LOCUS       BBa_K1993027            2408 bp    DNA     linear       09-Jan-2025
DEFINITION  CXCR4-IRES-eGFP
ACCESSION   BBa_K1993027
VERSION     BBa_K1993027.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=start codon
     CDS             4..1068
                     /label=CXCR4
     misc_feature    1069..1071
                     /label=stop codon
     RBS             1101..1688
                     /label=IRES
     misc_feature    1072..1100
                     /label=attB2
     misc_feature    1689..1691
                     /label=start codon for eGFP
     CDS             1692..2405
                     /label=eGFP
     misc_feature    2406..2408
                     /label=stop codon for eGFP
ORIGIN
        1 atgtccattc ctttgcctct tttgcagata tacacttcag ataactacac cgaggaaatg
       61 ggctcagggg actatgactc catgaaggaa ccctgtttcc gtgaagaaaa tgctaatttc
      121 aataaaatct tcctgcccac catctactcc atcatcttct taactggcat tgtgggcaat
      181 ggattggtca tcctggtcat gggttaccag aagaaactga gaagcatgac ggacaagtac
      241 aggctgcacc tgtcagtggc cgacctcctc tttgtcatca cgcttccctt ctgggcagtt
      301 gatgccgtgg caaactggta ctttgggaac ttcctatgca aggcagtcca tgtcatctac
      361 acagtcaacc tctacagcag tatcctcatc ctggccttca tcagtctgga ccgctacctg
      421 gccatcgtcc acgccaccaa cagtcagagg ccaaggaagc tgttggctga agaggtggtc
      481 tatgttggcg tctggatccc tgccctcctg ccgactattc ccgacttcat ctttgccaac
      541 gtcagtgagg cagatgacag atatatctgt gaccgcttct accccaatga cttgtgggtg
      601 gttgtgttcc agtttcagca catcatggtt ggccttatcc tgcctggtat tgtcatcctg
      661 tcctgctatt gcattatcat ctccaagctg tcacactcca agggccacca gaagcgcaag
      721 gccctcaaga ccacagtcat cctcatcctg gctttcttcg cctgttggct gccttactac
      781 attgggatca gcatcgactc cttcatcctc ctggaaatca tcaagcaagg gtgtgagttt
      841 gagaacactg tgcacaagtg gatttccatc accgaggccc tagctttctt ccactgttgt
      901 ctgaacccca tcctctatgc tttccttgga gccaaattta aaacctctgc ccagcacgca
      961 ctcacctctg tgagcagagg gtccagcctc aagatcctct ccaaaggaaa gcgaggtgga
     1021 cattcatctg tttccactga gtctgagtct tcaagttttc actccagcta aggggaccac
     1081 tttgtacaag aaagctgggt gcccctctcc ctcccccccc cctaacgtta ctggccgaag
     1141 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     1201 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     1261 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     1321 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     1381 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     1441 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     1501 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     1561 atctgatctg gggcctcggt acacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     1621 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg
     1681 ccacaaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     1741 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     1801 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     1861 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     1921 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     1981 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     2041 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     2101 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     2161 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     2221 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     2281 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     2341 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     2401 acaagtga
//