LOCUS       BBa_K1994028             177 bp    DNA     linear       23-Dec-2024
DEFINITION  sgRNA with PAM Proximal complementary sequence and Com binding 
            protein handle
ACCESSION   BBa_K1994028
VERSION     BBa_K1994028.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=Golden Gate adaptor
     stem_loop       20..62
                     /label=dCas9 handle
     stem_loop       63..97
                     /label=truncated s.pyrogenes terminator
     misc_feature    98..108
                     /label=10bp spacer
     stem_loop       109..128
                     /label=Com BP handle
     misc_feature    129..139
                     /label=10bp spacer
     stem_loop       140..177
                     /label=aspA terminator
ORIGIN
        1 agagaccact ttcggtctct gttttagagc tagaaatagc aagttaaaat aaggctagtc
       61 cgttatcaac ttgaaaaagt ggcaccgagt cggtgcacca ataccactga atgcctgcga
      121 gcatcaccaa taccaacaag aaaaaaggca cgtcatctga cgtgcctttt ttattta
//