##gff-version 3
##sequence-region BBa_K1994022 1 5361
BBa_K1994022	.	engineered_region	373	1095	.	+	0	ID=BBa_K1994003;Name=BBa_K1994003
BBa_K1994022	.	terminator	1104	1155	.	+	0	ID=BBa_K1994018;Name=BBa_K1994018
BBa_K1994022	.	promoter	248	311	.	+	0	ID=BBa_K1994034;Name=BBa_K1994034
BBa_K1994022	.	sequence_feature	305	311	.	+	0	ID=annotation2522809;Name=Sal I;Parent=BBa_K1994034
BBa_K1994022	.	sequence_feature	255	304	.	+	0	ID=annotation2522833;Name=Weak Sigma 54 promoter;Parent=BBa_K1994034
BBa_K1994022	.	sequence_feature	248	254	.	+	0	ID=annotation2522792;Name=Bgl II;Parent=BBa_K1994034
BBa_K1994022	.	ribosome_entry_site	320	366	.	+	0	ID=BBa_K1994004;Name=BBa_K1994004
BBa_K1994022	.	ribosome_entry_site	337	366	.	+	0	ID=annotation2521776;Name=RBS 28.4k_NEQ RBSCv1.1;Parent=BBa_K1994004
BBa_K1994022	.	sequence_feature	321	330	.	+	0	ID=annotation2521745;Name=TGG (Shine-Dalgarno sequence);Parent=BBa_K1994004
BBa_K1994022	.	ribosome_entry_site	1220	1249	.	+	0	ID=BBa_K1994002;Name=BBa_K1994002
BBa_K1994022	.	promoter	1164	1211	.	+	0	ID=BBa_K1994044;Name=BBa_K1994044
BBa_K1994022	.	sequence_feature	1164	1180	.	+	0	ID=annotation2522272;Name=Spacer;Parent=BBa_K1994044
BBa_K1994022	.	promoter	1181	1211	.	+	0	ID=annotation2522296;Name=dCas9 Promoter;Parent=BBa_K1994044
BBa_K1994022	.	CDS	1258	5361	.	+	0	ID=BBa_K1994000;Name=BBa_K1994000
BBa_K1994022	.	promoter	1	239	.	+	0	ID=BBa_K1994019;Name=BBa_K1994019
BBa_K1994022	.	sequence_feature	7	210	.	+	0	ID=annotation2522793;Name=Multiple dCas9 binding sites ;Parent=BBa_K1994019
BBa_K1994022	.	sequence_feature	210	239	.	+	0	ID=annotation2522794;Name=30nt dCas9 spacer;Parent=BBa_K1994019
>BBa_K1994022
agagtgggacgaaataggtatgaaacggagtgaagtggagagcgaaggataaacacggaaatagaaggtgaggtaagga
tgaatgaggcaaattagggacatatgcggtaagtgtaggtgaaagtgggatgataaaggttgagtacggcgagattagg
atatttagggcaagtataggtaaacgaaggctaagaatggaatgagagaggatgattatcgttgttgctagccggcgtg
gatactagagagatctattcaccgcagggataatcaacactggcacaattcgctgcttgtagctggcagtcgactacta
gagcttggtattatgctagctactagacaattaatagggaggttctcgagtactagatgcgtaaaggcgaagaactgtt
taccggtgtggttccgattctggtggaactggacggcgatgttaatggtcataaattcagtgttcgcggcgaaggtgaa
ggcgatgcgacgaacggcaaactgaccctgaaatttatctgcaccacgggtaaactgccggtcccgtggccgacgctgg
tgaccacgctgacctatggcgttcaatgttttgcgcgttacccggatcacatgaaacagcacgactttttcaaatcggc
catgccggaaggctatgtgcaggaacgtacgattagctttaaagacgatggtacgtataaaacccgcgcggaagtgaaa
ttcgaaggcgataccctggttaaccgtatcgaactgaaaggtatcgatttcaaagaagacggcaatattctgggtcata
aactggaatataacttcaattcccacaacgtgtacatcaccgcggataaacagaaaaacggcattaaagccaatttcaa
aatccgccataatgtggaagatggtagcgttcagctggccgaccactatcagcaaaacacgccgattggtgatggcccg
gtcctgctgccggacaatcactacctgagtacccagtccgtgctgtcaaaagatccgaacgaaaaacgtgaccacatgg
tcctgctggaatttgtgacggctgcgggtatcacccacggcatggacgaactgtataaaaggccttaatactagaggac
gaacaataaggcctcccaaatcggggggccttttttattgataacaaaatactagagcctaggccaggcatcaattgaa
atcatcaaactcattatggatttaattactagagcaagggacatcaggaggagggaattatagatactagaggataaga
aatactcaataggcttagctatcggcacaaatagcgtcggatgggcggtgatcactgatgaatataaggttccgtctaa
aaagttcaaggttctgggaaatacagaccgccacagtatcaaaaaaaatcttataggggctcttttatttgacagtgga
gagacagcggaagcgactcgtctcaaacggacagctcgtagaaggtatacacgtcggaagaatcgtatttgttatctac
aggagattttttcaaatgagatggcgaaagtagatgatagtttctttcatcgacttgaagagtcttttttggtggaaga
agacaagaagcatgaacgtcatcctatttttggaaatatagtagatgaagttgcttatcatgagaaatatccaactatc
tatcatctgcgaaaaaaattggtagattctactgataaagcggatttgcgcttaatctatttggccttagcgcatatga
ttaagtttcgtggtcattttttgattgagggagatttaaatcctgataatagtgatgtggacaaactatttatccagtt
ggtacaaacctacaatcaattatttgaagaaaaccctattaacgcaagtggagtagatgctaaagcgattctttctgca
cgattgagtaaatcaagacgattagaaaatctcattgctcagctccccggtgagaagaaaaatggcttatttgggaatc
tcattgctttgtcattgggtttgacccctaattttaaatcaaattttgatttggcagaagatgctaaattacagctttc
aaaagatacttacgatgatgatttagataatttattggcgcaaattggagatcaatatgctgatttgtttttggcagct
aagaatttatcagatgctattttactttcagatatcctaagagtaaatactgaaataactaaggctcccctatcagctt
caatgattaaacgctacgatgaacatcatcaagacttgactcttttaaaagctttagttcgacaacaacttccagaaaa
gtataaagaaatcttttttgatcaatcaaaaaacggatatgcaggttatattgatgggggagctagccaagaagaattt
tataaatttatcaaaccaattttagaaaaaatggatggtactgaggaattattggtgaaactaaatcgtgaagatttgc
tgcgcaagcaacggacctttgacaacggctctattccccatcaaattcacttgggtgagctgcatgctattttgagaag
acaagaagacttttatccatttttaaaagacaatcgtgagaagattgaaaaaatcttgacttttcgtattccttattat
gttggtccattggcgcgtggcaatagtcgttttgcatggatgactcggaagtctgaagaaacaattaccccatggaatt
ttgaagaagttgtcgataaaggtgcttcagctcaatcatttattgaacgcatgacaaactttgataaaaatcttccaaa
tgaaaaagtactaccaaaacatagtttgctttatgagtattttacggtttataacgaattgacaaaggtcaaatatgtt
actgaaggaatgcgaaaaccagcatttctttcaggtgaacagaagaaagccattgttgatttactcttcaaaacaaatc
gaaaagtaaccgttaagcaattaaaagaagattatttcaaaaaaatagaatgttttgatagtgttgaaatttcaggagt
tgaagatagatttaatgcttcattaggtacctaccatgatttgctaaaaattattaaagataaagattttttggataat
gaagaaaatgaagatatcttagaggatattgttttaacattgaccttatttgaagatagggagatgattgaggaaagac
ttaaaacatatgctcacctctttgatgataaggtgatgaaacagcttaaacgtcgccgttatactggttggggacgttt
gtctcgaaaattgattaatggtattagggataagcaatctggcaaaacaatattagattttttgaaatcagatggtttt
gccaatcgcaattttatgcagctgatccatgatgatagtttgacatttaaagaagacattcaaaaagcacaagtgtctg
gacaaggcgatagtttacatgaacatattgcaaatttagctggtagccctgctattaaaaaaggtattttacagactgt
aaaagttgttgatgaattggtcaaagtaatggggcggcataagccagaaaatatcgttattgaaatggcacgtgaaaat
cagacaactcaaaagggccagaaaaattcgcgagagcgtatgaaacgaatcgaagaaggtatcaaagaattaggaagtc
agattcttaaagagcatcctgttgaaaatactcaattgcaaaatgaaaagctctatctctattatctccaaaatggaag
agacatgtatgtggaccaagaattagatattaatcgtttaagtgattatgatgtcgatgccattgttccacaaagtttc
cttaaagacgattcaatagacaataaggtcttaacgcgttctgataaaaatcgtggtaaatcggataacgttccaagtg
aagaagtagtcaaaaagatgaaaaactattggagacaacttctaaacgccaagttaatcactcaacgtaagtttgataa
tttaacgaaagctgaacgtggaggtttgagtgaacttgataaagctggttttatcaaacgccaattggttgaaactcgc
caaatcactaagcatgtggcacaaattttggatagtcgcatgaatactaaatacgatgaaaatgataaacttattcgag
aggttaaagtgattaccttaaaatctaaattagtttctgacttccgaaaagatttccaattctataaagtacgtgagat
taacaattaccatcatgcccatgatgcgtatctaaatgccgtcgttggaactgctttgattaagaaatatccaaaactt
gaatcggagtttgtctatggtgattataaagtttatgatgttcgtaaaatgattgctaagtctgagcaagaaataggca
aagcaaccgcaaaatatttcttttactctaatatcatgaacttcttcaaaacagaaattacacttgcaaatggagagat
tcgcaaacgccctctaatcgaaactaatggggaaactggagaaattgtctgggataaagggcgagattttgccacagtg
cgcaaagtattgtccatgccccaagtcaatattgtcaagaaaacagaagtacagacaggcggattctccaaggagtcaa
ttttaccaaaaagaaattcggacaagcttattgctcgtaaaaaagactgggatccaaaaaaatatggtggttttgatag
tccaacggtagcttattcagtcctagtggttgctaaggtggaaaaagggaaatcgaagaagttaaaatccgttaaagag
ttactagggatcacaattatggaaagaagttcctttgaaaaaaatccgattgactttttagaagctaaaggatataagg
aagttaaaaaagacttaatcattaaactacctaaatatagtctttttgagttagaaaacggtcgtaaacggatgctggc
tagtgccggagaattacaaaaaggaaatgagctggctctgccaagcaaatatgtgaattttttatatttagctagtcat
tatgaaaagttgaagggtagtccagaagataacgaacaaaaacaattgtttgtggagcagcataagcattatttagatg
agattattgagcaaatcagtgaattttctaagcgtgttattttagcagatgccaatttagataaagttcttagtgcata
taacaaacatagagacaaaccaatacgtgaacaagcagaaaatattattcatttatttacgttgacgaatcttggagct
cccgctgcttttaaatattttgatacaacaattgatcgtaaacgatatacgtctacaaaagaagttttagatgccactc
ttatccatcaatccatcactggtctttatgaaacacgcattgatttgagtcagctaggaggtgactga