LOCUS       BBa_K1992011            1872 bp    DNA     linear       22-Nov-2024
DEFINITION  Histamin-Tar expression system(promoter+RBS+coding+terminator)
ACCESSION   BBa_K1992011
VERSION     BBa_K1992011.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     RBS             44..67
                     /label=Tar native RBS
     RBS             44..67
                     /label=BBa_K1992000
     misc_feature    323..338
                     /label=Region I mutations
     misc_feature    578..608
                     /label=Region II mutations
     CDS             108..670
                     /label=Ligand-Binding domain
     CDS             74..1735
                     /label=BBa_K1992002
     stem_loop       1755..1798
                     /label=stem_loop
     misc_feature    1744..1823
                     /label=BBa_B0010
     terminator      1744..1823
                     /label=BBa_B0010
     stem_loop       1839..1858
                     /label=T7 TE
     misc_feature    1865..1865
                     /label=stop
     polyA_site      1859..1872
                     /label=polya
     misc_feature    1832..1872
                     /label=BBa_B0012
     terminator      1832..1872
                     /label=BBa_B0012
     terminator      1744..1872
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaacttgt tttcaggaag
       61 gtgcctttac tagatgatta accgtatccg cgtagtcacg ctgttggtaa tggtgctggg
      121 ggtattcgca ctgttacagc ttatttccgg cagtctgttt ttttcttccc ttcaccatag
      181 ccagaagagc tttgtggttt ccaatcaatt acgggaacag cagggcgagc tgacgtcaac
      241 ctgggattta atgctgcaaa cggaaattaa cctgtggcgt tcagcggtac ggatgatgat
      301 ggattcctcc aatcaacaaa gtaacgccaa agttgaattg ctcgatagcg ccaggaaaac
      361 attggcgcag gcagcgacgc attataaaaa attcaaaagc atggcaccgt tacctgaaat
      421 ggtcgctacc agtcgtaata ttgatgaaaa atataaaaac tattacacag cgttaactga
      481 actgattgat tacctagatt atggcaatac tggagctgaa gtggctcatc caacctgggg
      541 aatgatgaat gcaatgggcg aagcgtttgc tcagtacgcc ctcagcagtg aaaaactgta
      601 tcgcgatatc gtcactgaca acgcagatga ttaccgattt gcccagtggc aactggcggt
      661 tatcgcgctg gtggtggtat tgattctgct ggtggcgtgg tacggcattc gccgtatgtt
      721 gcttactccg ctggcaaaaa ttattgctca cattcgcgaa atcgccggtg gtaacctggc
      781 gaataccctg accattgacg ggcgcagtga aatgggcgac ctggcgcaga gcgtttcaca
      841 tatgcaacgc tctttgactg acaccgtcac tcatgtccgc gaaggttcag atgccatcta
      901 tgccggtacc cgtgaaattg cggcgggcaa caccgatctt tcctcccgta ctgaacagca
      961 ggcatccgcg ctggaagaaa ctgccgccag catggagcag ctcaccgcga cagtgaagca
     1021 aaacgccgat aacgcccgcc aggcctcgca actggcgcaa agtgcctccg acaccgccca
     1081 gcacggcggc aaagtggtgg atggcgtagt gaaaacgatg catgagatcg ccgatagttc
     1141 gaagaaaatt gccgacatta tcagcgttat cgacggtatt gccttccaga ctaatatcct
     1201 cgcgctgaat gccgcggttg aagccgcgcg tgcgggtgaa cagggccgtg gttttgccgt
     1261 ggtggcgggt gaagtgcgta atcttgccag tcgcagcgcc caggcggcaa aagagatcaa
     1321 agccctcatt gaagactccg tctcacgcgt tgataccggt tcggtgctgg tcgaaagcgc
     1381 cggggaaaca atgaacaata tcgtcaatgc tgtcactcgc gtgactgaca ttatgggcga
     1441 gattgcatcg gcatcggatg aacagagccg tggcatcgat caagtcgcat tggcggtttc
     1501 ggaaatggat cgcgtcacgc aacagaacgc atcgctggtg caggaatcag ctgccgccgc
     1561 cgctgcgctg gaagaacagg cgagtcgttt aacgcaagca gtttccgcgt tccgtctggc
     1621 agccagccca ctcaccaata aaccgcaaac accatcccgt cctgccagtg agcaaccacc
     1681 ggctcagcca cgactgcgaa ttgctgaaca agatccaaac tgggaaacat tttgatacta
     1741 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     1801 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     1861 tctgcgttta ta
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