LOCUS       BBa_K1992010            2870 bp    DNA     linear       22-Nov-2024
DEFINITION  PctA-Tar GFP tagged expression system (promoter+RBS+coding+terminat
            or)
ACCESSION   BBa_K1992010
VERSION     BBa_K1992010.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..1123
                     /label=PctA LBD + HAMP
     CDS             1124..1980
                     /label=Tar signaling domain
     misc_feature    160..160
                     /label=c
     misc_feature    463..463
                     /label=a
     misc_feature    466..466
                     /label=a
     misc_feature    1057..1057
                     /label=g
     CDS             62..1981
                     /label=BBa_K1992001
     misc_feature    1990..2007
                     /label=BBa_J18921
     CDS             2014..2733
                     /label=GFP protein
     CDS             2014..2733
                     /label=BBa_E0040
     stem_loop       2753..2796
                     /label=stem_loop
     misc_feature    2742..2821
                     /label=BBa_B0010
     terminator      2742..2821
                     /label=BBa_B0010
     stem_loop       2837..2856
                     /label=T7 TE
     misc_feature    2863..2863
                     /label=stop
     polyA_site      2857..2870
                     /label=polya
     misc_feature    2830..2870
                     /label=BBa_B0012
     terminator      2830..2870
                     /label=BBa_B0012
     terminator      2742..2870
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgatcaaa agtctgaagt tcagccacaa gatcctcctc gcggcctccc tcgtggtgtt
      121 cgccgctttc gcactgttca ccctctacaa cgattacctc cagcgcaacg cgatccgcga
      181 ggacctcgaa agctacctgc gggaaatggg tgacgtgact tccagcaaca ttcagaactg
      241 gctcggcggc cgtctgctgc tggtcgagca gaccgcccag accctggccc gcgaccacag
      301 cccggaaacc gtcagcgccc tgctcgaaca gccggccctg acctcgacct tcagcttcac
      361 ctacctcggc cagcaggacg gcgtcttcac catgcgtccg gacagcccga tgccggccgg
      421 ctacgatccg cgcagccggc cctggtacaa ggacgccgtg gcagcaggcg gcctgaccct
      481 gaccgaaccc tacgtcgacg ccgccaccca ggaattgatc atcaccgcgg cgacaccggt
      541 gaaggccgct ggcaacaccc tcggcgtggt aggcggcgac ctcagcctga agaccctggt
      601 gcagatcatc aattcgctgg acttcagcgg catgggctac gccttcctgg tcagcggcga
      661 cggcaagatc ctggtgcacc cggacaaaga gcaggtgatg aagaccctga gcgaggtcta
      721 cccgcagaac acgccgaaga tcgccaccgg tttcagcgag gccgaattgc acgggcatac
      781 ccgcatcctc gcctttaccc ctatcaaggg cctgccttcg gtgacctggt acctggcgct
      841 gtccatcgac aaggacaagg cctacgccat gctcagcaag ttccgcgtct cggccatcgc
      901 cgccgcgctg atctccatcg tcgccatcct ggtcctgctc ggcctgctga tccgcctgct
      961 gatgcagccg ctgcacctga tgggccgtgc catgcaggac atcgcccagg gcgagggcga
     1021 cctgaccaag cgcctcgccg tgaccagccg cgatgagttc ggagtgctcg gcgatgcctt
     1081 caaccagttc gtcgagcgta tccatcgctc gatccgggaa gtccgcgaag gttcagatgc
     1141 catctatgcc ggtacccgtg aaattgcggc gggcaacacc gatctttcct cccgtactga
     1201 acagcaggca tccgcgctgg aagaaactgc cgccagcatg gagcagctca ccgcgacagt
     1261 gaagcaaaac gccgataacg cccgccaggc ctcgcaactg gcgcaaagtg cctccgacac
     1321 cgcccagcac ggcggcaaag tggtggatgg cgtagtgaaa acgatgcatg agatcgccga
     1381 tagttcgaag aaaattgccg acattatcag cgttatcgac ggtattgcct tccagactaa
     1441 tatcctcgcg ctgaatgccg cggttgaagc cgcgcgtgcg ggtgaacagg gccgtggttt
     1501 tgccgtggtg gcgggtgaag tgcgtaatct tgccagtcgc agcgcccagg cggcaaaaga
     1561 gatcaaagcc ctcattgaag actccgtctc acgcgttgat accggttcgg tgctggtcga
     1621 aagcgccggg gaaacaatga acaatatcgt caatgctgtc actcgcgtga ctgacattat
     1681 gggcgagatt gcatcggcat cggatgaaca gagccgtggc atcgatcaag tcgcattggc
     1741 ggtttcggaa atggatcgcg tcacgcaaca gaacgcatcg ctggtgcagg aatcagctgc
     1801 cgccgccgct gcgctggaag aacaggcgag tcgtttaacg caagcagttt ccgcgttccg
     1861 tctggcagcc agcccactca ccaataaacc gcaaacacca tcccgtcctg ccagtgagca
     1921 accaccggct cagccacgac tgcgaattgc tgaacaagat ccaaactggg aaacattttg
     1981 atactagagg gtagcggcag cggtagctac tagatgcgta aaggagaaga acttttcact
     2041 ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc
     2101 agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact
     2161 actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa
     2221 tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc
     2281 gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt
     2341 gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat
     2401 tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat
     2461 gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac
     2521 aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc
     2581 gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa
     2641 gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt
     2701 acacatggca tggatgaact atacaaataa taatactaga gccaggcatc aaataaaacg
     2761 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     2821 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//