LOCUS       BBa_K1994017             178 bp    DNA     linear       22-May-2024
DEFINITION  sgRNA with 5' golden gate adapter and PP7 protein binding site
ACCESSION   BBa_K1994017
VERSION     BBa_K1994017.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=20nt golden gate adapter
     stem_loop       21..62
                     /label=dCas9 handle
     stem_loop       63..96
                     /label=truncated S. pyrogenes terminator
     misc_feature    97..107
                     /label=10bp linker
     stem_loop       108..139
                     /label=PP7 handle
     misc_feature    140..150
                     /label=10bp linker
     stem_loop       150..178
                     /label=aspA terminator
ORIGIN
        1 agagaccact ttcggtctct gttttagagc tagaaatagc aagttaaaat aaggctagtc
       61 cgttatcaac ttgaaaaagt ggcaccgagt cggtgcacaa aaacataagg agtttatatg
      121 gaaaccctta tgacccaaca agaaaaaagg cacgtcatct gacgtgcctt ttttattt
//