LOCUS       BBa_K1993026            3012 bp    DNA     linear       05-Nov-2024
DEFINITION  Luciferase-T2A-dtomato-T2A-hFTH
ACCESSION   BBa_K1993026
VERSION     BBa_K1993026.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=start codon
     CDS             4..1650
                     /label=Luciferase
     misc_feature    1651..1704
                     /label=T2A
     misc_feature    1705..1707
                     /label=start codon
     CDS             1708..2406
                     /label=dTomato
     misc_feature    2407..2460
                     /label=T2A
     misc_feature    2461..2463
                     /label=start codon for hFTH
     CDS             2464..3009
                     /label=hFTH
     misc_feature    3010..3012
                     /label=stop codon for hFTH
ORIGIN
        1 atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct agaggatgga
       61 accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt
      121 gcttttacag atgcacatat cgaggtgaac atcacgtacg cggaatactt cgaaatgtcc
      181 gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta
      241 tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt
      301 gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgaacatt
      361 tcgcagccta ccgtagtgtt tgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa
      421 aaaaaattac caataatcca gaaaattatt atcatggatt ctaaaacgga ttaccaggga
      481 tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat
      541 tttgtaccag agtcctttga tcgtgacaaa acaattgcac tgataatgaa ctcctctgga
      601 tctactgggt tacctaaggg tgtggccctt ccgcatagaa ctgcctgcgt cagattctcg
      661 catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt
      721 gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt
      781 cgagtcgtct taatgtatag atttgaagaa gagctgtttt tacgatccct tcaggattac
      841 aaaattcaaa gtgcgttgct agtaccaacc ctattttcat tcttcgccaa aagcactctg
      901 attgacaaat acgatttatc taatttacac gaaattgctt ctgggggcgc acctctttcg
      961 aaagaagtcg gggaagcggt tgcaaaacgc ttccatcttc cagggatacg acaaggatat
     1021 gggctcactg agactacatc agctattctg attacacccg agggggatga taaaccgggc
     1081 gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa
     1141 acgctgggcg ttaatcagag aggcgaatta tgtgtcagag gacctatgat tatgtccggt
     1201 tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct
     1261 ggagacatag cttactggga cgaagacgaa cacttcttca tagttgaccg cttgaagtct
     1321 ttaattaaat acaaaggata ccaggtggcc cccgctgaat tggagtcgat attgttacaa
     1381 caccccaaca tcttcgacgc gggcgtggca ggtcttcccg acgatgacgc cggtgaactt
     1441 cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat
     1501 tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac
     1561 gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata
     1621 aaggccaaga agggcggaaa gtccaaattg gagggcaggg gaagtcttct aacatgcggg
     1681 gacgtggagg aaaatcccgg ccccatggtg agcaagggcg aggaggtcat caaagagttc
     1741 atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc
     1801 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc
     1861 ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg
     1921 tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc
     1981 aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc
     2041 tccctacagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc
     2101 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac
     2161 ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc
     2221 cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc
     2281 tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg
     2341 gaacagtacg agcgctccga gggccgccac cacctgttcc tgtacggcat ggacgagctg
     2401 tacaaggagg gcaggggaag tcttctaaca tgcggggacg tggaggaaaa tcccggcccc
     2461 atgacgaccg cgtccacctc gcaggtgcgc cagaactacc accaggactc agaggccgcc
     2521 atcaaccgcc agatcaacct ggagctctac gcctcctacg tttacctgtc catgtcttac
     2581 tactttgacc gcgatgatgt ggctttgaag aactttgcca aatactttct tcaccaatct
     2641 catgaggaga gggaacatgc tgagaaactg atgaagctac agaaccaacg aggtggccga
     2701 atcttccttc aggatatcaa gaaaccagac tgtgatgact gggagagcgg gctgaatgca
     2761 atggagtgtg cattacattt ggaaaaaaat gtgaatcagt cactactgga actgcacaaa
     2821 ctggccactg acaaaaatga cccccatttg tgtgacttca ttgagacaca ttacctgaat
     2881 gagcaggtga aagccatcaa agaattgggt gaccacgtga ccaacttgcg caagatggga
     2941 gcgcccgaat ctggcttggc ggaatatctc tttgacaagc acaccctggg agacagtgat
     3001 aatgaaagct ga
//