##gff-version 3
##sequence-region BBa_K1994025 1 5075
BBa_K1994025	.	engineered_region	82	804	.	+	0	ID=BBa_K1994003;Name=BBa_K1994003
BBa_K1994025	.	ribosome_entry_site	29	75	.	+	0	ID=BBa_K1994004;Name=BBa_K1994004
BBa_K1994025	.	ribosome_entry_site	46	75	.	+	0	ID=annotation2521776;Name=RBS 28.4k_NEQ RBSCv1.1;Parent=BBa_K1994004
BBa_K1994025	.	sequence_feature	30	39	.	+	0	ID=annotation2521745;Name=TGG (Shine-Dalgarno sequence);Parent=BBa_K1994004
BBa_K1994025	.	promoter	878	925	.	+	0	ID=BBa_K1994044;Name=BBa_K1994044
BBa_K1994025	.	promoter	895	925	.	+	0	ID=annotation2522296;Name=dCas9 Promoter;Parent=BBa_K1994044
BBa_K1994025	.	sequence_feature	878	894	.	+	0	ID=annotation2522272;Name=Spacer;Parent=BBa_K1994044
BBa_K1994025	.	terminator	813	869	.	+	0	ID=BBa_K1994045;Name=BBa_K1994045
BBa_K1994025	.	sequence_feature	865	869	.	+	0	ID=annotation2521969;Name=Spacer;Parent=BBa_K1994045
BBa_K1994025	.	sequence_feature	813	815	.	+	0	ID=annotation2521935;Name=spacer;Parent=BBa_K1994045
BBa_K1994025	.	stem_loop	816	864	.	+	0	ID=annotation2521968;Name=T7 Terminator;Parent=BBa_K1994045
BBa_K1994025	.	sequence_feature	1	20	.	+	0	ID=BBa_K1994005;Name=BBa_K1994005
BBa_K1994025	.	CDS	972	5075	.	+	0	ID=BBa_K1994000;Name=BBa_K1994000
BBa_K1994025	.	ribosome_entry_site	934	963	.	+	0	ID=BBa_K1994002;Name=BBa_K1994002
>BBa_K1994025
agagaccactttcggtctcttactagagcttggtattatgctagctactagacaattaatagggaggttctcgagtact
agatgcgtaaaggcgaagaactgtttaccggtgtggttccgattctggtggaactggacggcgatgttaatggtcataa
attcagtgttcgcggcgaaggtgaaggcgatgcgacgaacggcaaactgaccctgaaatttatctgcaccacgggtaaa
ctgccggtcccgtggccgacgctggtgaccacgctgacctatggcgttcaatgttttgcgcgttacccggatcacatga
aacagcacgactttttcaaatcggccatgccggaaggctatgtgcaggaacgtacgattagctttaaagacgatggtac
gtataaaacccgcgcggaagtgaaattcgaaggcgataccctggttaaccgtatcgaactgaaaggtatcgatttcaaa
gaagacggcaatattctgggtcataaactggaatataacttcaattcccacaacgtgtacatcaccgcggataaacaga
aaaacggcattaaagccaatttcaaaatccgccataatgtggaagatggtagcgttcagctggccgaccactatcagca
aaacacgccgattggtgatggcccggtcctgctgccggacaatcactacctgagtacccagtccgtgctgtcaaaagat
ccgaacgaaaaacgtgaccacatggtcctgctggaatttgtgacggctgcgggtatcacccacggcatggacgaactgt
ataaaaggccttaatactagagtaatactcgaacccctagcccgctcttatcgggcggctaggggttttttgtcctaga
tactagagcctaggccaggcatcaattgaaatcatcaaactcattatggatttaattactagagcaagggacatcagga
ggagggaattatagatactagaggataagaaatactcaataggcttagctatcggcacaaatagcgtcggatgggcggt
gatcactgatgaatataaggttccgtctaaaaagttcaaggttctgggaaatacagaccgccacagtatcaaaaaaaat
cttataggggctcttttatttgacagtggagagacagcggaagcgactcgtctcaaacggacagctcgtagaaggtata
cacgtcggaagaatcgtatttgttatctacaggagattttttcaaatgagatggcgaaagtagatgatagtttctttca
tcgacttgaagagtcttttttggtggaagaagacaagaagcatgaacgtcatcctatttttggaaatatagtagatgaa
gttgcttatcatgagaaatatccaactatctatcatctgcgaaaaaaattggtagattctactgataaagcggatttgc
gcttaatctatttggccttagcgcatatgattaagtttcgtggtcattttttgattgagggagatttaaatcctgataa
tagtgatgtggacaaactatttatccagttggtacaaacctacaatcaattatttgaagaaaaccctattaacgcaagt
ggagtagatgctaaagcgattctttctgcacgattgagtaaatcaagacgattagaaaatctcattgctcagctccccg
gtgagaagaaaaatggcttatttgggaatctcattgctttgtcattgggtttgacccctaattttaaatcaaattttga
tttggcagaagatgctaaattacagctttcaaaagatacttacgatgatgatttagataatttattggcgcaaattgga
gatcaatatgctgatttgtttttggcagctaagaatttatcagatgctattttactttcagatatcctaagagtaaata
ctgaaataactaaggctcccctatcagcttcaatgattaaacgctacgatgaacatcatcaagacttgactcttttaaa
agctttagttcgacaacaacttccagaaaagtataaagaaatcttttttgatcaatcaaaaaacggatatgcaggttat
attgatgggggagctagccaagaagaattttataaatttatcaaaccaattttagaaaaaatggatggtactgaggaat
tattggtgaaactaaatcgtgaagatttgctgcgcaagcaacggacctttgacaacggctctattccccatcaaattca
cttgggtgagctgcatgctattttgagaagacaagaagacttttatccatttttaaaagacaatcgtgagaagattgaa
aaaatcttgacttttcgtattccttattatgttggtccattggcgcgtggcaatagtcgttttgcatggatgactcgga
agtctgaagaaacaattaccccatggaattttgaagaagttgtcgataaaggtgcttcagctcaatcatttattgaacg
catgacaaactttgataaaaatcttccaaatgaaaaagtactaccaaaacatagtttgctttatgagtattttacggtt
tataacgaattgacaaaggtcaaatatgttactgaaggaatgcgaaaaccagcatttctttcaggtgaacagaagaaag
ccattgttgatttactcttcaaaacaaatcgaaaagtaaccgttaagcaattaaaagaagattatttcaaaaaaataga
atgttttgatagtgttgaaatttcaggagttgaagatagatttaatgcttcattaggtacctaccatgatttgctaaaa
attattaaagataaagattttttggataatgaagaaaatgaagatatcttagaggatattgttttaacattgaccttat
ttgaagatagggagatgattgaggaaagacttaaaacatatgctcacctctttgatgataaggtgatgaaacagcttaa
acgtcgccgttatactggttggggacgtttgtctcgaaaattgattaatggtattagggataagcaatctggcaaaaca
atattagattttttgaaatcagatggttttgccaatcgcaattttatgcagctgatccatgatgatagtttgacattta
aagaagacattcaaaaagcacaagtgtctggacaaggcgatagtttacatgaacatattgcaaatttagctggtagccc
tgctattaaaaaaggtattttacagactgtaaaagttgttgatgaattggtcaaagtaatggggcggcataagccagaa
aatatcgttattgaaatggcacgtgaaaatcagacaactcaaaagggccagaaaaattcgcgagagcgtatgaaacgaa
tcgaagaaggtatcaaagaattaggaagtcagattcttaaagagcatcctgttgaaaatactcaattgcaaaatgaaaa
gctctatctctattatctccaaaatggaagagacatgtatgtggaccaagaattagatattaatcgtttaagtgattat
gatgtcgatgccattgttccacaaagtttccttaaagacgattcaatagacaataaggtcttaacgcgttctgataaaa
atcgtggtaaatcggataacgttccaagtgaagaagtagtcaaaaagatgaaaaactattggagacaacttctaaacgc
caagttaatcactcaacgtaagtttgataatttaacgaaagctgaacgtggaggtttgagtgaacttgataaagctggt
tttatcaaacgccaattggttgaaactcgccaaatcactaagcatgtggcacaaattttggatagtcgcatgaatacta
aatacgatgaaaatgataaacttattcgagaggttaaagtgattaccttaaaatctaaattagtttctgacttccgaaa
agatttccaattctataaagtacgtgagattaacaattaccatcatgcccatgatgcgtatctaaatgccgtcgttgga
actgctttgattaagaaatatccaaaacttgaatcggagtttgtctatggtgattataaagtttatgatgttcgtaaaa
tgattgctaagtctgagcaagaaataggcaaagcaaccgcaaaatatttcttttactctaatatcatgaacttcttcaa
aacagaaattacacttgcaaatggagagattcgcaaacgccctctaatcgaaactaatggggaaactggagaaattgtc
tgggataaagggcgagattttgccacagtgcgcaaagtattgtccatgccccaagtcaatattgtcaagaaaacagaag
tacagacaggcggattctccaaggagtcaattttaccaaaaagaaattcggacaagcttattgctcgtaaaaaagactg
ggatccaaaaaaatatggtggttttgatagtccaacggtagcttattcagtcctagtggttgctaaggtggaaaaaggg
aaatcgaagaagttaaaatccgttaaagagttactagggatcacaattatggaaagaagttcctttgaaaaaaatccga
ttgactttttagaagctaaaggatataaggaagttaaaaaagacttaatcattaaactacctaaatatagtctttttga
gttagaaaacggtcgtaaacggatgctggctagtgccggagaattacaaaaaggaaatgagctggctctgccaagcaaa
tatgtgaattttttatatttagctagtcattatgaaaagttgaagggtagtccagaagataacgaacaaaaacaattgt
ttgtggagcagcataagcattatttagatgagattattgagcaaatcagtgaattttctaagcgtgttattttagcaga
tgccaatttagataaagttcttagtgcatataacaaacatagagacaaaccaatacgtgaacaagcagaaaatattatt
catttatttacgttgacgaatcttggagctcccgctgcttttaaatattttgatacaacaattgatcgtaaacgatata
cgtctacaaaagaagttttagatgccactcttatccatcaatccatcactggtctttatgaaacacgcattgatttgag
tcagctaggaggtgactga