LOCUS       BBa_K1994032             293 bp    DNA     linear       28-May-2024
DEFINITION  Multiple dCas9 binding sites with weak Sigma 54 promoter
ACCESSION   BBa_K1994032
VERSION     BBa_K1994032.1  
FEATURES             Location/Qualifiers
     misc_feature    7..210
                     /label=Multiple dCas9 binding sites 
     misc_feature    210..239
                     /label=30nt dCas9 spacer
     promoter        1..239
                     /label=BBa_K1994019
     misc_feature    248..253
                     /label=Bgl II
     misc_feature    254..287
                     /label=Weak Sigma 54
     misc_feature    288..293
                     /label=Sal I
     promoter        248..293
                     /label=BBa_K1994033
ORIGIN
        1 agagtgggac gaaataggta tgaaacggag tgaagtggag agcgaaggat aaacacggaa
       61 atagaaggtg aggtaaggat gaatgaggca aattagggac atatgcggta agtgtaggtg
      121 aaagtgggat gataaaggtt gagtacggcg agattaggat atttagggca agtataggta
      181 aacgaaggct aagaatggaa tgagagagga tgattatcgt tgttgctagc cggcgtggat
      241 actagagaga tctttgacag ctagctcagt cctagggatt gtgctaggtc gac
//