##gff-version 3
##sequence-region BBa_K1992011 1 1872
BBa_K1992011	.	CDS	74	1735	.	+	0	ID=BBa_K1992002;Name=BBa_K1992002
BBa_K1992011	.	CDS	108	670	.	+	0	ID=annotation2512517;Name=Ligand-Binding domain;Parent=BBa_K1992002
BBa_K1992011	.	sequence_alteration	578	608	.	+	0	ID=annotation2512515;Name=Region II mutations;Parent=BBa_K1992002
BBa_K1992011	.	sequence_alteration	323	338	.	+	0	ID=annotation2512509;Name=Region I mutations;Parent=BBa_K1992002
BBa_K1992011	.	ribosome_entry_site	44	67	.	+	0	ID=BBa_K1992000;Name=BBa_K1992000
BBa_K1992011	.	ribosome_entry_site	44	67	.	+	0	ID=annotation2501442;Name=Tar native RBS;Parent=BBa_K1992000
BBa_K1992011	.	terminator	1744	1872	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1992011	.	terminator	1832	1872	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1992011	.	stem_loop	1839	1858	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1992011	.	polyA_site	1859	1872	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1992011	.	stop_codon	1865	1865	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1992011	.	engineered_region	1832	1872	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1992011	.	terminator	1744	1823	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1992011	.	stem_loop	1755	1798	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1992011	.	engineered_region	1744	1823	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1992011	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100
>BBa_K1992011
ttgacggctagctcagtcctaggtacagtgctagctactagagaacttgttttcaggaaggtgcctttactagatgatt
aaccgtatccgcgtagtcacgctgttggtaatggtgctgggggtattcgcactgttacagcttatttccggcagtctgt
ttttttcttcccttcaccatagccagaagagctttgtggtttccaatcaattacgggaacagcagggcgagctgacgtc
aacctgggatttaatgctgcaaacggaaattaacctgtggcgttcagcggtacggatgatgatggattcctccaatcaa
caaagtaacgccaaagttgaattgctcgatagcgccaggaaaacattggcgcaggcagcgacgcattataaaaaattca
aaagcatggcaccgttacctgaaatggtcgctaccagtcgtaatattgatgaaaaatataaaaactattacacagcgtt
aactgaactgattgattacctagattatggcaatactggagctgaagtggctcatccaacctggggaatgatgaatgca
atgggcgaagcgtttgctcagtacgccctcagcagtgaaaaactgtatcgcgatatcgtcactgacaacgcagatgatt
accgatttgcccagtggcaactggcggttatcgcgctggtggtggtattgattctgctggtggcgtggtacggcattcg
ccgtatgttgcttactccgctggcaaaaattattgctcacattcgcgaaatcgccggtggtaacctggcgaataccctg
accattgacgggcgcagtgaaatgggcgacctggcgcagagcgtttcacatatgcaacgctctttgactgacaccgtca
ctcatgtccgcgaaggttcagatgccatctatgccggtacccgtgaaattgcggcgggcaacaccgatctttcctcccg
tactgaacagcaggcatccgcgctggaagaaactgccgccagcatggagcagctcaccgcgacagtgaagcaaaacgcc
gataacgcccgccaggcctcgcaactggcgcaaagtgcctccgacaccgcccagcacggcggcaaagtggtggatggcg
tagtgaaaacgatgcatgagatcgccgatagttcgaagaaaattgccgacattatcagcgttatcgacggtattgcctt
ccagactaatatcctcgcgctgaatgccgcggttgaagccgcgcgtgcgggtgaacagggccgtggttttgccgtggtg
gcgggtgaagtgcgtaatcttgccagtcgcagcgcccaggcggcaaaagagatcaaagccctcattgaagactccgtct
cacgcgttgataccggttcggtgctggtcgaaagcgccggggaaacaatgaacaatatcgtcaatgctgtcactcgcgt
gactgacattatgggcgagattgcatcggcatcggatgaacagagccgtggcatcgatcaagtcgcattggcggtttcg
gaaatggatcgcgtcacgcaacagaacgcatcgctggtgcaggaatcagctgccgccgccgctgcgctggaagaacagg
cgagtcgtttaacgcaagcagtttccgcgttccgtctggcagccagcccactcaccaataaaccgcaaacaccatcccg
tcctgccagtgagcaaccaccggctcagccacgactgcgaattgctgaacaagatccaaactgggaaacattttgatac
tagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaac
gctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata