LOCUS BBa_K1993010 3443 bp DNA linear 23-Dec-2024 DEFINITION CXCR5-T2A-Luciferase-IRES-eGFP ACCESSION BBa_K1993010 VERSION BBa_K1993010.1 FEATURES Location/Qualifiers misc_feature 1..3 /label=start codon for CXCR5 CDS 4..1116 /label=CXCR5 misc_feature 1117..1170 /label=T2A misc_feature 1171..1173 /label=start codon for Luciferase CDS 1174..2103 /label=Luciferase misc_feature 2104..2106 /label=stop codon for Luciferase CDS 1..2106 /label=BBa_K1993033 misc_feature 2107..2135 /label=attB2 RBS 2136..2723 /label=IRES CDS 2107..2723 /label=BBa_K1993023 misc_feature 2724..2726 /label=start codon CDS 2727..3440 /label=eGFP misc_feature 3441..3443 /label=stop codon misc_feature 2724..3443 /label=BBa_K1993017 ORIGIN 1 atgaactacc cgctaacgct ggaaatggac ctcgagaacc tggaggacct gttctgggaa 61 ctggacagat tggacaacta taacgacacc tccctggtgg aaaatcatct ctgccctgcc 121 acagaggggc ccctcatggc ctccttcaag gccgtgttcg tgcccgtggc ctacagcctc 181 atcttcctcc tgggcgtgat cggcaacgtc ctggtgctgg tgatcctgga gcggcaccgg 241 cagacacgca gttccacgga gaccttcctg ttccacctgg ccgtggccga cctcctgctg 301 gtcttcatct tgccctttgc cgtggccgag ggctctgtgg gctgggtcct ggggaccttc 361 ctctgcaaaa ctgtgattgc cctgcacaaa gtcaacttct actgtagcag cctgctcctg 421 gcctgcatcg ccgtggaccg ctacctggcc attgtccacg ccgtccatgc ctaccgccac 481 cgccgcctcc tctccatcca catcacctgt gggaccatct ggctggtggg cttcctcctt 541 gccttgccag agattctctt cgccaaagtc agccaaggcc atcacaacaa ctccctgcca 601 cgttgcacct tctcccaaga gaaccaagca gaaacgcatg cctggttcac ctcccgattc 661 ctctaccatg tggcgggatt cctgctgccc atgctggtga tgggctggtg ctacgtgggg 721 gtagtgcaca ggttgcgcca ggcccagcgg cgccctcagc ggcagaaggc agtcagggtg 781 gccatcctgg tgacaagcat cttcttcctc tgctggtcac cctaccacat cgtcatcttc 841 ctggacaccc tggcgaggct gaaggccgtg gacaatacct gcaagctgaa tggctctctc 901 cccgtggcca tcaccatgtg tgagttcctg ggcctggccc actgctgcct caaccccatg 961 ctctacactt tcgccggcgt gaagttccgc agtgacctgt cgcggctcct gacgaagctg 1021 ggctgtaccg gccctgcctc cctgtgccag ctcttcccta gctggcgcag gagcagtctc 1081 tctgagtcag agaatgccac ctctctcacc acgttcgagg gcaggggaag tcttctaaca 1141 tgcggggacg tggaggaaaa tcccggcccc atgacttcga aagtttatga tccagaacaa 1201 aggaaacgga tgataactgg tccgcagtgg tgggccagat gtaaacaaat gaatgttctt 1261 gattcattta ttaattatta tgattcagaa aaacatgcag aaaatgctgt tattttttta 1321 catggtaacg cggcctcttc ttatttatgg cgacatgttg tgccacatat tgagccagta 1381 gcgcggtgta ttataccaga tcttattggt atgggcaaat caggcaaatc tggtaatggt 1441 tcttataggt tacttgatca ttacaaatat cttactgcat ggtttgaact tcttaattta 1501 ccaaagaaga tcatttttgt cggccatgat tggggtgctt gtttggcatt tcattatagc 1561 tatgagcatc aagataagat caaagcaata gttcacgctg aaagtgtagt agatgtgatt 1621 gaatcatggg atgaatggcc tgatattgaa gaagatattg cgttgatcaa atctgaagaa 1681 ggagaaaaaa tggttttgga gaataacttc ttcgtggaaa ccatgttgcc atcaaaaatc 1741 atgagaaagt tagaaccaga agaatttgca gcatatcttg aaccattcaa agagaaaggt 1801 gaagttcgtc gtccaacatt atcatggcct cgtgaaatcc cgttagtaaa aggtggtaaa 1861 cctgacgttg tacaaattgt taggaattat aatgcttatc tacgtgcaag tgatgattta 1921 ccaaaaatgt ttattgaatc ggatccagga ttcttttcca atgctattgt tgaaggcgcc 1981 aagaagtttc ctaatactga atttgtcaaa gtaaaaggtc ttcatttttc gcaagaagat 2041 gcacctgatg aaatgggaaa atatatcaaa tcgttcgttg agcgagttct caaaaatgaa 2101 caataagggg accactttgt acaagaaagc tgggtgcccc tctccctccc ccccccctaa 2161 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc 2221 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac 2281 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt 2341 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg 2401 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata 2461 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga 2521 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt 2581 accccattgt atgggatctg atctggggcc tcggtacaca tgctttacat gtgtttagtc 2641 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac 2701 acgatgataa tatggccaca accatggtga gcaagggcga ggagctgttc accggggtgg 2761 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg 2821 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca 2881 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca 2941 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct 3001 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg 3061 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg 3121 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata 3181 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg 3241 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc 3301 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca 3361 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg 3421 gcatggacga gctgtacaag taa //