LOCUS       BBa_K1994031             223 bp    DNA     linear       27-Dec-2024
DEFINITION  sgRNA with PAM Proximal complementary sequence and MS2 binding 
            protein handle
ACCESSION   BBa_K1994031
VERSION     BBa_K1994031.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=Golden Gate
     stem_loop       21..63
                     /label=dCas9 handle
     stem_loop       64..98
                     /label=truncated S.pyrogenes terminator 
     misc_feature    99..109
                     /label=10bp linker
     stem_loop       110..135
                     /label=MS2 BP handle
     misc_feature    136..146
                     /label=10bp linker
     stem_loop       147..223
                     /label=aspA terminator
ORIGIN
        1 ctagattgac agctagctca gtcctaggta taatgctagc agagaccact ttcggtctct
       61 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
      121 ggcaccgagt cggtgcaccc aacaaagcgc acatgaggat tacccatgtg cacccaacaa
      181 aacaagaaaa aaggcacgtc atctgacgtg ccttttttat tta
//