LOCUS       BBa_K1993008            2273 bp    DNA     linear       22-Dec-2024
DEFINITION  Luciferase-IRES-eGFP
ACCESSION   BBa_K1993008
VERSION     BBa_K1993008.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=start codon
     CDS             4..933
                     /label=Luciferase (Renilla reniformis)
     misc_feature    934..936
                     /label=stop codon
     misc_feature    1..936
                     /label=BBa_K1993015
     misc_feature    937..965
                     /label=attB2
     RBS             966..1553
                     /label=IRES
     CDS             937..1553
                     /label=BBa_K1993023
     misc_feature    1554..1556
                     /label=start codon
     CDS             1557..2270
                     /label=eGFP
     misc_feature    2271..2273
                     /label=stop codon
     misc_feature    1554..2273
                     /label=BBa_K1993017
ORIGIN
        1 atgacttcga aagtttatga tccagaacaa aggaaacgga tgataactgg tccgcagtgg
       61 tgggccagat gtaaacaaat gaatgttctt gattcattta ttaattatta tgattcagaa
      121 aaacatgcag aaaatgctgt tattttttta catggtaacg cggcctcttc ttatttatgg
      181 cgacatgttg tgccacatat tgagccagta gcgcggtgta ttataccaga tcttattggt
      241 atgggcaaat caggcaaatc tggtaatggt tcttataggt tacttgatca ttacaaatat
      301 cttactgcat ggtttgaact tcttaattta ccaaagaaga tcatttttgt cggccatgat
      361 tggggtgctt gtttggcatt tcattatagc tatgagcatc aagataagat caaagcaata
      421 gttcacgctg aaagtgtagt agatgtgatt gaatcatggg atgaatggcc tgatattgaa
      481 gaagatattg cgttgatcaa atctgaagaa ggagaaaaaa tggttttgga gaataacttc
      541 ttcgtggaaa ccatgttgcc atcaaaaatc atgagaaagt tagaaccaga agaatttgca
      601 gcatatcttg aaccattcaa agagaaaggt gaagttcgtc gtccaacatt atcatggcct
      661 cgtgaaatcc cgttagtaaa aggtggtaaa cctgacgttg tacaaattgt taggaattat
      721 aatgcttatc tacgtgcaag tgatgattta ccaaaaatgt ttattgaatc ggatccagga
      781 ttcttttcca atgctattgt tgaaggcgcc aagaagtttc ctaatactga atttgtcaaa
      841 gtaaaaggtc ttcatttttc gcaagaagat gcacctgatg aaatgggaaa atatatcaaa
      901 tcgttcgttg agcgagttct caaaaatgaa caataagggg accactttgt acaagaaagc
      961 tgggtgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg
     1021 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     1081 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     1141 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     1201 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     1261 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     1321 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     1381 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     1441 tcggtacaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
     1501 ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca accatggtga
     1561 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     1621 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     1681 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     1741 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     1801 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     1861 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     1921 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     1981 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     2041 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     2101 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     2161 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     2221 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taa
//