LOCUS       BBa_K199202             1722 bp    DNA     linear       07-Dec-2025
DEFINITION  pLac-RBS-ATG-loxP-STOP-loxP-TetA
ACCESSION   BBa_K199202
VERSION     BBa_K199202.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     misc_feature    227..484
                     /label=BBa_K199199
     misc_feature    493..526
                     /label=BBa_J61046
     misc_feature    227..526
                     /label=BBa_S04537
     misc_feature    1..526
                     /label=BBa_K199200
     CDS             535..1722
                     /label=BBa_K199198
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga taacttcgta
      241 taatgtatgc tatacgaagt tataaggcct gattatgaag agcgtgtgac agagacacat
      301 agaatatctc acacgacatg gcagttaaat aataaaaaag ccggattaat aatctggctt
      361 tttattattc tctctctagt atataaacgc agaaaggccc acccgaaggt gagccagcgt
      421 gaactgccaa tctgggttaa gttatcttag gttttctgca ccgcgccttc tttaatcagg
      481 cctttactag agataacttc gtataatgta tgctatacga agttattact agagaaatct
      541 aacaatgcgc tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg cataggcttg
      601 gttatgccgg tactgccggg cctcttgcgg gatatcgtcc attccgacag catcgccagt
      661 cactatggcg tgctgctagc gctatatgcg ttgatgcaat ttctatgcgc acccgttctc
      721 ggagcactgt ccgaccgctt tggccgccgc ccagtcctgc tcgcttcgct acttggagcc
      781 actatcgact acgcgatcat ggcgaccaca cccgtcctgt ggatcctcta cgccggacgc
      841 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc
      901 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
      961 atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc
     1021 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag
     1081 tcgcataagg gagagcgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc
     1141 cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa
     1201 ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg
     1261 agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa
     1321 gccttcgtca ctggccccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc
     1381 atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc
     1441 ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg
     1501 ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct cgcggctctt
     1561 accagcctaa cttcgatcat tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg
     1621 agcacatgga acgggttggc atggattgta ggcgccgccc tataccttgt ctgcctcccc
     1681 gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct aa
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