LOCUS BBa_K1993035 3600 bp DNA linear 22-Nov-2024 DEFINITION IRES-Luciferase-T2A-dtomato-T2A-hFTH ACCESSION BBa_K1993035 VERSION BBa_K1993035.1 FEATURES Location/Qualifiers RBS 1..588 /label=IRES RBS 1..588 /label=BBa_K1993016 misc_feature 589..591 /label=start codon CDS 592..2235 /label=Luciferase(Firefly Luciferase) misc_feature 2236..2238 /label=stop codon misc_feature 589..2238 /label=BBa_K1993018 misc_feature 2239..2292 /label=T2A misc_feature 2239..2292 /label=BBa_K1993019 misc_feature 2293..2295 /label=start codon CDS 2296..2991 /label=dTomato misc_feature 2992..2994 /label=stop codon misc_feature 2293..2994 /label=BBa_K1993020 misc_feature 2995..3048 /label=T2A misc_feature 2995..3048 /label=BBa_K1993019 misc_feature 3049..3051 /label=start codon CDS 3052..3597 /label=hFTH misc_feature 3598..3600 /label=stop codon misc_feature 3049..3600 /label=BBa_K1993021 ORIGIN 1 gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt 61 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc 121 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag 181 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac 241 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc 301 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc 361 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca 421 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt 481 acacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg 541 gggacgtggt tttcctttga aaaacacgat gataatatgg ccacaaccat ggaagacgcc 601 aaaaacataa agaaaggccc ggcgccattc tatccgctag aggatggaac cgctggagag 661 caactgcata aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat 721 gcacatatcg aggtgaacat cacgtacgcg gaatacttcg aaatgtccgt tcggttggca 781 gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac 841 tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc 901 gcgaacgaca tttataatga acgtgaattg ctcaacagta tgaacatttc gcagcctacc 961 gtagtgtttg tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaaattacca 1021 ataatccaga aaattattat catggattct aaaacggatt accagggatt tcagtcgatg 1081 tacacgttcg tcacatctca tctacctccc ggttttaatg aatacgattt tgtaccagag 1141 tcctttgatc gtgacaaaac aattgcactg ataatgaact cctctggatc tactgggtta 1201 cctaagggtg tggcccttcc gcatagaact gcctgcgtca gattctcgca tgccagagat 1261 cctatttttg gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat 1321 cacggttttg gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta 1381 atgtatagat ttgaagaaga gctgttttta cgatcccttc aggattacaa aattcaaagt 1441 gcgttgctag taccaaccct attttcattc ttcgccaaaa gcactctgat tgacaaatac 1501 gatttatcta atttacacga aattgcttct gggggcgcac ctctttcgaa agaagtcggg 1561 gaagcggttg caaaacgctt ccatcttcca gggatacgac aaggatatgg gctcactgag 1621 actacatcag ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa 1681 gttgttccat tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt 1741 aatcagagag gcgaattatg tgtcagagga cctatgatta tgtccggtta tgtaaacaat 1801 ccggaagcga ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct 1861 tactgggacg aagacgaaca cttcttcata gttgaccgct tgaagtcttt aattaaatac 1921 aaaggatacc aggtggcccc cgctgaattg gagtcgatat tgttacaaca ccccaacatc 1981 ttcgacgcgg gcgtggcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt 2041 gttgttttgg agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt 2101 caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa 2161 ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag 2221 ggcggaaagt ccaaattgga gggcagggga agtcttctaa catgcgggga cgtggaggaa 2281 aatcccggcc ccatggtgag caagggcgag gaggtcatca aagagttcat gcgcttcaag 2341 gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc 2401 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc 2461 ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac 2521 cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc 2581 gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctacaggac 2641 ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta 2701 atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc 2761 gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg 2821 gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg 2881 gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag 2941 cgctccgagg gccgccacca cctgttcctg tacggcatgg acgagctgta caaggagggc 3001 aggggaagtc ttctaacatg cggggacgtg gaggaaaatc ccggccccat gacgaccgcg 3061 tccacctcgc aggtgcgcca gaactaccac caggactcag aggccgccat caaccgccag 3121 atcaacctgg agctctacgc ctcctacgtt tacctgtcca tgtcttacta ctttgaccgc 3181 gatgatgtgg ctttgaagaa ctttgccaaa tactttcttc accaatctca tgaggagagg 3241 gaacatgctg agaaactgat gaagctacag aaccaacgag gtggccgaat cttccttcag 3301 gatatcaaga aaccagactg tgatgactgg gagagcgggc tgaatgcaat ggagtgtgca 3361 ttacatttgg aaaaaaatgt gaatcagtca ctactggaac tgcacaaact ggccactgac 3421 aaaaatgacc cccatttgtg tgacttcatt gagacacatt acctgaatga gcaggtgaaa 3481 gccatcaaag aattgggtga ccacgtgacc aacttgcgca agatgggagc gcccgaatct 3541 ggcttggcgg aatatctctt tgacaagcac accctgggag acagtgataa tgaaagctaa //