BBa_K1994004 1 BBa_K1994004 RBS 28.4k_NEQ RBSCv1.1 2016-09-21T11:00:00Z 2016-10-19T12:05:49Z RBS 28.4k_NEQ RBSCv1.1 RBS used for superfolder GFP in team Warwick 2016's project. false false _2461_ 30870 27126 9 false No special design considerations. false Liam Carroll annotation2521745 1 TGG (Shine-Dalgarno sequence) range2521745 1 2 11 annotation2521776 1 RBS 28.4k_NEQ RBSCv1.1 range2521776 1 18 47 BBa_K1994003 1 BBa_K1994003 Superfolder GFP 2016-09-21T11:00:00Z 2016-09-22T03:14:22Z Synthetic vector pET-T7p(-12T)-SpacerA-GFP-LVA Superfolder GFP. 488nm Excitation, 530nm Emission false false _2461_ 27126 27126 9 false No special design considerations. false Liam Carroll BBa_K1994044 1 BBa_K1994044 dCas9 Promoter 2016-10-13T11:00:00Z 2016-10-19T02:12:18Z sgRNA with PAM-Proximal complementary sequence 5 sgRNA with PAM-Proximal complementary sequence 5 false false _2461_ 30870 28134 9 false sgRNA with PAM-Proximal complementary sequence 5 false Egheosa Ogbomo annotation2522272 1 Spacer range2522272 1 1 17 annotation2522296 1 dCas9 Promoter range2522296 1 18 48 BBa_K1994019 1 BBa_K1994019 Multiple dCas9 binding site sequence 2016-09-25T11:00:00Z 2016-10-19T03:50:49Z Escherichia coli Promoter sequence containing multiple PAMs staggered at regular intervals to provide multiple possible sites for dCas9 binding. false false _2461_ 30870 27126 9 true No special considerations false Liam Carroll annotation2522793 1 Multiple dCas9 binding sites range2522793 1 7 210 annotation2522794 1 30nt dCas9 spacer range2522794 1 210 239 BBa_K1994018 1 BBa_K1994018 E. coli PheA terminator 2016-09-25T11:00:00Z 2016-09-26T08:18:57Z Escherichia coli PheA terminator from E. coli false false _2461_ 27126 27126 9 false No special considerations false Liam Carroll BBa_K1994000 1 BBa_K1994000 dCas9 enzyme 2016-09-21T11:00:00Z 2016-09-22T04:26:59Z Streptococcus Pyrogenes Part containing only dCas9 enzyme with no terminator or promoter. Interacts with sgRNA to bind to specific DNA target. false false _2461_ 27126 27126 9 false No special design considerations. false Liam Carroll BBa_K1994002 1 BBa_K1994002 RBS 173k TIRv1 2016-09-21T11:00:00Z 2016-09-22T04:24:08Z RBS 173k TIRv1 RBS used for dCas9 enzyme in team Warwick 2016's project. false false _2461_ 27126 27126 9 false No special design considerations. false Liam Carroll BBa_K1994034 1 BBa_K1994034 Weakened Omega promoter 2016-10-13T11:00:00Z 2016-10-19T08:11:31Z Weakened omega promoter Weakened omega promoter false false _2461_ 30870 28134 9 false Weakened omega promoter false Isobel Holden annotation2522809 1 Sal I range2522809 1 58 64 annotation2522833 1 Weak Sigma 54 promoter range2522833 1 8 57 annotation2522792 1 Bgl II range2522792 1 1 7 BBa_K1994022 1 BBa_K1994022 Multiple dCas9 binding sites with weak omega promoter upstream of GFP + dCas9 expression cassette 2016-10-13T11:00:00Z 2016-10-19T08:02:59Z dCas9 with Pam staggered sequence and omega promoter dCas9 with Pam staggered sequence and omega promoter false false _2461_ 30870 28134 9 true dCas9 with Pam staggered sequence and omega promoter false Isobel Holden component2522856 1 BBa_K1994004 component2522853 1 BBa_K1994034 component2522849 1 BBa_K1994019 component2522862 1 BBa_K1994002 component2522863 1 BBa_K1994000 component2522861 1 BBa_K1994044 component2522858 1 BBa_K1994018 component2522857 1 BBa_K1994003 annotation2522863 1 BBa_K1994000 range2522863 1 1258 5361 annotation2522862 1 BBa_K1994002 range2522862 1 1220 1249 annotation2522858 1 BBa_K1994018 range2522858 1 1104 1155 annotation2522857 1 BBa_K1994003 range2522857 1 373 1095 annotation2522861 1 BBa_K1994044 range2522861 1 1164 1211 annotation2522849 1 BBa_K1994019 range2522849 1 1 239 annotation2522856 1 BBa_K1994004 range2522856 1 320 366 annotation2522853 1 BBa_K1994034 range2522853 1 248 311 BBa_K1994034_sequence 1 agatctattcaccgcagggataatcaacactggcacaattcgctgcttgtagctggcagtcgac BBa_K1994002_sequence 1 caagggacatcaggaggagggaattataga BBa_K1994004_sequence 1 cttggtattatgctagctactagacaattaatagggaggttctcgag BBa_K1994000_sequence 1 gataagaaatactcaataggcttagctatcggcacaaatagcgtcggatgggcggtgatcactgatgaatataaggttccgtctaaaaagttcaaggttctgggaaatacagaccgccacagtatcaaaaaaaatcttataggggctcttttatttgacagtggagagacagcggaagcgactcgtctcaaacggacagctcgtagaaggtatacacgtcggaagaatcgtatttgttatctacaggagattttttcaaatgagatggcgaaagtagatgatagtttctttcatcgacttgaagagtcttttttggtggaagaagacaagaagcatgaacgtcatcctatttttggaaatatagtagatgaagttgcttatcatgagaaatatccaactatctatcatctgcgaaaaaaattggtagattctactgataaagcggatttgcgcttaatctatttggccttagcgcatatgattaagtttcgtggtcattttttgattgagggagatttaaatcctgataatagtgatgtggacaaactatttatccagttggtacaaacctacaatcaattatttgaagaaaaccctattaacgcaagtggagtagatgctaaagcgattctttctgcacgattgagtaaatcaagacgattagaaaatctcattgctcagctccccggtgagaagaaaaatggcttatttgggaatctcattgctttgtcattgggtttgacccctaattttaaatcaaattttgatttggcagaagatgctaaattacagctttcaaaagatacttacgatgatgatttagataatttattggcgcaaattggagatcaatatgctgatttgtttttggcagctaagaatttatcagatgctattttactttcagatatcctaagagtaaatactgaaataactaaggctcccctatcagcttcaatgattaaacgctacgatgaacatcatcaagacttgactcttttaaaagctttagttcgacaacaacttccagaaaagtataaagaaatcttttttgatcaatcaaaaaacggatatgcaggttatattgatgggggagctagccaagaagaattttataaatttatcaaaccaattttagaaaaaatggatggtactgaggaattattggtgaaactaaatcgtgaagatttgctgcgcaagcaacggacctttgacaacggctctattccccatcaaattcacttgggtgagctgcatgctattttgagaagacaagaagacttttatccatttttaaaagacaatcgtgagaagattgaaaaaatcttgacttttcgtattccttattatgttggtccattggcgcgtggcaatagtcgttttgcatggatgactcggaagtctgaagaaacaattaccccatggaattttgaagaagttgtcgataaaggtgcttcagctcaatcatttattgaacgcatgacaaactttgataaaaatcttccaaatgaaaaagtactaccaaaacatagtttgctttatgagtattttacggtttataacgaattgacaaaggtcaaatatgttactgaaggaatgcgaaaaccagcatttctttcaggtgaacagaagaaagccattgttgatttactcttcaaaacaaatcgaaaagtaaccgttaagcaattaaaagaagattatttcaaaaaaatagaatgttttgatagtgttgaaatttcaggagttgaagatagatttaatgcttcattaggtacctaccatgatttgctaaaaattattaaagataaagattttttggataatgaagaaaatgaagatatcttagaggatattgttttaacattgaccttatttgaagatagggagatgattgaggaaagacttaaaacatatgctcacctctttgatgataaggtgatgaaacagcttaaacgtcgccgttatactggttggggacgtttgtctcgaaaattgattaatggtattagggataagcaatctggcaaaacaatattagattttttgaaatcagatggttttgccaatcgcaattttatgcagctgatccatgatgatagtttgacatttaaagaagacattcaaaaagcacaagtgtctggacaaggcgatagtttacatgaacatattgcaaatttagctggtagccctgctattaaaaaaggtattttacagactgtaaaagttgttgatgaattggtcaaagtaatggggcggcataagccagaaaatatcgttattgaaatggcacgtgaaaatcagacaactcaaaagggccagaaaaattcgcgagagcgtatgaaacgaatcgaagaaggtatcaaagaattaggaagtcagattcttaaagagcatcctgttgaaaatactcaattgcaaaatgaaaagctctatctctattatctccaaaatggaagagacatgtatgtggaccaagaattagatattaatcgtttaagtgattatgatgtcgatgccattgttccacaaagtttccttaaagacgattcaatagacaataaggtcttaacgcgttctgataaaaatcgtggtaaatcggataacgttccaagtgaagaagtagtcaaaaagatgaaaaactattggagacaacttctaaacgccaagttaatcactcaacgtaagtttgataatttaacgaaagctgaacgtggaggtttgagtgaacttgataaagctggttttatcaaacgccaattggttgaaactcgccaaatcactaagcatgtggcacaaattttggatagtcgcatgaatactaaatacgatgaaaatgataaacttattcgagaggttaaagtgattaccttaaaatctaaattagtttctgacttccgaaaagatttccaattctataaagtacgtgagattaacaattaccatcatgcccatgatgcgtatctaaatgccgtcgttggaactgctttgattaagaaatatccaaaacttgaatcggagtttgtctatggtgattataaagtttatgatgttcgtaaaatgattgctaagtctgagcaagaaataggcaaagcaaccgcaaaatatttcttttactctaatatcatgaacttcttcaaaacagaaattacacttgcaaatggagagattcgcaaacgccctctaatcgaaactaatggggaaactggagaaattgtctgggataaagggcgagattttgccacagtgcgcaaagtattgtccatgccccaagtcaatattgtcaagaaaacagaagtacagacaggcggattctccaaggagtcaattttaccaaaaagaaattcggacaagcttattgctcgtaaaaaagactgggatccaaaaaaatatggtggttttgatagtccaacggtagcttattcagtcctagtggttgctaaggtggaaaaagggaaatcgaagaagttaaaatccgttaaagagttactagggatcacaattatggaaagaagttcctttgaaaaaaatccgattgactttttagaagctaaaggatataaggaagttaaaaaagacttaatcattaaactacctaaatatagtctttttgagttagaaaacggtcgtaaacggatgctggctagtgccggagaattacaaaaaggaaatgagctggctctgccaagcaaatatgtgaattttttatatttagctagtcattatgaaaagttgaagggtagtccagaagataacgaacaaaaacaattgtttgtggagcagcataagcattatttagatgagattattgagcaaatcagtgaattttctaagcgtgttattttagcagatgccaatttagataaagttcttagtgcatataacaaacatagagacaaaccaatacgtgaacaagcagaaaatattattcatttatttacgttgacgaatcttggagctcccgctgcttttaaatattttgatacaacaattgatcgtaaacgatatacgtctacaaaagaagttttagatgccactcttatccatcaatccatcactggtctttatgaaacacgcattgatttgagtcagctaggaggtgactga BBa_K1994018_sequence 1 gacgaacaataaggcctcccaaatcggggggccttttttattgataacaaaa BBa_K1994022_sequence 1 agagtgggacgaaataggtatgaaacggagtgaagtggagagcgaaggataaacacggaaatagaaggtgaggtaaggatgaatgaggcaaattagggacatatgcggtaagtgtaggtgaaagtgggatgataaaggttgagtacggcgagattaggatatttagggcaagtataggtaaacgaaggctaagaatggaatgagagaggatgattatcgttgttgctagccggcgtggatactagagagatctattcaccgcagggataatcaacactggcacaattcgctgcttgtagctggcagtcgactactagagcttggtattatgctagctactagacaattaatagggaggttctcgagtactagatgcgtaaaggcgaagaactgtttaccggtgtggttccgattctggtggaactggacggcgatgttaatggtcataaattcagtgttcgcggcgaaggtgaaggcgatgcgacgaacggcaaactgaccctgaaatttatctgcaccacgggtaaactgccggtcccgtggccgacgctggtgaccacgctgacctatggcgttcaatgttttgcgcgttacccggatcacatgaaacagcacgactttttcaaatcggccatgccggaaggctatgtgcaggaacgtacgattagctttaaagacgatggtacgtataaaacccgcgcggaagtgaaattcgaaggcgataccctggttaaccgtatcgaactgaaaggtatcgatttcaaagaagacggcaatattctgggtcataaactggaatataacttcaattcccacaacgtgtacatcaccgcggataaacagaaaaacggcattaaagccaatttcaaaatccgccataatgtggaagatggtagcgttcagctggccgaccactatcagcaaaacacgccgattggtgatggcccggtcctgctgccggacaatcactacctgagtacccagtccgtgctgtcaaaagatccgaacgaaaaacgtgaccacatggtcctgctggaatttgtgacggctgcgggtatcacccacggcatggacgaactgtataaaaggccttaatactagaggacgaacaataaggcctcccaaatcggggggccttttttattgataacaaaatactagagcctaggccaggcatcaattgaaatcatcaaactcattatggatttaattactagagcaagggacatcaggaggagggaattatagatactagaggataagaaatactcaataggcttagctatcggcacaaatagcgtcggatgggcggtgatcactgatgaatataaggttccgtctaaaaagttcaaggttctgggaaatacagaccgccacagtatcaaaaaaaatcttataggggctcttttatttgacagtggagagacagcggaagcgactcgtctcaaacggacagctcgtagaaggtatacacgtcggaagaatcgtatttgttatctacaggagattttttcaaatgagatggcgaaagtagatgatagtttctttcatcgacttgaagagtcttttttggtggaagaagacaagaagcatgaacgtcatcctatttttggaaatatagtagatgaagttgcttatcatgagaaatatccaactatctatcatctgcgaaaaaaattggtagattctactgataaagcggatttgcgcttaatctatttggccttagcgcatatgattaagtttcgtggtcattttttgattgagggagatttaaatcctgataatagtgatgtggacaaactatttatccagttggtacaaacctacaatcaattatttgaagaaaaccctattaacgcaagtggagtagatgctaaagcgattctttctgcacgattgagtaaatcaagacgattagaaaatctcattgctcagctccccggtgagaagaaaaatggcttatttgggaatctcattgctttgtcattgggtttgacccctaattttaaatcaaattttgatttggcagaagatgctaaattacagctttcaaaagatacttacgatgatgatttagataatttattggcgcaaattggagatcaatatgctgatttgtttttggcagctaagaatttatcagatgctattttactttcagatatcctaagagtaaatactgaaataactaaggctcccctatcagcttcaatgattaaacgctacgatgaacatcatcaagacttgactcttttaaaagctttagttcgacaacaacttccagaaaagtataaagaaatcttttttgatcaatcaaaaaacggatatgcaggttatattgatgggggagctagccaagaagaattttataaatttatcaaaccaattttagaaaaaatggatggtactgaggaattattggtgaaactaaatcgtgaagatttgctgcgcaagcaacggacctttgacaacggctctattccccatcaaattcacttgggtgagctgcatgctattttgagaagacaagaagacttttatccatttttaaaagacaatcgtgagaagattgaaaaaatcttgacttttcgtattccttattatgttggtccattggcgcgtggcaatagtcgttttgcatggatgactcggaagtctgaagaaacaattaccccatggaattttgaagaagttgtcgataaaggtgcttcagctcaatcatttattgaacgcatgacaaactttgataaaaatcttccaaatgaaaaagtactaccaaaacatagtttgctttatgagtattttacggtttataacgaattgacaaaggtcaaatatgttactgaaggaatgcgaaaaccagcatttctttcaggtgaacagaagaaagccattgttgatttactcttcaaaacaaatcgaaaagtaaccgttaagcaattaaaagaagattatttcaaaaaaatagaatgttttgatagtgttgaaatttcaggagttgaagatagatttaatgcttcattaggtacctaccatgatttgctaaaaattattaaagataaagattttttggataatgaagaaaatgaagatatcttagaggatattgttttaacattgaccttatttgaagatagggagatgattgaggaaagacttaaaacatatgctcacctctttgatgataaggtgatgaaacagcttaaacgtcgccgttatactggttggggacgtttgtctcgaaaattgattaatggtattagggataagcaatctggcaaaacaatattagattttttgaaatcagatggttttgccaatcgcaattttatgcagctgatccatgatgatagtttgacatttaaagaagacattcaaaaagcacaagtgtctggacaaggcgatagtttacatgaacatattgcaaatttagctggtagccctgctattaaaaaaggtattttacagactgtaaaagttgttgatgaattggtcaaagtaatggggcggcataagccagaaaatatcgttattgaaatggcacgtgaaaatcagacaactcaaaagggccagaaaaattcgcgagagcgtatgaaacgaatcgaagaaggtatcaaagaattaggaagtcagattcttaaagagcatcctgttgaaaatactcaattgcaaaatgaaaagctctatctctattatctccaaaatggaagagacatgtatgtggaccaagaattagatattaatcgtttaagtgattatgatgtcgatgccattgttccacaaagtttccttaaagacgattcaatagacaataaggtcttaacgcgttctgataaaaatcgtggtaaatcggataacgttccaagtgaagaagtagtcaaaaagatgaaaaactattggagacaacttctaaacgccaagttaatcactcaacgtaagtttgataatttaacgaaagctgaacgtggaggtttgagtgaacttgataaagctggttttatcaaacgccaattggttgaaactcgccaaatcactaagcatgtggcacaaattttggatagtcgcatgaatactaaatacgatgaaaatgataaacttattcgagaggttaaagtgattaccttaaaatctaaattagtttctgacttccgaaaagatttccaattctataaagtacgtgagattaacaattaccatcatgcccatgatgcgtatctaaatgccgtcgttggaactgctttgattaagaaatatccaaaacttgaatcggagtttgtctatggtgattataaagtttatgatgttcgtaaaatgattgctaagtctgagcaagaaataggcaaagcaaccgcaaaatatttcttttactctaatatcatgaacttcttcaaaacagaaattacacttgcaaatggagagattcgcaaacgccctctaatcgaaactaatggggaaactggagaaattgtctgggataaagggcgagattttgccacagtgcgcaaagtattgtccatgccccaagtcaatattgtcaagaaaacagaagtacagacaggcggattctccaaggagtcaattttaccaaaaagaaattcggacaagcttattgctcgtaaaaaagactgggatccaaaaaaatatggtggttttgatagtccaacggtagcttattcagtcctagtggttgctaaggtggaaaaagggaaatcgaagaagttaaaatccgttaaagagttactagggatcacaattatggaaagaagttcctttgaaaaaaatccgattgactttttagaagctaaaggatataaggaagttaaaaaagacttaatcattaaactacctaaatatagtctttttgagttagaaaacggtcgtaaacggatgctggctagtgccggagaattacaaaaaggaaatgagctggctctgccaagcaaatatgtgaattttttatatttagctagtcattatgaaaagttgaagggtagtccagaagataacgaacaaaaacaattgtttgtggagcagcataagcattatttagatgagattattgagcaaatcagtgaattttctaagcgtgttattttagcagatgccaatttagataaagttcttagtgcatataacaaacatagagacaaaccaatacgtgaacaagcagaaaatattattcatttatttacgttgacgaatcttggagctcccgctgcttttaaatattttgatacaacaattgatcgtaaacgatatacgtctacaaaagaagttttagatgccactcttatccatcaatccatcactggtctttatgaaacacgcattgatttgagtcagctaggaggtgactga BBa_K1994019_sequence 1 agagtgggacgaaataggtatgaaacggagtgaagtggagagcgaaggataaacacggaaatagaaggtgaggtaaggatgaatgaggcaaattagggacatatgcggtaagtgtaggtgaaagtgggatgataaaggttgagtacggcgagattaggatatttagggcaagtataggtaaacgaaggctaagaatggaatgagagaggatgattatcgttgttgctagccggcgtgga BBa_K1994003_sequence 1 atgcgtaaaggcgaagaactgtttaccggtgtggttccgattctggtggaactggacggcgatgttaatggtcataaattcagtgttcgcggcgaaggtgaaggcgatgcgacgaacggcaaactgaccctgaaatttatctgcaccacgggtaaactgccggtcccgtggccgacgctggtgaccacgctgacctatggcgttcaatgttttgcgcgttacccggatcacatgaaacagcacgactttttcaaatcggccatgccggaaggctatgtgcaggaacgtacgattagctttaaagacgatggtacgtataaaacccgcgcggaagtgaaattcgaaggcgataccctggttaaccgtatcgaactgaaaggtatcgatttcaaagaagacggcaatattctgggtcataaactggaatataacttcaattcccacaacgtgtacatcaccgcggataaacagaaaaacggcattaaagccaatttcaaaatccgccataatgtggaagatggtagcgttcagctggccgaccactatcagcaaaacacgccgattggtgatggcccggtcctgctgccggacaatcactacctgagtacccagtccgtgctgtcaaaagatccgaacgaaaaacgtgaccacatggtcctgctggaatttgtgacggctgcgggtatcacccacggcatggacgaactgtataaaaggccttaa BBa_K1994044_sequence 1 cctaggccaggcatcaattgaaatcatcaaactcattatggatttaat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z