LOCUS BBa_K1994060 5042 bp DNA linear 18-Feb-2025 DEFINITION Multiple dCas9 binding sites with weak sigma 54 promoter upstream of GFP + dCas9 expression cassette ACCESSION BBa_K1994060 VERSION BBa_K1994060.1 FEATURES Location/Qualifiers misc_feature 2..11 /label=TGG (Shine-Dalgarno sequence) RBS 18..47 /label=RBS 28.4k_NEQ RBSCv1.1 RBS 1..47 /label=BBa_K1994004 misc_feature 54..776 /label=BBa_K1994003 terminator 785..836 /label=BBa_K1994018 misc_feature 845..861 /label=Spacer promoter 862..892 /label=dCas9 Promoter promoter 845..892 /label=BBa_K1994044 RBS 901..930 /label=BBa_K1994002 CDS 939..5042 /label=BBa_K1994000 ORIGIN 1 cttggtatta tgctagctac tagacaatta atagggaggt tctcgagtac tagatgcgta 61 aaggcgaaga actgtttacc ggtgtggttc cgattctggt ggaactggac ggcgatgtta 121 atggtcataa attcagtgtt cgcggcgaag gtgaaggcga tgcgacgaac ggcaaactga 181 ccctgaaatt tatctgcacc acgggtaaac tgccggtccc gtggccgacg ctggtgacca 241 cgctgaccta tggcgttcaa tgttttgcgc gttacccgga tcacatgaaa cagcacgact 301 ttttcaaatc ggccatgccg gaaggctatg tgcaggaacg tacgattagc tttaaagacg 361 atggtacgta taaaacccgc gcggaagtga aattcgaagg cgataccctg gttaaccgta 421 tcgaactgaa aggtatcgat ttcaaagaag acggcaatat tctgggtcat aaactggaat 481 ataacttcaa ttcccacaac gtgtacatca ccgcggataa acagaaaaac ggcattaaag 541 ccaatttcaa aatccgccat aatgtggaag atggtagcgt tcagctggcc gaccactatc 601 agcaaaacac gccgattggt gatggcccgg tcctgctgcc ggacaatcac tacctgagta 661 cccagtccgt gctgtcaaaa gatccgaacg aaaaacgtga ccacatggtc ctgctggaat 721 ttgtgacggc tgcgggtatc acccacggca tggacgaact gtataaaagg ccttaatact 781 agaggacgaa caataaggcc tcccaaatcg gggggccttt tttattgata acaaaatact 841 agagcctagg ccaggcatca attgaaatca tcaaactcat tatggattta attactagag 901 caagggacat caggaggagg gaattataga tactagagga taagaaatac tcaataggct 961 tagctatcgg cacaaatagc gtcggatggg cggtgatcac tgatgaatat aaggttccgt 1021 ctaaaaagtt caaggttctg ggaaatacag accgccacag tatcaaaaaa aatcttatag 1081 gggctctttt atttgacagt ggagagacag cggaagcgac tcgtctcaaa cggacagctc 1141 gtagaaggta tacacgtcgg aagaatcgta tttgttatct acaggagatt ttttcaaatg 1201 agatggcgaa agtagatgat agtttctttc atcgacttga agagtctttt ttggtggaag 1261 aagacaagaa gcatgaacgt catcctattt ttggaaatat agtagatgaa gttgcttatc 1321 atgagaaata tccaactatc tatcatctgc gaaaaaaatt ggtagattct actgataaag 1381 cggatttgcg cttaatctat ttggccttag cgcatatgat taagtttcgt ggtcattttt 1441 tgattgaggg agatttaaat cctgataata gtgatgtgga caaactattt atccagttgg 1501 tacaaaccta caatcaatta tttgaagaaa accctattaa cgcaagtgga gtagatgcta 1561 aagcgattct ttctgcacga ttgagtaaat caagacgatt agaaaatctc attgctcagc 1621 tccccggtga gaagaaaaat ggcttatttg ggaatctcat tgctttgtca ttgggtttga 1681 cccctaattt taaatcaaat tttgatttgg cagaagatgc taaattacag ctttcaaaag 1741 atacttacga tgatgattta gataatttat tggcgcaaat tggagatcaa tatgctgatt 1801 tgtttttggc agctaagaat ttatcagatg ctattttact ttcagatatc ctaagagtaa 1861 atactgaaat aactaaggct cccctatcag cttcaatgat taaacgctac gatgaacatc 1921 atcaagactt gactctttta aaagctttag ttcgacaaca acttccagaa aagtataaag 1981 aaatcttttt tgatcaatca aaaaacggat atgcaggtta tattgatggg ggagctagcc 2041 aagaagaatt ttataaattt atcaaaccaa ttttagaaaa aatggatggt actgaggaat 2101 tattggtgaa actaaatcgt gaagatttgc tgcgcaagca acggaccttt gacaacggct 2161 ctattcccca tcaaattcac ttgggtgagc tgcatgctat tttgagaaga caagaagact 2221 tttatccatt tttaaaagac aatcgtgaga agattgaaaa aatcttgact tttcgtattc 2281 cttattatgt tggtccattg gcgcgtggca atagtcgttt tgcatggatg actcggaagt 2341 ctgaagaaac aattacccca tggaattttg aagaagttgt cgataaaggt gcttcagctc 2401 aatcatttat tgaacgcatg acaaactttg ataaaaatct tccaaatgaa aaagtactac 2461 caaaacatag tttgctttat gagtatttta cggtttataa cgaattgaca aaggtcaaat 2521 atgttactga aggaatgcga aaaccagcat ttctttcagg tgaacagaag aaagccattg 2581 ttgatttact cttcaaaaca aatcgaaaag taaccgttaa gcaattaaaa gaagattatt 2641 tcaaaaaaat agaatgtttt gatagtgttg aaatttcagg agttgaagat agatttaatg 2701 cttcattagg tacctaccat gatttgctaa aaattattaa agataaagat tttttggata 2761 atgaagaaaa tgaagatatc ttagaggata ttgttttaac attgacctta tttgaagata 2821 gggagatgat tgaggaaaga cttaaaacat atgctcacct ctttgatgat aaggtgatga 2881 aacagcttaa acgtcgccgt tatactggtt ggggacgttt gtctcgaaaa ttgattaatg 2941 gtattaggga taagcaatct ggcaaaacaa tattagattt tttgaaatca gatggttttg 3001 ccaatcgcaa ttttatgcag ctgatccatg atgatagttt gacatttaaa gaagacattc 3061 aaaaagcaca agtgtctgga caaggcgata gtttacatga acatattgca aatttagctg 3121 gtagccctgc tattaaaaaa ggtattttac agactgtaaa agttgttgat gaattggtca 3181 aagtaatggg gcggcataag ccagaaaata tcgttattga aatggcacgt gaaaatcaga 3241 caactcaaaa gggccagaaa aattcgcgag agcgtatgaa acgaatcgaa gaaggtatca 3301 aagaattagg aagtcagatt cttaaagagc atcctgttga aaatactcaa ttgcaaaatg 3361 aaaagctcta tctctattat ctccaaaatg gaagagacat gtatgtggac caagaattag 3421 atattaatcg tttaagtgat tatgatgtcg atgccattgt tccacaaagt ttccttaaag 3481 acgattcaat agacaataag gtcttaacgc gttctgataa aaatcgtggt aaatcggata 3541 acgttccaag tgaagaagta gtcaaaaaga tgaaaaacta ttggagacaa cttctaaacg 3601 ccaagttaat cactcaacgt aagtttgata atttaacgaa agctgaacgt ggaggtttga 3661 gtgaacttga taaagctggt tttatcaaac gccaattggt tgaaactcgc caaatcacta 3721 agcatgtggc acaaattttg gatagtcgca tgaatactaa atacgatgaa aatgataaac 3781 ttattcgaga ggttaaagtg attaccttaa aatctaaatt agtttctgac ttccgaaaag 3841 atttccaatt ctataaagta cgtgagatta acaattacca tcatgcccat gatgcgtatc 3901 taaatgccgt cgttggaact gctttgatta agaaatatcc aaaacttgaa tcggagtttg 3961 tctatggtga ttataaagtt tatgatgttc gtaaaatgat tgctaagtct gagcaagaaa 4021 taggcaaagc aaccgcaaaa tatttctttt actctaatat catgaacttc ttcaaaacag 4081 aaattacact tgcaaatgga gagattcgca aacgccctct aatcgaaact aatggggaaa 4141 ctggagaaat tgtctgggat aaagggcgag attttgccac agtgcgcaaa gtattgtcca 4201 tgccccaagt caatattgtc aagaaaacag aagtacagac aggcggattc tccaaggagt 4261 caattttacc aaaaagaaat tcggacaagc ttattgctcg taaaaaagac tgggatccaa 4321 aaaaatatgg tggttttgat agtccaacgg tagcttattc agtcctagtg gttgctaagg 4381 tggaaaaagg gaaatcgaag aagttaaaat ccgttaaaga gttactaggg atcacaatta 4441 tggaaagaag ttcctttgaa aaaaatccga ttgacttttt agaagctaaa ggatataagg 4501 aagttaaaaa agacttaatc attaaactac ctaaatatag tctttttgag ttagaaaacg 4561 gtcgtaaacg gatgctggct agtgccggag aattacaaaa aggaaatgag ctggctctgc 4621 caagcaaata tgtgaatttt ttatatttag ctagtcatta tgaaaagttg aagggtagtc 4681 cagaagataa cgaacaaaaa caattgtttg tggagcagca taagcattat ttagatgaga 4741 ttattgagca aatcagtgaa ttttctaagc gtgttatttt agcagatgcc aatttagata 4801 aagttcttag tgcatataac aaacatagag acaaaccaat acgtgaacaa gcagaaaata 4861 ttattcattt atttacgttg acgaatcttg gagctcccgc tgcttttaaa tattttgata 4921 caacaattga tcgtaaacga tatacgtcta caaaagaagt tttagatgcc actcttatcc 4981 atcaatccat cactggtctt tatgaaacac gcattgattt gagtcagcta ggaggtgact 5041 ga //