BBa_K1994022
type: ComponentDefinition
persistent identity: BBa K1994022
display id: BBa_K1994022
version: 1
was generated by: iGEM to SBOL conversion
description: Multiple dCas9 binding sites with weak omega promoter upstream of GFP + dCas9 expression cassette
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T08:02:59Z
mutable provenance: dCas9 with Pam staggered sequence and omega promoter
owned by: james
owned by: myers
mutable description: dCas9 with Pam staggered sequence and omega promoter
discontinued: false
dominant: false
experience: experience/None
group u list: _2461_
m user id: 30870
owner id: 28134
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: dCas9 with Pam staggered sequence and omega promoter
star: false
creator: Isobel Holden
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1994019
component: BBa_K1994004
component: BBa_K1994034
component: BBa_K1994018
component: BBa_K1994003
component: BBa_K1994002
component: BBa_K1994044
component: BBa_K1994000
sequence annotation: BBa_K1994019
sequence annotation: BBa_K1994034
sequence annotation: BBa_K1994004
sequence annotation: BBa_K1994003
sequence annotation: BBa_K1994018
sequence annotation: BBa_K1994044
sequence annotation: BBa_K1994002
sequence annotation: BBa_K1994000
sequence: https://synbiohub.org/public/igem/BBa_K1994022_sequence/1

BBa_K1994019
type: Component
persistent identity: component2522849
display id: component2522849
version: 1
top level: BBa_K1994022
definition: BBa_K1994019
access: public

BBa_K1994004
type: Component
persistent identity: component2522856
display id: component2522856
version: 1
top level: BBa_K1994022
definition: BBa_K1994004
access: public

BBa_K1994034
type: Component
persistent identity: component2522853
display id: component2522853
version: 1
top level: BBa_K1994022
definition: BBa_K1994034
access: public

BBa_K1994018
type: Component
persistent identity: component2522858
display id: component2522858
version: 1
top level: BBa_K1994022
definition: BBa_K1994018
access: public

BBa_K1994003
type: Component
persistent identity: component2522857
display id: component2522857
version: 1
top level: BBa_K1994022
definition: BBa_K1994003
access: public

BBa_K1994002
type: Component
persistent identity: component2522862
display id: component2522862
version: 1
top level: BBa_K1994022
definition: BBa_K1994002
access: public

BBa_K1994044
type: Component
persistent identity: component2522861
display id: component2522861
version: 1
top level: BBa_K1994022
definition: BBa_K1994044
access: public

BBa_K1994000
type: Component
persistent identity: component2522863
display id: component2522863
version: 1
top level: BBa_K1994022
definition: BBa_K1994000
access: public

BBa_K1994019
type: SequenceAnnotation
persistent identity: annotation2522849
display id: annotation2522849
version: 1
top level: BBa_K1994022
location: https://synbiohub.org/public/igem/BBa_K1994022/annotation2522849/range2522849/1
component: BBa_K1994019

https://synbiohub.org/public/igem/BBa_K1994022/annotation2522849/range2522849/1
type: Range
persistent identity: range2522849
display id: range2522849
version: 1
top level: BBa_K1994022
direction: direction/reverse
start: 1
end: 239
orientation: inline

BBa_K1994034
type: SequenceAnnotation
persistent identity: annotation2522853
display id: annotation2522853
version: 1
top level: BBa_K1994022
location: https://synbiohub.org/public/igem/BBa_K1994022/annotation2522853/range2522853/1
component: BBa_K1994034

https://synbiohub.org/public/igem/BBa_K1994022/annotation2522853/range2522853/1
type: Range
persistent identity: range2522853
display id: range2522853
version: 1
top level: BBa_K1994022
direction: direction/reverse
start: 248
end: 311
orientation: inline

BBa_K1994004
type: SequenceAnnotation
persistent identity: annotation2522856
display id: annotation2522856
version: 1
top level: BBa_K1994022
location: https://synbiohub.org/public/igem/BBa_K1994022/annotation2522856/range2522856/1
component: BBa_K1994004

https://synbiohub.org/public/igem/BBa_K1994022/annotation2522856/range2522856/1
type: Range
persistent identity: range2522856
display id: range2522856
version: 1
top level: BBa_K1994022
direction: direction/reverse
start: 320
end: 366
orientation: inline

BBa_K1994003
type: SequenceAnnotation
persistent identity: annotation2522857
display id: annotation2522857
version: 1
top level: BBa_K1994022
location: https://synbiohub.org/public/igem/BBa_K1994022/annotation2522857/range2522857/1
component: BBa_K1994003

https://synbiohub.org/public/igem/BBa_K1994022/annotation2522857/range2522857/1
type: Range
persistent identity: range2522857
display id: range2522857
version: 1
top level: BBa_K1994022
direction: direction/reverse
start: 373
end: 1095
orientation: inline

BBa_K1994018
type: SequenceAnnotation
persistent identity: annotation2522858
display id: annotation2522858
version: 1
top level: BBa_K1994022
location: https://synbiohub.org/public/igem/BBa_K1994022/annotation2522858/range2522858/1
component: BBa_K1994018

https://synbiohub.org/public/igem/BBa_K1994022/annotation2522858/range2522858/1
type: Range
persistent identity: range2522858
display id: range2522858
version: 1
top level: BBa_K1994022
direction: direction/reverse
start: 1104
end: 1155
orientation: inline

BBa_K1994044
type: SequenceAnnotation
persistent identity: annotation2522861
display id: annotation2522861
version: 1
top level: BBa_K1994022
location: https://synbiohub.org/public/igem/BBa_K1994022/annotation2522861/range2522861/1
component: BBa_K1994044

https://synbiohub.org/public/igem/BBa_K1994022/annotation2522861/range2522861/1
type: Range
persistent identity: range2522861
display id: range2522861
version: 1
top level: BBa_K1994022
direction: direction/reverse
start: 1164
end: 1211
orientation: inline

BBa_K1994002
type: SequenceAnnotation
persistent identity: annotation2522862
display id: annotation2522862
version: 1
top level: BBa_K1994022
location: https://synbiohub.org/public/igem/BBa_K1994022/annotation2522862/range2522862/1
component: BBa_K1994002

https://synbiohub.org/public/igem/BBa_K1994022/annotation2522862/range2522862/1
type: Range
persistent identity: range2522862
display id: range2522862
version: 1
top level: BBa_K1994022
direction: direction/reverse
start: 1220
end: 1249
orientation: inline

BBa_K1994000
type: SequenceAnnotation
persistent identity: annotation2522863
display id: annotation2522863
version: 1
top level: BBa_K1994022
location: https://synbiohub.org/public/igem/BBa_K1994022/annotation2522863/range2522863/1
component: BBa_K1994000

https://synbiohub.org/public/igem/BBa_K1994022/annotation2522863/range2522863/1
type: Range
persistent identity: range2522863
display id: range2522863
version: 1
top level: BBa_K1994022
direction: direction/reverse
start: 1258
end: 5361
orientation: inline

BBa_K1994000
type: ComponentDefinition
persistent identity: BBa K1994000
display id: BBa_K1994000
version: 1
was generated by: iGEM to SBOL conversion
description: dCas9 enzyme
created: 2016-09-21T11:00:00Z
modified: 2016-09-22T04:26:59Z
mutable provenance: Streptococcus Pyrogenes
owned by: james
owned by: myers
mutable description: Part containing only dCas9 enzyme with no terminator or promoter. Interacts with sgRNA to bind to specific DNA target.
discontinued: false
dominant: false
experience: experience/None
group u list: _2461_
m user id: 27126
owner id: 27126
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: No special design considerations.
star: false
creator: Liam Carroll
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K1994000_sequence/1

BBa_K1994002
type: ComponentDefinition
persistent identity: BBa K1994002
display id: BBa_K1994002
version: 1
was generated by: iGEM to SBOL conversion
description: RBS 173k TIRv1
created: 2016-09-21T11:00:00Z
modified: 2016-09-22T04:24:08Z
mutable provenance: RBS 173k TIRv1
owned by: james
owned by: myers
mutable description: RBS used for dCas9 enzyme in team Warwick 2016's project.
discontinued: false
dominant: false
experience: experience/None
group u list: _2461_
m user id: 27126
owner id: 27126
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: No special design considerations.
star: false
creator: Liam Carroll
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_K1994002_sequence/1

BBa_K1994003
type: ComponentDefinition
persistent identity: BBa K1994003
display id: BBa_K1994003
version: 1
was generated by: iGEM to SBOL conversion
description: Superfolder GFP
created: 2016-09-21T11:00:00Z
modified: 2016-09-22T03:14:22Z
mutable provenance: Synthetic vector pET-T7p(-12T)-SpacerA-GFP-LVA
owned by: james
owned by: myers
mutable description: Superfolder GFP. 488nm Excitation, 530nm Emission
discontinued: false
dominant: false
group u list: _2461_
m user id: 27126
owner id: 27126
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: No special design considerations.
star: false
creator: Liam Carroll
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1994003_sequence/1

BBa_K1994004
type: ComponentDefinition
persistent identity: BBa K1994004
display id: BBa_K1994004
version: 1
was generated by: iGEM to SBOL conversion
description: RBS 28.4k_NEQ RBSCv1.1
created: 2016-09-21T11:00:00Z
modified: 2016-10-19T12:05:49Z
mutable provenance: RBS 28.4k_NEQ RBSCv1.1
owned by: james
owned by: myers
mutable description: RBS used for superfolder GFP in team Warwick 2016's project.
discontinued: false
dominant: false
experience: experience/None
group u list: _2461_
m user id: 30870
owner id: 27126
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: No special design considerations.
star: false
creator: Liam Carroll
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: TGG (Shine-Dalgarno sequence)
sequence annotation: RBS 28.4k_NEQ RBSCv1.1
sequence: https://synbiohub.org/public/igem/BBa_K1994004_sequence/1

TGG (Shine-Dalgarno sequence)
type: SequenceAnnotation
persistent identity: annotation2521745
display id: annotation2521745
version: 1
top level: BBa_K1994004
location: https://synbiohub.org/public/igem/BBa_K1994004/annotation2521745/range2521745/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1994004/annotation2521745/range2521745/1
type: Range
persistent identity: range2521745
display id: range2521745
version: 1
top level: BBa_K1994004
direction: direction/forward
start: 2
end: 11
orientation: inline

RBS 28.4k_NEQ RBSCv1.1
type: SequenceAnnotation
persistent identity: annotation2521776
display id: annotation2521776
version: 1
top level: BBa_K1994004
location: https://synbiohub.org/public/igem/BBa_K1994004/annotation2521776/range2521776/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1994004/annotation2521776/range2521776/1
type: Range
persistent identity: range2521776
display id: range2521776
version: 1
top level: BBa_K1994004
direction: direction/forward
start: 18
end: 47
orientation: inline

BBa_K1994018
type: ComponentDefinition
persistent identity: BBa K1994018
display id: BBa_K1994018
version: 1
was generated by: iGEM to SBOL conversion
description: E. coli PheA terminator
created: 2016-09-25T11:00:00Z
modified: 2016-09-26T08:18:57Z
mutable provenance: Escherichia coli
owned by: james
owned by: myers
mutable description: PheA terminator from E. coli
discontinued: false
dominant: false
group u list: _2461_
m user id: 27126
owner id: 27126
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: No special considerations
star: false
creator: Liam Carroll
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence: https://synbiohub.org/public/igem/BBa_K1994018_sequence/1

BBa_K1994019
type: ComponentDefinition
persistent identity: BBa K1994019
display id: BBa_K1994019
version: 1
was generated by: iGEM to SBOL conversion
description: Multiple dCas9 binding site sequence
created: 2016-09-25T11:00:00Z
modified: 2016-10-19T03:50:49Z
mutable provenance: Escherichia coli
owned by: james
owned by: myers
mutable description: Promoter sequence containing multiple PAMs staggered at regular intervals to provide multiple possible sites for dCas9 binding.
discontinued: false
dominant: false
experience: experience/None
group u list: _2461_
m user id: 30870
owner id: 27126
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: No special considerations
star: false
creator: Liam Carroll
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Multiple dCas9 binding sites
sequence annotation: 30nt dCas9 spacer
sequence: https://synbiohub.org/public/igem/BBa_K1994019_sequence/1

Multiple dCas9 binding sites
type: SequenceAnnotation
persistent identity: annotation2522793
display id: annotation2522793
version: 1
top level: BBa_K1994019
location: https://synbiohub.org/public/igem/BBa_K1994019/annotation2522793/range2522793/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1994019/annotation2522793/range2522793/1
type: Range
persistent identity: range2522793
display id: range2522793
version: 1
top level: BBa_K1994019
direction: direction/forward
start: 7
end: 210
orientation: inline

30nt dCas9 spacer
type: SequenceAnnotation
persistent identity: annotation2522794
display id: annotation2522794
version: 1
top level: BBa_K1994019
location: https://synbiohub.org/public/igem/BBa_K1994019/annotation2522794/range2522794/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1994019/annotation2522794/range2522794/1
type: Range
persistent identity: range2522794
display id: range2522794
version: 1
top level: BBa_K1994019
direction: direction/forward
start: 210
end: 239
orientation: inline

BBa_K1994034
type: ComponentDefinition
persistent identity: BBa K1994034
display id: BBa_K1994034
version: 1
was generated by: iGEM to SBOL conversion
description: Weakened Omega promoter
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T08:11:31Z
mutable provenance: Weakened omega promoter
owned by: james
owned by: myers
mutable description: Weakened omega promoter
discontinued: false
dominant: false
experience: experience/None
group u list: _2461_
m user id: 30870
owner id: 28134
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Weakened omega promoter
star: false
creator: Isobel Holden
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Bgl II
sequence annotation: Weak Sigma 54 promoter
sequence annotation: Sal I
sequence: https://synbiohub.org/public/igem/BBa_K1994034_sequence/1

Bgl II
type: SequenceAnnotation
persistent identity: annotation2522792
display id: annotation2522792
version: 1
top level: BBa_K1994034
location: https://synbiohub.org/public/igem/BBa_K1994034/annotation2522792/range2522792/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1994034/annotation2522792/range2522792/1
type: Range
persistent identity: range2522792
display id: range2522792
version: 1
top level: BBa_K1994034
direction: direction/forward
start: 1
end: 7
orientation: inline

Weak Sigma 54 promoter
type: SequenceAnnotation
persistent identity: annotation2522833
display id: annotation2522833
version: 1
top level: BBa_K1994034
location: https://synbiohub.org/public/igem/BBa_K1994034/annotation2522833/range2522833/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1994034/annotation2522833/range2522833/1
type: Range
persistent identity: range2522833
display id: range2522833
version: 1
top level: BBa_K1994034
direction: direction/forward
start: 8
end: 57
orientation: inline

Sal I
type: SequenceAnnotation
persistent identity: annotation2522809
display id: annotation2522809
version: 1
top level: BBa_K1994034
location: https://synbiohub.org/public/igem/BBa_K1994034/annotation2522809/range2522809/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1994034/annotation2522809/range2522809/1
type: Range
persistent identity: range2522809
display id: range2522809
version: 1
top level: BBa_K1994034
direction: direction/forward
start: 58
end: 64
orientation: inline

BBa_K1994044
type: ComponentDefinition
persistent identity: BBa K1994044
display id: BBa_K1994044
version: 1
was generated by: iGEM to SBOL conversion
description: dCas9 Promoter
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T02:12:18Z
mutable provenance: sgRNA with PAM-Proximal complementary sequence 5
owned by: james
owned by: myers
mutable description: sgRNA with PAM-Proximal complementary sequence 5
discontinued: false
dominant: false
experience: experience/None
group u list: _2461_
m user id: 30870
owner id: 28134
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: sgRNA with PAM-Proximal complementary sequence 5
star: false
creator: Egheosa Ogbomo
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Spacer
sequence annotation: dCas9 Promoter
sequence: https://synbiohub.org/public/igem/BBa_K1994044_sequence/1

Spacer
type: SequenceAnnotation
persistent identity: annotation2522272
display id: annotation2522272
version: 1
top level: BBa_K1994044
location: https://synbiohub.org/public/igem/BBa_K1994044/annotation2522272/range2522272/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1994044/annotation2522272/range2522272/1
type: Range
persistent identity: range2522272
display id: range2522272
version: 1
top level: BBa_K1994044
direction: direction/forward
start: 1
end: 17
orientation: inline

dCas9 Promoter
type: SequenceAnnotation
persistent identity: annotation2522296
display id: annotation2522296
version: 1
top level: BBa_K1994044
location: https://synbiohub.org/public/igem/BBa_K1994044/annotation2522296/range2522296/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K1994044/annotation2522296/range2522296/1
type: Range
persistent identity: range2522296
display id: range2522296
version: 1
top level: BBa_K1994044
direction: direction/forward
start: 18
end: 48
orientation: inline

https://synbiohub.org/public/igem/BBa_K1994022_sequence/1
type: Sequence
persistent identity: BBa K1994022 sequence
display id: BBa_K1994022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagtgggacgaaataggtatgaaacggagtgaagtggagagcgaaggataaacacggaaatagaaggtgaggtaaggatgaatgaggcaaattagggacatatgcggtaagtgtaggtgaaagtgggatgataaaggttgagtacggcgagattaggatatttagggcaagtataggtaaacgaaggctaagaatggaatgagagaggatgattatcgttgttgctagccggcgtggatactagagagatctattcaccgcagggataatcaacactggcacaattcgctgcttgtagctggcagtcgactactagagcttggtattatgctagctactagacaattaatagggaggttctcgagtactagatgcgtaaaggcgaagaactgtttaccggtgtggttccgattctggtggaactggacggcgatgttaatggtcataaattcagtgttcgcggcgaaggtgaaggcgatgcgacgaacggcaaactgaccctgaaatttatctgcaccacgggtaaactgccggtcccgtggccgacgctggtgaccacgctgacctatggcgttcaatgttttgcgcgttacccggatcacatgaaacagcacgactttttcaaatcggccatgccggaaggctatgtgcaggaacgtacgattagctttaaagacgatggtacgtataaaacccgcgcggaagtgaaattcgaaggcgataccctggttaaccgtatcgaactgaaaggtatcgatttcaaagaagacggcaatattctgggtcataaactggaatataacttcaattcccacaacgtgtacatcaccgcggataaacagaaaaacggcattaaagccaatttcaaaatccgccataatgtggaagatggtagcgttcagctggccgaccactatcagcaaaacacgccgattggtgatggcccggtcctgctgccggacaatcactacctgagtacccagtccgtgctgtcaaaagatccgaacgaaaaacgtgaccacatggtcctgctggaatttgtgacggctgcgggtatcacccacggcatggacgaactgtataaaaggccttaatactagaggacgaacaataaggcctcccaaatcggggggccttttttattgataacaaaatactagagcctaggccaggcatcaattgaaatcatcaaactcattatggatttaattactagagcaagggacatcaggaggagggaattatagatactagaggataagaaatactcaataggcttagctatcggcacaaatagcgtcggatgggcggtgatcactgatgaatataaggttccgtctaaaaagttcaaggttctgggaaatacagaccgccacagtatcaaaaaaaatcttataggggctcttttatttgacagtggagagacagcggaagcgactcgtctcaaacggacagctcgtagaaggtatacacgtcggaagaatcgtatttgttatctacaggagattttttcaaatgagatggcgaaagtagatgatagtttctttcatcgacttgaagagtcttttttggtggaagaagacaagaagcatgaacgtcatcctatttttggaaatatagtagatgaagttgcttatcatgagaaatatccaactatctatcatctgcgaaaaaaattggtagattctactgataaagcggatttgcgcttaatctatttggccttagcgcatatgattaagtttcgtggtcattttttgattgagggagatttaaatcctgataatagtgatgtggacaaactatttatccagttggtacaaacctacaatcaattatttgaagaaaaccctattaacgcaagtggagtagatgctaaagcgattctttctgcacgattgagtaaatcaagacgattagaaaatctcattgctcagctccccggtgagaagaaaaatggcttatttgggaatctcattgctttgtcattgggtttgac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1994000_sequence/1
type: Sequence
persistent identity: BBa K1994000 sequence
display id: BBa_K1994000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gataagaaatactcaataggcttagctatcggcacaaatagcgtcggatgggcggtgatcactgatgaatataaggttccgtctaaaaagttcaaggttctgggaaatacagaccgccacagtatcaaaaaaaatcttataggggctcttttatttgacagtggagagacagcggaagcgactcgtctcaaacggacagctcgtagaaggtatacacgtcggaagaatcgtatttgttatctacaggagattttttcaaatgagatggcgaaagtagatgatagtttctttcatcgacttgaagagtcttttttggtggaagaagacaagaagcatgaacgtcatcctatttttggaaatatagtagatgaagttgcttatcatgagaaatatccaactatctatcatctgcgaaaaaaattggtagattctactgataaagcggatttgcgcttaatctatttggccttagcgcatatgattaagtttcgtggtcattttttgattgagggagatttaaatcctgataatagtgatgtggacaaactatttatccagttggtacaaacctacaatcaattatttgaagaaaaccctattaacgcaagtggagtagatgctaaagcgattctttctgcacgattgagtaaatcaagacgattagaaaatctcattgctcagctccccggtgagaagaaaaatggcttatttgggaatctcattgctttgtcattgggtttgacccctaattttaaatcaaattttgatttggcagaagatgctaaattacagctttcaaaagatacttacgatgatgatttagataatttattggcgcaaattggagatcaatatgctgatttgtttttggcagctaagaatttatcagatgctattttactttcagatatcctaagagtaaatactgaaataactaaggctcccctatcagcttcaatgattaaacgctacgatgaacatcatcaagacttgactcttttaaaagctttagttcgacaacaacttccagaaaagtataaagaaatcttttttgatcaatcaaaaaacggatatgcaggttatattgatgggggagctagccaagaagaattttataaatttatcaaaccaattttagaaaaaatggatggtactgaggaattattggtgaaactaaatcgtgaagatttgctgcgcaagcaacggacctttgacaacggctctattccccatcaaattcacttgggtgagctgcatgctattttgagaagacaagaagacttttatccatttttaaaagacaatcgtgagaagattgaaaaaatcttgacttttcgtattccttattatgttggtccattggcgcgtggcaatagtcgttttgcatggatgactcggaagtctgaagaaacaattaccccatggaattttgaagaagttgtcgataaaggtgcttcagctcaatcatttattgaacgcatgacaaactttgataaaaatcttccaaatgaaaaagtactaccaaaacatagtttgctttatgagtattttacggtttataacgaattgacaaaggtcaaatatgttactgaaggaatgcgaaaaccagcatttctttcaggtgaacagaagaaagccattgttgatttactcttcaaaacaaatcgaaaagtaaccgttaagcaattaaaagaagattatttcaaaaaaatagaatgttttgatagtgttgaaatttcaggagttgaagatagatttaatgcttcattaggtacctaccatgatttgctaaaaattattaaagataaagattttttggataatgaagaaaatgaagatatcttagaggatattgttttaacattgaccttatttgaagatagggagatgattgaggaaagacttaaaacatatgctcacctctttgatgataaggtgatgaaacagcttaaacgtcgccgttatactggttggggacgtttgtctcgaaaattgattaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1994002_sequence/1
type: Sequence
persistent identity: BBa K1994002 sequence
display id: BBa_K1994002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caagggacatcaggaggagggaattataga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1994003_sequence/1
type: Sequence
persistent identity: BBa K1994003 sequence
display id: BBa_K1994003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggcgaagaactgtttaccggtgtggttccgattctggtggaactggacggcgatgttaatggtcataaattcagtgttcgcggcgaaggtgaaggcgatgcgacgaacggcaaactgaccctgaaatttatctgcaccacgggtaaactgccggtcccgtggccgacgctggtgaccacgctgacctatggcgttcaatgttttgcgcgttacccggatcacatgaaacagcacgactttttcaaatcggccatgccggaaggctatgtgcaggaacgtacgattagctttaaagacgatggtacgtataaaacccgcgcggaagtgaaattcgaaggcgataccctggttaaccgtatcgaactgaaaggtatcgatttcaaagaagacggcaatattctgggtcataaactggaatataacttcaattcccacaacgtgtacatcaccgcggataaacagaaaaacggcattaaagccaatttcaaaatccgccataatgtggaagatggtagcgttcagctggccgaccactatcagcaaaacacgccgattggtgatggcccggtcctgctgccggacaatcactacctgagtacccagtccgtgctgtcaaaagatccgaacgaaaaacgtgaccacatggtcctgctggaatttgtgacggctgcgggtatcacccacggcatggacgaactgtataaaaggccttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1994004_sequence/1
type: Sequence
persistent identity: BBa K1994004 sequence
display id: BBa_K1994004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cttggtattatgctagctactagacaattaatagggaggttctcgag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1994018_sequence/1
type: Sequence
persistent identity: BBa K1994018 sequence
display id: BBa_K1994018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gacgaacaataaggcctcccaaatcggggggccttttttattgataacaaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1994019_sequence/1
type: Sequence
persistent identity: BBa K1994019 sequence
display id: BBa_K1994019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagtgggacgaaataggtatgaaacggagtgaagtggagagcgaaggataaacacggaaatagaaggtgaggtaaggatgaatgaggcaaattagggacatatgcggtaagtgtaggtgaaagtgggatgataaaggttgagtacggcgagattaggatatttagggcaagtataggtaaacgaaggctaagaatggaatgagagaggatgattatcgttgttgctagccggcgtgga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1994034_sequence/1
type: Sequence
persistent identity: BBa K1994034 sequence
display id: BBa_K1994034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agatctattcaccgcagggataatcaacactggcacaattcgctgcttgtagctggcagtcgac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1994044_sequence/1
type: Sequence
persistent identity: BBa K1994044 sequence
display id: BBa_K1994044_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctaggccaggcatcaattgaaatcatcaaactcattatggatttaat
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z