Multiple dCas9 binding sites with weak omega promoter upstream of GFP + dCas9 expression cassette
dCas9 with Pam staggered sequence and omega promoter
Part containing only dCas9 enzyme with no terminator or promoter. Interacts with sgRNA to bind to specific DNA target.
RBS used for dCas9 enzyme in team Warwick 2016's project.
Synthetic vector pET-T7p(-12T)-SpacerA-GFP-LVA
Superfolder GFP. 488nm Excitation, 530nm Emission
RBS used for superfolder GFP in team Warwick 2016's project.
Promoter sequence containing multiple PAMs staggered at regular intervals to provide multiple possible sites for dCas9 binding.
sgRNA with PAM-Proximal complementary sequence 5
Conversion of the iGEM parts registry to SBOL2.1