LOCUS BBa_K1993031 4700 bp DNA linear 31-Oct-2024 DEFINITION CXCR4-IRES-Luciferase-T2A-dtomato-T2A-hFTH ACCESSION BBa_K1993031 VERSION BBa_K1993031.1 FEATURES Location/Qualifiers misc_feature 1..3 /label=start codon for CXCR4 CDS 4..1068 /label=CXCR4 misc_feature 1069..1071 /label=stop codon for CXCR4 misc_feature 1072..1100 /label=attB2 RBS 1101..1688 /label=IRES misc_feature 1689..1691 /label=start codon for Luciferase CDS 1692..3335 /label=Luciferase misc_feature 3336..3389 /label=T2A misc_feature 3390..3392 /label=start codon for dTomato CDS 3393..4094 /label=dTomato misc_feature 4095..4148 /label=T2A misc_feature 4149..4151 /label=start codon for hFTH CDS 4152..4697 /label=hFTH misc_feature 4698..4700 /label=stopp codon for hFTH ORIGIN 1 atgtccattc ctttgcctct tttgcagata tacacttcag ataactacac cgaggaaatg 61 ggctcagggg actatgactc catgaaggaa ccctgtttcc gtgaagaaaa tgctaatttc 121 aataaaatct tcctgcccac catctactcc atcatcttct taactggcat tgtgggcaat 181 ggattggtca tcctggtcat gggttaccag aagaaactga gaagcatgac ggacaagtac 241 aggctgcacc tgtcagtggc cgacctcctc tttgtcatca cgcttccctt ctgggcagtt 301 gatgccgtgg caaactggta ctttgggaac ttcctatgca aggcagtcca tgtcatctac 361 acagtcaacc tctacagcag tatcctcatc ctggccttca tcagtctgga ccgctacctg 421 gccatcgtcc acgccaccaa cagtcagagg ccaaggaagc tgttggctga agaggtggtc 481 tatgttggcg tctggatccc tgccctcctg ccgactattc ccgacttcat ctttgccaac 541 gtcagtgagg cagatgacag atatatctgt gaccgcttct accccaatga cttgtgggtg 601 gttgtgttcc agtttcagca catcatggtt ggccttatcc tgcctggtat tgtcatcctg 661 tcctgctatt gcattatcat ctccaagctg tcacactcca agggccacca gaagcgcaag 721 gccctcaaga ccacagtcat cctcatcctg gctttcttcg cctgttggct gccttactac 781 attgggatca gcatcgactc cttcatcctc ctggaaatca tcaagcaagg gtgtgagttt 841 gagaacactg tgcacaagtg gatttccatc accgaggccc tagctttctt ccactgttgt 901 ctgaacccca tcctctatgc tttccttgga gccaaattta aaacctctgc ccagcacgca 961 ctcacctctg tgagcagagg gtccagcctc aagatcctct ccaaaggaaa gcgaggtgga 1021 cattcatctg tttccactga gtctgagtct tcaagttttc actccagcta aggggaccac 1081 tttgtacaag aaagctgggt gcccctctcc ctcccccccc cctaacgtta ctggccgaag 1141 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc 1201 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg 1261 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc 1321 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc 1381 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa 1441 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct 1501 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg 1561 atctgatctg gggcctcggt acacatgctt tacatgtgtt tagtcgaggt taaaaaaacg 1621 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg 1681 ccacaaccat ggaagacgcc aaaaacataa agaaaggccc ggcgccattc tatccgctag 1741 aggatggaac cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg 1801 gaacaattgc ttttacagat gcacatatcg aggtgaacat cacgtacgcg gaatacttcg 1861 aaatgtccgt tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa 1921 tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta 1981 tcggagttgc agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta 2041 tgaacatttc gcagcctacc gtagtgtttg tttccaaaaa ggggttgcaa aaaattttga 2101 acgtgcaaaa aaaattacca ataatccaga aaattattat catggattct aaaacggatt 2161 accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg 2221 aatacgattt tgtaccagag tcctttgatc gtgacaaaac aattgcactg ataatgaact 2281 cctctggatc tactgggtta cctaagggtg tggcccttcc gcatagaact gcctgcgtca 2341 gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt 2401 taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat 2461 gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgttttta cgatcccttc 2521 aggattacaa aattcaaagt gcgttgctag taccaaccct attttcattc ttcgccaaaa 2581 gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct gggggcgcac 2641 ctctttcgaa agaagtcggg gaagcggttg caaaacgctt ccatcttcca gggatacgac 2701 aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag ggggatgata 2761 aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata 2821 ccgggaaaac gctgggcgtt aatcagagag gcgaattatg tgtcagagga cctatgatta 2881 tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc 2941 tacattctgg agacatagct tactgggacg aagacgaaca cttcttcata gttgaccgct 3001 tgaagtcttt aattaaatac aaaggatacc aggtggcccc cgctgaattg gagtcgatat 3061 tgttacaaca ccccaacatc ttcgacgcgg gcgtggcagg tcttcccgac gatgacgccg 3121 gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga 3181 tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt 3241 ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga 3301 tcctcataaa ggccaagaag ggcggaaagt ccaaattgga gggcagggga agtcttctaa 3361 catgcgggga cgtggaggaa aatcccggcc ccatggtgag caagggcgag gaggtcatca 3421 aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga 3481 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga 3541 ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct 3601 ccaaggcgta cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg 3661 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc 3721 aggactcctc cctacaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact 3781 tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc 3841 gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg 3901 acggcggcca ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac 3961 tgcccggcta ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca 4021 ccatcgtgga acagtacgag cgctccgagg gccgccacca cctgttcctg tacggcatgg 4081 acgagctgta caaggagggc aggggaagtc ttctaacatg cggggacgtg gaggaaaatc 4141 ccggccccat gacgaccgcg tccacctcgc aggtgcgcca gaactaccac caggactcag 4201 aggccgccat caaccgccag atcaacctgg agctctacgc ctcctacgtt tacctgtcca 4261 tgtcttacta ctttgaccgc gatgatgtgg ctttgaagaa ctttgccaaa tactttcttc 4321 accaatctca tgaggagagg gaacatgctg agaaactgat gaagctacag aaccaacgag 4381 gtggccgaat cttccttcag gatatcaaga aaccagactg tgatgactgg gagagcgggc 4441 tgaatgcaat ggagtgtgca ttacatttgg aaaaaaatgt gaatcagtca ctactggaac 4501 tgcacaaact ggccactgac aaaaatgacc cccatttgtg tgacttcatt gagacacatt 4561 acctgaatga gcaggtgaaa gccatcaaag aattgggtga ccacgtgacc aacttgcgca 4621 agatgggagc gcccgaatct ggcttggcgg aatatctctt tgacaagcac accctgggag 4681 acagtgataa tgaaagctga //