LOCUS BBa_K1994022 5361 bp DNA linear 28-Jun-2024 DEFINITION Multiple dCas9 binding sites with weak omega promoter upstream of GFP + dCas9 expression cassette ACCESSION BBa_K1994022 VERSION BBa_K1994022.1 FEATURES Location/Qualifiers misc_feature 7..210 /label=Multiple dCas9 binding sites misc_feature 210..239 /label=30nt dCas9 spacer promoter 1..239 /label=BBa_K1994019 misc_feature 248..254 /label=Bgl II misc_feature 305..311 /label=Sal I misc_feature 255..304 /label=Weak Sigma 54 promoter promoter 248..311 /label=BBa_K1994034 misc_feature 321..330 /label=TGG (Shine-Dalgarno sequence) RBS 337..366 /label=RBS 28.4k_NEQ RBSCv1.1 RBS 320..366 /label=BBa_K1994004 misc_feature 373..1095 /label=BBa_K1994003 terminator 1104..1155 /label=BBa_K1994018 misc_feature 1164..1180 /label=Spacer promoter 1181..1211 /label=dCas9 Promoter promoter 1164..1211 /label=BBa_K1994044 RBS 1220..1249 /label=BBa_K1994002 CDS 1258..5361 /label=BBa_K1994000 ORIGIN 1 agagtgggac gaaataggta tgaaacggag tgaagtggag agcgaaggat aaacacggaa 61 atagaaggtg aggtaaggat gaatgaggca aattagggac atatgcggta agtgtaggtg 121 aaagtgggat gataaaggtt gagtacggcg agattaggat atttagggca agtataggta 181 aacgaaggct aagaatggaa tgagagagga tgattatcgt tgttgctagc cggcgtggat 241 actagagaga tctattcacc gcagggataa tcaacactgg cacaattcgc tgcttgtagc 301 tggcagtcga ctactagagc ttggtattat gctagctact agacaattaa tagggaggtt 361 ctcgagtact agatgcgtaa aggcgaagaa ctgtttaccg gtgtggttcc gattctggtg 421 gaactggacg gcgatgttaa tggtcataaa ttcagtgttc gcggcgaagg tgaaggcgat 481 gcgacgaacg gcaaactgac cctgaaattt atctgcacca cgggtaaact gccggtcccg 541 tggccgacgc tggtgaccac gctgacctat ggcgttcaat gttttgcgcg ttacccggat 601 cacatgaaac agcacgactt tttcaaatcg gccatgccgg aaggctatgt gcaggaacgt 661 acgattagct ttaaagacga tggtacgtat aaaacccgcg cggaagtgaa attcgaaggc 721 gataccctgg ttaaccgtat cgaactgaaa ggtatcgatt tcaaagaaga cggcaatatt 781 ctgggtcata aactggaata taacttcaat tcccacaacg tgtacatcac cgcggataaa 841 cagaaaaacg gcattaaagc caatttcaaa atccgccata atgtggaaga tggtagcgtt 901 cagctggccg accactatca gcaaaacacg ccgattggtg atggcccggt cctgctgccg 961 gacaatcact acctgagtac ccagtccgtg ctgtcaaaag atccgaacga aaaacgtgac 1021 cacatggtcc tgctggaatt tgtgacggct gcgggtatca cccacggcat ggacgaactg 1081 tataaaaggc cttaatacta gaggacgaac aataaggcct cccaaatcgg ggggcctttt 1141 ttattgataa caaaatacta gagcctaggc caggcatcaa ttgaaatcat caaactcatt 1201 atggatttaa ttactagagc aagggacatc aggaggaggg aattatagat actagaggat 1261 aagaaatact caataggctt agctatcggc acaaatagcg tcggatgggc ggtgatcact 1321 gatgaatata aggttccgtc taaaaagttc aaggttctgg gaaatacaga ccgccacagt 1381 atcaaaaaaa atcttatagg ggctctttta tttgacagtg gagagacagc ggaagcgact 1441 cgtctcaaac ggacagctcg tagaaggtat acacgtcgga agaatcgtat ttgttatcta 1501 caggagattt tttcaaatga gatggcgaaa gtagatgata gtttctttca tcgacttgaa 1561 gagtcttttt tggtggaaga agacaagaag catgaacgtc atcctatttt tggaaatata 1621 gtagatgaag ttgcttatca tgagaaatat ccaactatct atcatctgcg aaaaaaattg 1681 gtagattcta ctgataaagc ggatttgcgc ttaatctatt tggccttagc gcatatgatt 1741 aagtttcgtg gtcatttttt gattgaggga gatttaaatc ctgataatag tgatgtggac 1801 aaactattta tccagttggt acaaacctac aatcaattat ttgaagaaaa ccctattaac 1861 gcaagtggag tagatgctaa agcgattctt tctgcacgat tgagtaaatc aagacgatta 1921 gaaaatctca ttgctcagct ccccggtgag aagaaaaatg gcttatttgg gaatctcatt 1981 gctttgtcat tgggtttgac ccctaatttt aaatcaaatt ttgatttggc agaagatgct 2041 aaattacagc tttcaaaaga tacttacgat gatgatttag ataatttatt ggcgcaaatt 2101 ggagatcaat atgctgattt gtttttggca gctaagaatt tatcagatgc tattttactt 2161 tcagatatcc taagagtaaa tactgaaata actaaggctc ccctatcagc ttcaatgatt 2221 aaacgctacg atgaacatca tcaagacttg actcttttaa aagctttagt tcgacaacaa 2281 cttccagaaa agtataaaga aatctttttt gatcaatcaa aaaacggata tgcaggttat 2341 attgatgggg gagctagcca agaagaattt tataaattta tcaaaccaat tttagaaaaa 2401 atggatggta ctgaggaatt attggtgaaa ctaaatcgtg aagatttgct gcgcaagcaa 2461 cggacctttg acaacggctc tattccccat caaattcact tgggtgagct gcatgctatt 2521 ttgagaagac aagaagactt ttatccattt ttaaaagaca atcgtgagaa gattgaaaaa 2581 atcttgactt ttcgtattcc ttattatgtt ggtccattgg cgcgtggcaa tagtcgtttt 2641 gcatggatga ctcggaagtc tgaagaaaca attaccccat ggaattttga agaagttgtc 2701 gataaaggtg cttcagctca atcatttatt gaacgcatga caaactttga taaaaatctt 2761 ccaaatgaaa aagtactacc aaaacatagt ttgctttatg agtattttac ggtttataac 2821 gaattgacaa aggtcaaata tgttactgaa ggaatgcgaa aaccagcatt tctttcaggt 2881 gaacagaaga aagccattgt tgatttactc ttcaaaacaa atcgaaaagt aaccgttaag 2941 caattaaaag aagattattt caaaaaaata gaatgttttg atagtgttga aatttcagga 3001 gttgaagata gatttaatgc ttcattaggt acctaccatg atttgctaaa aattattaaa 3061 gataaagatt ttttggataa tgaagaaaat gaagatatct tagaggatat tgttttaaca 3121 ttgaccttat ttgaagatag ggagatgatt gaggaaagac ttaaaacata tgctcacctc 3181 tttgatgata aggtgatgaa acagcttaaa cgtcgccgtt atactggttg gggacgtttg 3241 tctcgaaaat tgattaatgg tattagggat aagcaatctg gcaaaacaat attagatttt 3301 ttgaaatcag atggttttgc caatcgcaat tttatgcagc tgatccatga tgatagtttg 3361 acatttaaag aagacattca aaaagcacaa gtgtctggac aaggcgatag tttacatgaa 3421 catattgcaa atttagctgg tagccctgct attaaaaaag gtattttaca gactgtaaaa 3481 gttgttgatg aattggtcaa agtaatgggg cggcataagc cagaaaatat cgttattgaa 3541 atggcacgtg aaaatcagac aactcaaaag ggccagaaaa attcgcgaga gcgtatgaaa 3601 cgaatcgaag aaggtatcaa agaattagga agtcagattc ttaaagagca tcctgttgaa 3661 aatactcaat tgcaaaatga aaagctctat ctctattatc tccaaaatgg aagagacatg 3721 tatgtggacc aagaattaga tattaatcgt ttaagtgatt atgatgtcga tgccattgtt 3781 ccacaaagtt tccttaaaga cgattcaata gacaataagg tcttaacgcg ttctgataaa 3841 aatcgtggta aatcggataa cgttccaagt gaagaagtag tcaaaaagat gaaaaactat 3901 tggagacaac ttctaaacgc caagttaatc actcaacgta agtttgataa tttaacgaaa 3961 gctgaacgtg gaggtttgag tgaacttgat aaagctggtt ttatcaaacg ccaattggtt 4021 gaaactcgcc aaatcactaa gcatgtggca caaattttgg atagtcgcat gaatactaaa 4081 tacgatgaaa atgataaact tattcgagag gttaaagtga ttaccttaaa atctaaatta 4141 gtttctgact tccgaaaaga tttccaattc tataaagtac gtgagattaa caattaccat 4201 catgcccatg atgcgtatct aaatgccgtc gttggaactg ctttgattaa gaaatatcca 4261 aaacttgaat cggagtttgt ctatggtgat tataaagttt atgatgttcg taaaatgatt 4321 gctaagtctg agcaagaaat aggcaaagca accgcaaaat atttctttta ctctaatatc 4381 atgaacttct tcaaaacaga aattacactt gcaaatggag agattcgcaa acgccctcta 4441 atcgaaacta atggggaaac tggagaaatt gtctgggata aagggcgaga ttttgccaca 4501 gtgcgcaaag tattgtccat gccccaagtc aatattgtca agaaaacaga agtacagaca 4561 ggcggattct ccaaggagtc aattttacca aaaagaaatt cggacaagct tattgctcgt 4621 aaaaaagact gggatccaaa aaaatatggt ggttttgata gtccaacggt agcttattca 4681 gtcctagtgg ttgctaaggt ggaaaaaggg aaatcgaaga agttaaaatc cgttaaagag 4741 ttactaggga tcacaattat ggaaagaagt tcctttgaaa aaaatccgat tgacttttta 4801 gaagctaaag gatataagga agttaaaaaa gacttaatca ttaaactacc taaatatagt 4861 ctttttgagt tagaaaacgg tcgtaaacgg atgctggcta gtgccggaga attacaaaaa 4921 ggaaatgagc tggctctgcc aagcaaatat gtgaattttt tatatttagc tagtcattat 4981 gaaaagttga agggtagtcc agaagataac gaacaaaaac aattgtttgt ggagcagcat 5041 aagcattatt tagatgagat tattgagcaa atcagtgaat tttctaagcg tgttatttta 5101 gcagatgcca atttagataa agttcttagt gcatataaca aacatagaga caaaccaata 5161 cgtgaacaag cagaaaatat tattcattta tttacgttga cgaatcttgg agctcccgct 5221 gcttttaaat attttgatac aacaattgat cgtaaacgat atacgtctac aaaagaagtt 5281 ttagatgcca ctcttatcca tcaatccatc actggtcttt atgaaacacg cattgatttg 5341 agtcagctag gaggtgactg a //