tgagatcgcc gatagttcga agaaaattgc
     1141 cgacattatc agcgttatcg acggtattgc cttccagact aatatcctcg cgctgaatgc
     1201 cgcggttgaa gccgcgcgtg cgggtgaaca gggccgtggt tttgccgtgg tggcgggtga
     1261 agtgcgtaat cttgccagtc gcagcgccca ggcggcaaaa gagatcaaag ccctcattga
     1321 agactccgtc tcacgcgttg ataccggttc ggtgctggtc gaaagcgccg gggaaacaat
     1381 gaacaatatc gtcaatgctg tcactcgcgt gactgacatt atgggcgaga ttgcatcggc
     1441 atcggatgaa cagagccgtg gcatcgatca agtcgcattg gcggtttcgg aaatggatcg
     1501 cgtcacgcaa cagaacgcat cgctggtgca ggaatcagct gccgccgccg ctgcgctgga
     1561 agaacaggcg agtcgtttaa cgcaagcagt ttccgcgttc cgtctggcag ccagcccact
     1621 caccaataaa ccgcaaacac catcccgtcc tgccagtgag caaccaccgg ctcagccacg
     1681 actgcgaatt gctgaacaag atccaaactg ggaaacattt tgaggtagcg gcagcggtag
     1741 catgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
     1801 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
     1861 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
     1921 tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca
     1981 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
     2041 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt
     2101 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
     2161 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg
     2221 aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga
     2281 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
     2341 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct
     2401 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata
     2461 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     2521 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     2581 ggcctttctg cgtttata
//