BBa_K1992010
type: ComponentDefinition
persistent identity: BBa K1992010
display id: BBa_K1992010
version: 1
was generated by: iGEM to SBOL conversion
description: PctA-Tar GFP tagged expression system (promoter+RBS+coding+terminator)
created: 2016-10-13T11:00:00Z
modified: 2016-10-18T03:40:03Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: Introduction A GFP gene was fused to the PctA chimera. The plasmid is comprised of a promoter, a strong RBS, the chimera, a GFP gene and a terminator. In addition, a proper linker ought to be introduced in order to ensure the right correct fold of the protein. Usage and Biology This device was used as a proof of concept in order to verify the migration of the new chemoreceptor to the poles of the bacterial membrane. Design Considerations A flexible linker was introduced to fuse the GFP gene to the chimera. The linker is essential for the protein to fold in the right manner and for the correct tertiary structure to be obtained. For methods and results please go to PctA-Tar (K1992001)
discontinued: false
dominant: false
experience: experience/Works
group u list: _2459_
m user id: 30463
owner id: 30463
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Shilo Ohayon
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_J23100
component: BBa_K1992001
component: BBa_B0034
component: BBa_E0040
component: BBa_J18921
component: BBa_B0015
sequence annotation: BBa_J23100
sequence annotation: BBa_B0034
sequence annotation: BBa_K1992001
sequence annotation: BBa_J18921
sequence annotation: BBa_E0040
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1992010_sequence/1

BBa_J23100
type: Component
persistent identity: component2501975
display id: component2501975
version: 1
top level: BBa_K1992010
definition: BBa_J23100
access: public

BBa_K1992001
type: Component
persistent identity: component2501979
display id: component2501979
version: 1
top level: BBa_K1992010
definition: BBa_K1992001
access: public

BBa_B0034
type: Component
persistent identity: component2501977
display id: component2501977
version: 1
top level: BBa_K1992010
definition: BBa_B0034
access: public

BBa_E0040
type: Component
persistent identity: component2501982
display id: component2501982
version: 1
top level: BBa_K1992010
definition: GFP
access: public

BBa_J18921
type: Component
persistent identity: component2501980
display id: component2501980
version: 1
top level: BBa_K1992010
definition: 3xGS
access: public

BBa_B0015
type: Component
persistent identity: component2501989
display id: component2501989
version: 1
top level: BBa_K1992010
definition: BBa_B0015
access: public

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2501975
display id: annotation2501975
version: 1
top level: BBa_K1992010
location: https://synbiohub.org/public/igem/BBa_K1992010/annotation2501975/range2501975/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1992010/annotation2501975/range2501975/1
type: Range
persistent identity: range2501975
display id: range2501975
version: 1
top level: BBa_K1992010
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2501977
display id: annotation2501977
version: 1
top level: BBa_K1992010
location: https://synbiohub.org/public/igem/BBa_K1992010/annotation2501977/range2501977/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1992010/annotation2501977/range2501977/1
type: Range
persistent identity: range2501977
display id: range2501977
version: 1
top level: BBa_K1992010
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_K1992001
type: SequenceAnnotation
persistent identity: annotation2501979
display id: annotation2501979
version: 1
top level: BBa_K1992010
location: https://synbiohub.org/public/igem/BBa_K1992010/annotation2501979/range2501979/1
component: BBa_K1992001

https://synbiohub.org/public/igem/BBa_K1992010/annotation2501979/range2501979/1
type: Range
persistent identity: range2501979
display id: range2501979
version: 1
top level: BBa_K1992010
direction: direction/reverse
start: 62
end: 1981
orientation: inline

BBa_J18921
type: SequenceAnnotation
persistent identity: annotation2501980
display id: annotation2501980
version: 1
top level: BBa_K1992010
location: https://synbiohub.org/public/igem/BBa_K1992010/annotation2501980/range2501980/1
component: BBa_J18921

https://synbiohub.org/public/igem/BBa_K1992010/annotation2501980/range2501980/1
type: Range
persistent identity: range2501980
display id: range2501980
version: 1
top level: BBa_K1992010
direction: direction/reverse
start: 1990
end: 2007
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2501982
display id: annotation2501982
version: 1
top level: BBa_K1992010
location: https://synbiohub.org/public/igem/BBa_K1992010/annotation2501982/range2501982/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K1992010/annotation2501982/range2501982/1
type: Range
persistent identity: range2501982
display id: range2501982
version: 1
top level: BBa_K1992010
direction: direction/reverse
start: 2014
end: 2733
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2501989
display id: annotation2501989
version: 1
top level: BBa_K1992010
location: https://synbiohub.org/public/igem/BBa_K1992010/annotation2501989/range2501989/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1992010/annotation2501989/range2501989/1
type: Range
persistent identity: range2501989
display id: range2501989
version: 1
top level: BBa_K1992010
direction: direction/reverse
start: 2742
end: 2870
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

3xGS
type: ComponentDefinition
persistent identity: BBa J18921
display id: BBa_J18921
version: 1
was generated by: iGEM to SBOL conversion
description: 6aa [GS]x linker
created: 2010-01-26T12:00:00Z
modified: 2015-08-31T04:08:36Z
mutable provenance: gene synthesis
owned by: james
owned by: myers
mutable description: Highly flexible 6 amino acid linker. Translates to gsgsgs. Codon-optimize for E. coli, yeast, mammalian.
discontinued: false
dominant: false
experience: experience/None
group u list: _165_
m user id: 0
owner id: 2175
owning group id: 165
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: * gene synthesis * codon optimized for E. coli
star: false
creator: Raik Gruenberg
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J18921_sequence/1

BBa_K1992001
type: ComponentDefinition
persistent identity: BBa K1992001
display id: BBa_K1992001
version: 1
was generated by: iGEM to SBOL conversion
description: PctA-Tar hybrid receptor
created: 2016-10-13T11:00:00Z
modified: 2016-10-18T03:07:56Z
mutable provenance: The sequence for the desired segment of PctA was taken from the Pseudomonas genome database http://www.pseudomonas.com/feature/show?id=111456. Tar???s cytoplasmic region, was obtained from the iGEM parts catalog
owned by: james
owned by: myers
mutable description: PctA-Tar Introduction PctA is a chemoreceptor found in the bacterium Pseudomonas Aeruginosa which mediates chemotaxis toward amino acids and away from organic compounds. It is sensitive to all amino acids accept for Aspartate (1). A PctA-Tar chimera was formed by the replacement of the Tar LBD with the one of PctA. A procedure which previously was conducted in the literature was recovered (1), in order to proof the concept of the S. Tar platform. Usage and biology Integration of the new chemoreceptor with the chemotaxis system of E. coli is enabled thank our chimera design. Hence, a chemotactic attractant response to new amino acids and a repellent response to organic compounds, which weren't detected by E. coli before, are now mediated. Design considerations When designing a gBlock for the PctA-Tar Chimera an incompatible part is obtained, this according to the IDT gBlock sequence online analyzer (2). As a result, a split part has to be designed. Therefore it is recommended to use the part itself, since its synthesis is problematic. Methods The sequence for the desired segment of PctA was taken from the Pseudomonas genome database (3). The second part of the chimera, Tar???s cytoplasmatic region, was obtained from the iGEM parts catalog. Amino acids sequence of the LBD and HAMP of PctA was determined to be from 1 to 134 and this of the signaling region of Tar was determined to be 296-553 (Figure 1a). Figure 1: (a) Amino acids sequence of PctA-Tar chimera (1) (b) PctA-Tar chimera crystalogaphy The plasmid carrying the chimera was transformed into receptorless bacteria- UU1250 (Parkinson J S, University of Utah) and the presents of the DNA sequence was verified by a colony PCR and sequencing. In order to test the motility of our strain, chemotaxis assays were performed. First, swarming assay was conducted using a poor BA medium. Note that with a TB medium the strain failed to show decisive results, provably due to high concentration of amino acid which resulted in no…
discontinued: false
dominant: false
experience: experience/Works
group u list: _2459_
m user id: 30463
owner id: 30463
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: gBlocks were not compatible so we had to split them into two fragments
star: false
creator: Shilo Ohayon,Asif Gil
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: PctA LBD + HAMP
sequence annotation: c
sequence annotation: a
sequence annotation: g
sequence annotation: Tar signaling domain
sequence: https://synbiohub.org/public/igem/BBa_K1992001_sequence/1

PctA LBD + HAMP
type: SequenceAnnotation
persistent identity: annotation2501346
display id: annotation2501346
version: 1
top level: BBa_K1992001
location: https://synbiohub.org/public/igem/BBa_K1992001/annotation2501346/range2501346/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1992001/annotation2501346/range2501346/1
type: Range
persistent identity: range2501346
display id: range2501346
version: 1
top level: BBa_K1992001
direction: direction/forward
start: 1
end: 1062
orientation: inline

c
type: SequenceAnnotation
persistent identity: annotation2521691
display id: annotation2521691
version: 1
top level: BBa_K1992001
location: https://synbiohub.org/public/igem/BBa_K1992001/annotation2521691/range2521691/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_K1992001/annotation2521691/range2521691/1
type: Range
persistent identity: range2521691
display id: range2521691
version: 1
top level: BBa_K1992001
direction: direction/forward
start: 99
end: 99
orientation: inline

a
type: SequenceAnnotation
persistent identity: annotation2521700
display id: annotation2521700
version: 1
top level: BBa_K1992001
location: https://synbiohub.org/public/igem/BBa_K1992001/annotation2521700/range2521700/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_K1992001/annotation2521700/range2521700/1
type: Range
persistent identity: range2521700
display id: range2521700
version: 1
top level: BBa_K1992001
direction: direction/forward
start: 402
end: 402
orientation: inline

a
type: SequenceAnnotation
persistent identity: annotation2521701
display id: annotation2521701
version: 1
top level: BBa_K1992001
location: https://synbiohub.org/public/igem/BBa_K1992001/annotation2521701/range2521701/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_K1992001/annotation2521701/range2521701/1
type: Range
persistent identity: range2521701
display id: range2521701
version: 1
top level: BBa_K1992001
direction: direction/forward
start: 405
end: 405
orientation: inline

g
type: SequenceAnnotation
persistent identity: annotation2522553
display id: annotation2522553
version: 1
top level: BBa_K1992001
location: https://synbiohub.org/public/igem/BBa_K1992001/annotation2522553/range2522553/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K1992001/annotation2522553/range2522553/1
type: Range
persistent identity: range2522553
display id: range2522553
version: 1
top level: BBa_K1992001
direction: direction/forward
start: 996
end: 996
orientation: inline

Tar signaling domain
type: SequenceAnnotation
persistent identity: annotation2521679
display id: annotation2521679
version: 1
top level: BBa_K1992001
location: https://synbiohub.org/public/igem/BBa_K1992001/annotation2521679/range2521679/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1992001/annotation2521679/range2521679/1
type: Range
persistent identity: range2521679
display id: range2521679
version: 1
top level: BBa_K1992001
direction: direction/forward
start: 1063
end: 1919
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1992010_sequence/1
type: Sequence
persistent identity: BBa K1992010 sequence
display id: BBa_K1992010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgatcaaaagtctgaagttcagccacaagatcctcctcgcggcctccctcgtggtgttcgccgctttcgcactgttcaccctctacaacgattacctccagcgcaacgcgatccgcgaggacctcgaaagctacctgcgggaaatgggtgacgtgacttccagcaacattcagaactggctcggcggccgtctgctgctggtcgagcagaccgcccagaccctggcccgcgaccacagcccggaaaccgtcagcgccctgctcgaacagccggccctgacctcgaccttcagcttcacctacctcggccagcaggacggcgtcttcaccatgcgtccggacagcccgatgccggccggctacgatccgcgcagccggccctggtacaaggacgccgtggcagcaggcggcctgaccctgaccgaaccctacgtcgacgccgccacccaggaattgatcatcaccgcggcgacaccggtgaaggccgctggcaacaccctcggcgtggtaggcggcgacctcagcctgaagaccctggtgcagatcatcaattcgctggacttcagcggcatgggctacgccttcctggtcagcggcgacggcaagatcctggtgcacccggacaaagagcaggtgatgaagaccctgagcgaggtctacccgcagaacacgccgaagatcgccaccggtttcagcgaggccgaattgcacgggcatacccgcatcctcgcctttacccctatcaagggcctgccttcggtgacctggtacctggcgctgtccatcgacaaggacaaggcctacgccatgctcagcaagttccgcgtctcggccatcgccgccgcgctgatctccatcgtcgccatcctggtcctgctcggcctgctgatccgcctgctgatgcagccgctgcacctgatgggccgtgccatgcaggacatcgcccagggcgagggcgacctgaccaagcgcctcgccgtgaccagccgcgatgagttcggagtgctcggcgatgccttcaaccagttcgtcgagcgtatccatcgctcgatccgggaagtccgcgaaggttcagatgccatctatgccggtacccgtgaaattgcggcgggcaacaccgatctttcctcccgtactgaacagcaggcatccgcgctggaagaaactgccgccagcatggagcagctcaccgcgacagtgaagcaaaacgccgataacgcccgccaggcctcgcaactggcgcaaagtgcctccgacaccgcccagcacggcggcaaagtggtggatggcgtagtgaaaacgatgcatgagatcgccgatagttcgaagaaaattgccgacattatcagcgttatcgacggtattgccttccagactaatatcctcgcgctgaatgccgcggttgaagccgcgcgtgcgggtgaacagggccgtggttttgccgtggtggcgggtgaagtgcgtaatcttgccagtcgcagcgcccaggcggcaaaagagatcaaagccctcattgaagactccgtctcacgcgttgataccggttcggtgctggtcgaaagcgccggggaaacaatgaacaatatcgtcaatgctgtcactcgcgtgactgacattatgggcgagattgcatcggcatcggatgaacagagccgtggcatcgatcaagtcgcattggcggtttcggaaatggatcgcgtcacgcaacagaacgcatcgctggtgcaggaatcagctgccgccgccgctgcgctggaagaacaggcgagtcgtttaacgcaagcagtttccgcgttccgtctggcagccagcccactcaccaataaaccgcaaacaccatcccgtcctgccagtgagcaaccaccggctcagccacgactgcgaattgctgaacaagatccaaactgggaaacattttgatactagagggtagcggcag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J18921_sequence/1
type: Sequence
persistent identity: BBa J18921 sequence
display id: BBa_J18921_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtagcggcagcggtagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1992001_sequence/1
type: Sequence
persistent identity: BBa K1992001 sequence
display id: BBa_K1992001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatcaaaagtctgaagttcagccacaagatcctcctcgcggcctccctcgtggtgttcgccgctttcgcactgttcaccctctacaacgattacctccagcgcaacgcgatccgcgaggacctcgaaagctacctgcgggaaatgggtgacgtgacttccagcaacattcagaactggctcggcggccgtctgctgctggtcgagcagaccgcccagaccctggcccgcgaccacagcccggaaaccgtcagcgccctgctcgaacagccggccctgacctcgaccttcagcttcacctacctcggccagcaggacggcgtcttcaccatgcgtccggacagcccgatgccggccggctacgatccgcgcagccggccctggtacaaggacgccgtggcagcaggcggcctgaccctgaccgaaccctacgtcgacgccgccacccaggaattgatcatcaccgcggcgacaccggtgaaggccgctggcaacaccctcggcgtggtaggcggcgacctcagcctgaagaccctggtgcagatcatcaattcgctggacttcagcggcatgggctacgccttcctggtcagcggcgacggcaagatcctggtgcacccggacaaagagcaggtgatgaagaccctgagcgaggtctacccgcagaacacgccgaagatcgccaccggtttcagcgaggccgaattgcacgggcatacccgcatcctcgcctttacccctatcaagggcctgccttcggtgacctggtacctggcgctgtccatcgacaaggacaaggcctacgccatgctcagcaagttccgcgtctcggccatcgccgccgcgctgatctccatcgtcgccatcctggtcctgctcggcctgctgatccgcctgctgatgcagccgctgcacctgatgggccgtgccatgcaggacatcgcccagggcgagggcgacctgaccaagcgcctcgccgtgaccagccgcgatgagttcggagtgctcggcgatgccttcaaccagttcgtcgagcgtatccatcgctcgatccgggaagtccgcgaaggttcagatgccatctatgccggtacccgtgaaattgcggcgggcaacaccgatctttcctcccgtactgaacagcaggcatccgcgctggaagaaactgccgccagcatggagcagctcaccgcgacagtgaagcaaaacgccgataacgcccgccaggcctcgcaactggcgcaaagtgcctccgacaccgcccagcacggcggcaaagtggtggatggcgtagtgaaaacgatgcatgagatcgccgatagttcgaagaaaattgccgacattatcagcgttatcgacggtattgccttccagactaatatcctcgcgctgaatgccgcggttgaagccgcgcgtgcgggtgaacagggccgtggttttgccgtggtggcgggtgaagtgcgtaatcttgccagtcgcagcgcccaggcggcaaaagagatcaaagccctcattgaagactccgtctcacgcgttgataccggttcggtgctggtcgaaagcgccggggaaacaatgaacaatatcgtcaatgctgtcactcgcgtgactgacattatgggcgagattgcatcggcatcggatgaacagagccgtggcatcgatcaagtcgcattggcggtttcggaaatggatcgcgtcacgcaacagaacgcatcgctggtgcaggaatcagctgccgccgccgctgcgctggaagaacaggcgagtcgtttaacgcaagcagtttccgcgttccgtctggcagccagcccactcaccaataaaccgcaaacaccatcccgtcctgccagtgagcaaccaccggctcagccacgactgcgaattgctgaacaagatccaaactgggaaacattttga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z