BBa_K1994015
1
BBa_K1994015
sgRNA with 5' golden gate adapter and COM protein binding site
2016-09-25T11:00:00Z
2016-10-19T01:28:04Z
synthetic
For use in CRISPR/Cas9 systems. Contains golden gate adapter at 5' end for inserting target sequence. Contains a COM protein binding site in the middle.
false
false
_2461_
30870
27126
9
false
No special considerations
false
Liam Carroll
annotation2484928
1
20nt golden gate adapter
range2484928
1
1
20
annotation2484929
1
dCas9 handle
range2484929
1
21
62
annotation2484933
1
10bp linker
range2484933
1
126
135
annotation2484930
1
truncated S. pyrogenes terminator
range2484930
1
63
96
annotation2484931
1
10bp linker
range2484931
1
97
106
annotation2484932
1
COM handle
range2484932
1
107
125
annotation2484934
1
aspA terminator
range2484934
1
136
176
BBa_K1994015_sequence
1
agagaccactttcggtctctgttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgcaccaataccactgaatgcctgcgagcatcaccaataccaacaagaaaaaaggcacgtcatctgacgtgccttttttattt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z