LOCUS       BBa_K1994013             189 bp    DNA     linear       06-Dec-2025
DEFINITION  sgRNA with dCas9 binding site sequence 9 and PP7 handle insert
ACCESSION   BBa_K1994013
VERSION     BBa_K1994013.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=Sequence 9
     stem_loop       21..62
                     /label=dCas9 handle
     stem_loop       63..96
                     /label=truncated S. pyrogenes terminator
     misc_feature    97..107
                     /label=10bp Linker
     stem_loop       150..189
                     /label=aspA terminator
     stem_loop       108..138
                     /label=PP7 BP handle
     misc_feature    139..149
                     /label=10bp Linker
ORIGIN
        1 cggtaagtgt aggtgaaagt gttttagagc tagaaatagc aagttaaaat aaggctagtc
       61 cgttatcaac ttgaaaaagt ggcaccgagt cggtgcaccc aacaaaaaca taaggagttt
      121 atatggaaac ccttatgacc caacaaaaca agaaaaaagg cacgtcatct gacgtgcctt
      181 ttttattta
//