BBa_K1994021
1
BBa_K1994021
sgRNA containing two golden gate adapters
2016-10-13T11:00:00Z
2016-10-19T08:03:43Z
Synthetic modification of crRNA and tracrRNA found in S. pyrogenes
sgRNA with Bsa 1 Golden Gate adaptors
false
false
_2461_
30870
30870
9
true
To simplify the traditional gRNA comprised of a crRNA containing a dCas9 handle, and tracrRNA which binds to the PAM sequence in CRISPR, we created a joined sgRNA. As modifying the 3' end of the gRNA does not affect dCas9 binding we have used a deactivated dcas9 terminator and placed golden gate adaptable sites. The two sites are located where the complementary PAM-proximal sequence is located, allowing the distance dCas9 binds from the promoter to be adapted.The other golden gate adaptor in the position after the deactivated terminator so any RNA sequence such as an RNA binding protein handle can be inserted. This region is then followed by a real terminator for the strand.
false
Isobel Holden
annotation2503015
1
dCas9 handle
range2503015
1
21
62
annotation2503056
1
Golden Gate adaptor
range2503056
1
97
116
annotation2502949
1
Golden Gate adaptor
range2502949
1
1
20
annotation2503036
1
deactivated dCas9 terminator
range2503036
1
63
96
annotation2503090
1
aspA terminator
range2503090
1
116
157
BBa_K1994021_sequence
1
agagaccactttcggtctctgttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgctgagaccgtacccggtctctacaagaaaaaaggcacgtcatctgacgtgccttttttattt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z