LOCUS BBa_K1994000 4104 bp DNA linear 14-Nov-2024 DEFINITION dCas9 enzyme ACCESSION BBa_K1994000 VERSION BBa_K1994000.1 FEATURES Location/Qualifiers ORIGIN 1 gataagaaat actcaatagg cttagctatc ggcacaaata gcgtcggatg ggcggtgatc 61 actgatgaat ataaggttcc gtctaaaaag ttcaaggttc tgggaaatac agaccgccac 121 agtatcaaaa aaaatcttat aggggctctt ttatttgaca gtggagagac agcggaagcg 181 actcgtctca aacggacagc tcgtagaagg tatacacgtc ggaagaatcg tatttgttat 241 ctacaggaga ttttttcaaa tgagatggcg aaagtagatg atagtttctt tcatcgactt 301 gaagagtctt ttttggtgga agaagacaag aagcatgaac gtcatcctat ttttggaaat 361 atagtagatg aagttgctta tcatgagaaa tatccaacta tctatcatct gcgaaaaaaa 421 ttggtagatt ctactgataa agcggatttg cgcttaatct atttggcctt agcgcatatg 481 attaagtttc gtggtcattt tttgattgag ggagatttaa atcctgataa tagtgatgtg 541 gacaaactat ttatccagtt ggtacaaacc tacaatcaat tatttgaaga aaaccctatt 601 aacgcaagtg gagtagatgc taaagcgatt ctttctgcac gattgagtaa atcaagacga 661 ttagaaaatc tcattgctca gctccccggt gagaagaaaa atggcttatt tgggaatctc 721 attgctttgt cattgggttt gacccctaat tttaaatcaa attttgattt ggcagaagat 781 gctaaattac agctttcaaa agatacttac gatgatgatt tagataattt attggcgcaa 841 attggagatc aatatgctga tttgtttttg gcagctaaga atttatcaga tgctatttta 901 ctttcagata tcctaagagt aaatactgaa ataactaagg ctcccctatc agcttcaatg 961 attaaacgct acgatgaaca tcatcaagac ttgactcttt taaaagcttt agttcgacaa 1021 caacttccag aaaagtataa agaaatcttt tttgatcaat caaaaaacgg atatgcaggt 1081 tatattgatg ggggagctag ccaagaagaa ttttataaat ttatcaaacc aattttagaa 1141 aaaatggatg gtactgagga attattggtg aaactaaatc gtgaagattt gctgcgcaag 1201 caacggacct ttgacaacgg ctctattccc catcaaattc acttgggtga gctgcatgct 1261 attttgagaa gacaagaaga cttttatcca tttttaaaag acaatcgtga gaagattgaa 1321 aaaatcttga cttttcgtat tccttattat gttggtccat tggcgcgtgg caatagtcgt 1381 tttgcatgga tgactcggaa gtctgaagaa acaattaccc catggaattt tgaagaagtt 1441 gtcgataaag gtgcttcagc tcaatcattt attgaacgca tgacaaactt tgataaaaat 1501 cttccaaatg aaaaagtact accaaaacat agtttgcttt atgagtattt tacggtttat 1561 aacgaattga caaaggtcaa atatgttact gaaggaatgc gaaaaccagc atttctttca 1621 ggtgaacaga agaaagccat tgttgattta ctcttcaaaa caaatcgaaa agtaaccgtt 1681 aagcaattaa aagaagatta tttcaaaaaa atagaatgtt ttgatagtgt tgaaatttca 1741 ggagttgaag atagatttaa tgcttcatta ggtacctacc atgatttgct aaaaattatt 1801 aaagataaag attttttgga taatgaagaa aatgaagata tcttagagga tattgtttta 1861 acattgacct tatttgaaga tagggagatg attgaggaaa gacttaaaac atatgctcac 1921 ctctttgatg ataaggtgat gaaacagctt aaacgtcgcc gttatactgg ttggggacgt 1981 ttgtctcgaa aattgattaa tggtattagg gataagcaat ctggcaaaac aatattagat 2041 tttttgaaat cagatggttt tgccaatcgc aattttatgc agctgatcca tgatgatagt 2101 ttgacattta aagaagacat tcaaaaagca caagtgtctg gacaaggcga tagtttacat 2161 gaacatattg caaatttagc tggtagccct gctattaaaa aaggtatttt acagactgta 2221 aaagttgttg atgaattggt caaagtaatg gggcggcata agccagaaaa tatcgttatt 2281 gaaatggcac gtgaaaatca gacaactcaa aagggccaga aaaattcgcg agagcgtatg 2341 aaacgaatcg aagaaggtat caaagaatta ggaagtcaga ttcttaaaga gcatcctgtt 2401 gaaaatactc aattgcaaaa tgaaaagctc tatctctatt atctccaaaa tggaagagac 2461 atgtatgtgg accaagaatt agatattaat cgtttaagtg attatgatgt cgatgccatt 2521 gttccacaaa gtttccttaa agacgattca atagacaata aggtcttaac gcgttctgat 2581 aaaaatcgtg gtaaatcgga taacgttcca agtgaagaag tagtcaaaaa gatgaaaaac 2641 tattggagac aacttctaaa cgccaagtta atcactcaac gtaagtttga taatttaacg 2701 aaagctgaac gtggaggttt gagtgaactt gataaagctg gttttatcaa acgccaattg 2761 gttgaaactc gccaaatcac taagcatgtg gcacaaattt tggatagtcg catgaatact 2821 aaatacgatg aaaatgataa acttattcga gaggttaaag tgattacctt aaaatctaaa 2881 ttagtttctg acttccgaaa agatttccaa ttctataaag tacgtgagat taacaattac 2941 catcatgccc atgatgcgta tctaaatgcc gtcgttggaa ctgctttgat taagaaatat 3001 ccaaaacttg aatcggagtt tgtctatggt gattataaag tttatgatgt tcgtaaaatg 3061 attgctaagt ctgagcaaga aataggcaaa gcaaccgcaa aatatttctt ttactctaat 3121 atcatgaact tcttcaaaac agaaattaca cttgcaaatg gagagattcg caaacgccct 3181 ctaatcgaaa ctaatgggga aactggagaa attgtctggg ataaagggcg agattttgcc 3241 acagtgcgca aagtattgtc catgccccaa gtcaatattg tcaagaaaac agaagtacag 3301 acaggcggat tctccaagga gtcaatttta ccaaaaagaa attcggacaa gcttattgct 3361 cgtaaaaaag actgggatcc aaaaaaatat ggtggttttg atagtccaac ggtagcttat 3421 tcagtcctag tggttgctaa ggtggaaaaa gggaaatcga agaagttaaa atccgttaaa 3481 gagttactag ggatcacaat tatggaaaga agttcctttg aaaaaaatcc gattgacttt 3541 ttagaagcta aaggatataa ggaagttaaa aaagacttaa tcattaaact acctaaatat 3601 agtctttttg agttagaaaa cggtcgtaaa cggatgctgg ctagtgccgg agaattacaa 3661 aaaggaaatg agctggctct gccaagcaaa tatgtgaatt ttttatattt agctagtcat 3721 tatgaaaagt tgaagggtag tccagaagat aacgaacaaa aacaattgtt tgtggagcag 3781 cataagcatt atttagatga gattattgag caaatcagtg aattttctaa gcgtgttatt 3841 ttagcagatg ccaatttaga taaagttctt agtgcatata acaaacatag agacaaacca 3901 atacgtgaac aagcagaaaa tattattcat ttatttacgt tgacgaatct tggagctccc 3961 gctgctttta aatattttga tacaacaatt gatcgtaaac gatatacgtc tacaaaagaa 4021 gttttagatg ccactcttat ccatcaatcc atcactggtc tttatgaaac acgcattgat 4081 ttgagtcagc taggaggtga ctga //