LOCUS BBa_K1994025 5075 bp DNA linear 02-Jan-2026 DEFINITION BsaI-GFP-dCas9 ACCESSION BBa_K1994025 VERSION BBa_K1994025.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=BBa_K1994005 misc_feature 30..39 /label=TGG (Shine-Dalgarno sequence) RBS 46..75 /label=RBS 28.4k_NEQ RBSCv1.1 RBS 29..75 /label=BBa_K1994004 misc_feature 82..804 /label=BBa_K1994003 misc_feature 813..815 /label=spacer stem_loop 816..864 /label=T7 Terminator misc_feature 865..869 /label=Spacer terminator 813..869 /label=BBa_K1994045 misc_feature 878..894 /label=Spacer promoter 895..925 /label=dCas9 Promoter promoter 878..925 /label=BBa_K1994044 RBS 934..963 /label=BBa_K1994002 CDS 972..5075 /label=BBa_K1994000 ORIGIN 1 agagaccact ttcggtctct tactagagct tggtattatg ctagctacta gacaattaat 61 agggaggttc tcgagtacta gatgcgtaaa ggcgaagaac tgtttaccgg tgtggttccg 121 attctggtgg aactggacgg cgatgttaat ggtcataaat tcagtgttcg cggcgaaggt 181 gaaggcgatg cgacgaacgg caaactgacc ctgaaattta tctgcaccac gggtaaactg 241 ccggtcccgt ggccgacgct ggtgaccacg ctgacctatg gcgttcaatg ttttgcgcgt 301 tacccggatc acatgaaaca gcacgacttt ttcaaatcgg ccatgccgga aggctatgtg 361 caggaacgta cgattagctt taaagacgat ggtacgtata aaacccgcgc ggaagtgaaa 421 ttcgaaggcg ataccctggt taaccgtatc gaactgaaag gtatcgattt caaagaagac 481 ggcaatattc tgggtcataa actggaatat aacttcaatt cccacaacgt gtacatcacc 541 gcggataaac agaaaaacgg cattaaagcc aatttcaaaa tccgccataa tgtggaagat 601 ggtagcgttc agctggccga ccactatcag caaaacacgc cgattggtga tggcccggtc 661 ctgctgccgg acaatcacta cctgagtacc cagtccgtgc tgtcaaaaga tccgaacgaa 721 aaacgtgacc acatggtcct gctggaattt gtgacggctg cgggtatcac ccacggcatg 781 gacgaactgt ataaaaggcc ttaatactag agtaatactc gaacccctag cccgctctta 841 tcgggcggct aggggttttt tgtcctagat actagagcct aggccaggca tcaattgaaa 901 tcatcaaact cattatggat ttaattacta gagcaaggga catcaggagg agggaattat 961 agatactaga ggataagaaa tactcaatag gcttagctat cggcacaaat agcgtcggat 1021 gggcggtgat cactgatgaa tataaggttc cgtctaaaaa gttcaaggtt ctgggaaata 1081 cagaccgcca cagtatcaaa aaaaatctta taggggctct tttatttgac agtggagaga 1141 cagcggaagc gactcgtctc aaacggacag ctcgtagaag gtatacacgt cggaagaatc 1201 gtatttgtta tctacaggag attttttcaa atgagatggc gaaagtagat gatagtttct 1261 ttcatcgact tgaagagtct tttttggtgg aagaagacaa gaagcatgaa cgtcatccta 1321 tttttggaaa tatagtagat gaagttgctt atcatgagaa atatccaact atctatcatc 1381 tgcgaaaaaa attggtagat tctactgata aagcggattt gcgcttaatc tatttggcct 1441 tagcgcatat gattaagttt cgtggtcatt ttttgattga gggagattta aatcctgata 1501 atagtgatgt ggacaaacta tttatccagt tggtacaaac ctacaatcaa ttatttgaag 1561 aaaaccctat taacgcaagt ggagtagatg ctaaagcgat tctttctgca cgattgagta 1621 aatcaagacg attagaaaat ctcattgctc agctccccgg tgagaagaaa aatggcttat 1681 ttgggaatct cattgctttg tcattgggtt tgacccctaa ttttaaatca aattttgatt 1741 tggcagaaga tgctaaatta cagctttcaa aagatactta cgatgatgat ttagataatt 1801 tattggcgca aattggagat caatatgctg atttgttttt ggcagctaag aatttatcag 1861 atgctatttt actttcagat atcctaagag taaatactga aataactaag gctcccctat 1921 cagcttcaat gattaaacgc tacgatgaac atcatcaaga cttgactctt ttaaaagctt 1981 tagttcgaca acaacttcca gaaaagtata aagaaatctt ttttgatcaa tcaaaaaacg 2041 gatatgcagg ttatattgat gggggagcta gccaagaaga attttataaa tttatcaaac 2101 caattttaga aaaaatggat ggtactgagg aattattggt gaaactaaat cgtgaagatt 2161 tgctgcgcaa gcaacggacc tttgacaacg gctctattcc ccatcaaatt cacttgggtg 2221 agctgcatgc tattttgaga agacaagaag acttttatcc atttttaaaa gacaatcgtg 2281 agaagattga aaaaatcttg acttttcgta ttccttatta tgttggtcca ttggcgcgtg 2341 gcaatagtcg ttttgcatgg atgactcgga agtctgaaga aacaattacc ccatggaatt 2401 ttgaagaagt tgtcgataaa ggtgcttcag ctcaatcatt tattgaacgc atgacaaact 2461 ttgataaaaa tcttccaaat gaaaaagtac taccaaaaca tagtttgctt tatgagtatt 2521 ttacggttta taacgaattg acaaaggtca aatatgttac tgaaggaatg cgaaaaccag 2581 catttctttc aggtgaacag aagaaagcca ttgttgattt actcttcaaa acaaatcgaa 2641 aagtaaccgt taagcaatta aaagaagatt atttcaaaaa aatagaatgt tttgatagtg 2701 ttgaaatttc aggagttgaa gatagattta atgcttcatt aggtacctac catgatttgc 2761 taaaaattat taaagataaa gattttttgg ataatgaaga aaatgaagat atcttagagg 2821 atattgtttt aacattgacc ttatttgaag atagggagat gattgaggaa agacttaaaa 2881 catatgctca cctctttgat gataaggtga tgaaacagct taaacgtcgc cgttatactg 2941 gttggggacg tttgtctcga aaattgatta atggtattag ggataagcaa tctggcaaaa 3001 caatattaga ttttttgaaa tcagatggtt ttgccaatcg caattttatg cagctgatcc 3061 atgatgatag tttgacattt aaagaagaca ttcaaaaagc acaagtgtct ggacaaggcg 3121 atagtttaca tgaacatatt gcaaatttag ctggtagccc tgctattaaa aaaggtattt 3181 tacagactgt aaaagttgtt gatgaattgg tcaaagtaat ggggcggcat aagccagaaa 3241 atatcgttat tgaaatggca cgtgaaaatc agacaactca aaagggccag aaaaattcgc 3301 gagagcgtat gaaacgaatc gaagaaggta tcaaagaatt aggaagtcag attcttaaag 3361 agcatcctgt tgaaaatact caattgcaaa atgaaaagct ctatctctat tatctccaaa 3421 atggaagaga catgtatgtg gaccaagaat tagatattaa tcgtttaagt gattatgatg 3481 tcgatgccat tgttccacaa agtttcctta aagacgattc aatagacaat aaggtcttaa 3541 cgcgttctga taaaaatcgt ggtaaatcgg ataacgttcc aagtgaagaa gtagtcaaaa 3601 agatgaaaaa ctattggaga caacttctaa acgccaagtt aatcactcaa cgtaagtttg 3661 ataatttaac gaaagctgaa cgtggaggtt tgagtgaact tgataaagct ggttttatca 3721 aacgccaatt ggttgaaact cgccaaatca ctaagcatgt ggcacaaatt ttggatagtc 3781 gcatgaatac taaatacgat gaaaatgata aacttattcg agaggttaaa gtgattacct 3841 taaaatctaa attagtttct gacttccgaa aagatttcca attctataaa gtacgtgaga 3901 ttaacaatta ccatcatgcc catgatgcgt atctaaatgc cgtcgttgga actgctttga 3961 ttaagaaata tccaaaactt gaatcggagt ttgtctatgg tgattataaa gtttatgatg 4021 ttcgtaaaat gattgctaag tctgagcaag aaataggcaa agcaaccgca aaatatttct 4081 tttactctaa tatcatgaac ttcttcaaaa cagaaattac acttgcaaat ggagagattc 4141 gcaaacgccc tctaatcgaa actaatgggg aaactggaga aattgtctgg gataaagggc 4201 gagattttgc cacagtgcgc aaagtattgt ccatgcccca agtcaatatt gtcaagaaaa 4261 cagaagtaca gacaggcgga ttctccaagg agtcaatttt accaaaaaga aattcggaca 4321 agcttattgc tcgtaaaaaa gactgggatc caaaaaaata tggtggtttt gatagtccaa 4381 cggtagctta ttcagtccta gtggttgcta aggtggaaaa agggaaatcg aagaagttaa 4441 aatccgttaa agagttacta gggatcacaa ttatggaaag aagttccttt gaaaaaaatc 4501 cgattgactt tttagaagct aaaggatata aggaagttaa aaaagactta atcattaaac 4561 tacctaaata tagtcttttt gagttagaaa acggtcgtaa acggatgctg gctagtgccg 4621 gagaattaca aaaaggaaat gagctggctc tgccaagcaa atatgtgaat tttttatatt 4681 tagctagtca ttatgaaaag ttgaagggta gtccagaaga taacgaacaa aaacaattgt 4741 ttgtggagca gcataagcat tatttagatg agattattga gcaaatcagt gaattttcta 4801 agcgtgttat tttagcagat gccaatttag ataaagttct tagtgcatat aacaaacata 4861 gagacaaacc aatacgtgaa caagcagaaa atattattca tttatttacg ttgacgaatc 4921 ttggagctcc cgctgctttt aaatattttg atacaacaat tgatcgtaaa cgatatacgt 4981 ctacaaaaga agttttagat gccactctta tccatcaatc catcactggt ctttatgaaa 5041 cacgcattga tttgagtcag ctaggaggtg actga //