LOCUS       BBa_K199201             1212 bp    DNA     linear       26-Nov-2024
DEFINITION  pLac-RBS-ATG-loxP-STOP-loxP-RFP
ACCESSION   BBa_K199201
VERSION     BBa_K199201.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     misc_feature    227..484
                     /label=BBa_K199199
     misc_feature    493..526
                     /label=BBa_J61046
     misc_feature    227..526
                     /label=BBa_S04537
     misc_feature    1..526
                     /label=BBa_K199200
     misc_feature    535..1212
                     /label=BBa_K199197
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga taacttcgta
      241 taatgtatgc tatacgaagt tataaggcct gattatgaag agcgtgtgac agagacacat
      301 agaatatctc acacgacatg gcagttaaat aataaaaaag ccggattaat aatctggctt
      361 tttattattc tctctctagt atataaacgc agaaaggccc acccgaaggt gagccagcgt
      421 gaactgccaa tctgggttaa gttatcttag gttttctgca ccgcgccttc tttaatcagg
      481 cctttactag agataacttc gtataatgta tgctatacga agttattact agaggcttcc
      541 tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac
      601 ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc
      661 gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat cctgtccccg
      721 cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc ggactacctg
      781 aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga agacggtggt
      841 gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta caaagttaaa
      901 ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac catgggttgg
      961 gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg
     1021 cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct
     1081 aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc
     1141 cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc
     1201 ggtgcttaat aa
//