BBa_K1994003 1 BBa_K1994003 Superfolder GFP 2016-09-21T11:00:00Z 2016-09-22T03:14:22Z Synthetic vector pET-T7p(-12T)-SpacerA-GFP-LVA Superfolder GFP. 488nm Excitation, 530nm Emission false false _2461_ 27126 27126 9 false No special design considerations. false Liam Carroll BBa_K1994004 1 BBa_K1994004 RBS 28.4k_NEQ RBSCv1.1 2016-09-21T11:00:00Z 2016-10-19T12:05:49Z RBS 28.4k_NEQ RBSCv1.1 RBS used for superfolder GFP in team Warwick 2016's project. false false _2461_ 30870 27126 9 false No special design considerations. false Liam Carroll annotation2521776 1 RBS 28.4k_NEQ RBSCv1.1 range2521776 1 18 47 annotation2521745 1 TGG (Shine-Dalgarno sequence) range2521745 1 2 11 BBa_K1994018 1 BBa_K1994018 E. coli PheA terminator 2016-09-25T11:00:00Z 2016-09-26T08:18:57Z Escherichia coli PheA terminator from E. coli false false _2461_ 27126 27126 9 false No special considerations false Liam Carroll BBa_K1994044 1 BBa_K1994044 dCas9 Promoter 2016-10-13T11:00:00Z 2016-10-19T02:12:18Z sgRNA with PAM-Proximal complementary sequence 5 sgRNA with PAM-Proximal complementary sequence 5 false false _2461_ 30870 28134 9 false sgRNA with PAM-Proximal complementary sequence 5 false Egheosa Ogbomo annotation2522296 1 dCas9 Promoter range2522296 1 18 48 annotation2522272 1 Spacer range2522272 1 1 17 BBa_K1994000 1 BBa_K1994000 dCas9 enzyme 2016-09-21T11:00:00Z 2016-09-22T04:26:59Z Streptococcus Pyrogenes Part containing only dCas9 enzyme with no terminator or promoter. Interacts with sgRNA to bind to specific DNA target. false false _2461_ 27126 27126 9 false No special design considerations. false Liam Carroll BBa_K1994002 1 BBa_K1994002 RBS 173k TIRv1 2016-09-21T11:00:00Z 2016-09-22T04:24:08Z RBS 173k TIRv1 RBS used for dCas9 enzyme in team Warwick 2016's project. false false _2461_ 27126 27126 9 false No special design considerations. false Liam Carroll BBa_K1994060 1 BBa_K1994060 Multiple dCas9 binding sites with weak sigma 54 promoter upstream of GFP + dCas9 expression cassette 2016-10-13T11:00:00Z 2016-10-19T04:41:03Z dCas9 with Pam staggered sequence and sigma 54 promoter dCas9 with Pam staggered sequence and sigma 54 promoter false false _2461_ 30870 28134 9 false dCas9 with Pam staggered sequence and sigma 54 promoter false Egheosa Ogbomo component2522720 1 BBa_K1994002 component2522721 1 BBa_K1994000 component2522716 1 BBa_K1994018 component2522715 1 BBa_K1994003 component2522719 1 BBa_K1994044 component2522714 1 BBa_K1994004 annotation2522715 1 BBa_K1994003 range2522715 1 54 776 annotation2522714 1 BBa_K1994004 range2522714 1 1 47 annotation2522720 1 BBa_K1994002 range2522720 1 901 930 annotation2522719 1 BBa_K1994044 range2522719 1 845 892 annotation2522716 1 BBa_K1994018 range2522716 1 785 836 annotation2522721 1 BBa_K1994000 range2522721 1 939 5042 BBa_K1994002_sequence 1 caagggacatcaggaggagggaattataga BBa_K1994004_sequence 1 cttggtattatgctagctactagacaattaatagggaggttctcgag BBa_K1994000_sequence 1 gataagaaatactcaataggcttagctatcggcacaaatagcgtcggatgggcggtgatcactgatgaatataaggttccgtctaaaaagttcaaggttctgggaaatacagaccgccacagtatcaaaaaaaatcttataggggctcttttatttgacagtggagagacagcggaagcgactcgtctcaaacggacagctcgtagaaggtatacacgtcggaagaatcgtatttgttatctacaggagattttttcaaatgagatggcgaaagtagatgatagtttctttcatcgacttgaagagtcttttttggtggaagaagacaagaagcatgaacgtcatcctatttttggaaatatagtagatgaagttgcttatcatgagaaatatccaactatctatcatctgcgaaaaaaattggtagattctactgataaagcggatttgcgcttaatctatttggccttagcgcatatgattaagtttcgtggtcattttttgattgagggagatttaaatcctgataatagtgatgtggacaaactatttatccagttggtacaaacctacaatcaattatttgaagaaaaccctattaacgcaagtggagtagatgctaaagcgattctttctgcacgattgagtaaatcaagacgattagaaaatctcattgctcagctccccggtgagaagaaaaatggcttatttgggaatctcattgctttgtcattgggtttgacccctaattttaaatcaaattttgatttggcagaagatgctaaattacagctttcaaaagatacttacgatgatgatttagataatttattggcgcaaattggagatcaatatgctgatttgtttttggcagctaagaatttatcagatgctattttactttcagatatcctaagagtaaatactgaaataactaaggctcccctatcagcttcaatgattaaacgctacgatgaacatcatcaagacttgactcttttaaaagctttagttcgacaacaacttccagaaaagtataaagaaatcttttttgatcaatcaaaaaacggatatgcaggttatattgatgggggagctagccaagaagaattttataaatttatcaaaccaattttagaaaaaatggatggtactgaggaattattggtgaaactaaatcgtgaagatttgctgcgcaagcaacggacctttgacaacggctctattccccatcaaattcacttgggtgagctgcatgctattttgagaagacaagaagacttttatccatttttaaaagacaatcgtgagaagattgaaaaaatcttgacttttcgtattccttattatgttggtccattggcgcgtggcaatagtcgttttgcatggatgactcggaagtctgaagaaacaattaccccatggaattttgaagaagttgtcgataaaggtgcttcagctcaatcatttattgaacgcatgacaaactttgataaaaatcttccaaatgaaaaagtactaccaaaacatagtttgctttatgagtattttacggtttataacgaattgacaaaggtcaaatatgttactgaaggaatgcgaaaaccagcatttctttcaggtgaacagaagaaagccattgttgatttactcttcaaaacaaatcgaaaagtaaccgttaagcaattaaaagaagattatttcaaaaaaatagaatgttttgatagtgttgaaatttcaggagttgaagatagatttaatgcttcattaggtacctaccatgatttgctaaaaattattaaagataaagattttttggataatgaagaaaatgaagatatcttagaggatattgttttaacattgaccttatttgaagatagggagatgattgaggaaagacttaaaacatatgctcacctctttgatgataaggtgatgaaacagcttaaacgtcgccgttatactggttggggacgtttgtctcgaaaattgattaatggtattagggataagcaatctggcaaaacaatattagattttttgaaatcagatggttttgccaatcgcaattttatgcagctgatccatgatgatagtttgacatttaaagaagacattcaaaaagcacaagtgtctggacaaggcgatagtttacatgaacatattgcaaatttagctggtagccctgctattaaaaaaggtattttacagactgtaaaagttgttgatgaattggtcaaagtaatggggcggcataagccagaaaatatcgttattgaaatggcacgtgaaaatcagacaactcaaaagggccagaaaaattcgcgagagcgtatgaaacgaatcgaagaaggtatcaaagaattaggaagtcagattcttaaagagcatcctgttgaaaatactcaattgcaaaatgaaaagctctatctctattatctccaaaatggaagagacatgtatgtggaccaagaattagatattaatcgtttaagtgattatgatgtcgatgccattgttccacaaagtttccttaaagacgattcaatagacaataaggtcttaacgcgttctgataaaaatcgtggtaaatcggataacgttccaagtgaagaagtagtcaaaaagatgaaaaactattggagacaacttctaaacgccaagttaatcactcaacgtaagtttgataatttaacgaaagctgaacgtggaggtttgagtgaacttgataaagctggttttatcaaacgccaattggttgaaactcgccaaatcactaagcatgtggcacaaattttggatagtcgcatgaatactaaatacgatgaaaatgataaacttattcgagaggttaaagtgattaccttaaaatctaaattagtttctgacttccgaaaagatttccaattctataaagtacgtgagattaacaattaccatcatgcccatgatgcgtatctaaatgccgtcgttggaactgctttgattaagaaatatccaaaacttgaatcggagtttgtctatggtgattataaagtttatgatgttcgtaaaatgattgctaagtctgagcaagaaataggcaaagcaaccgcaaaatatttcttttactctaatatcatgaacttcttcaaaacagaaattacacttgcaaatggagagattcgcaaacgccctctaatcgaaactaatggggaaactggagaaattgtctgggataaagggcgagattttgccacagtgcgcaaagtattgtccatgccccaagtcaatattgtcaagaaaacagaagtacagacaggcggattctccaaggagtcaattttaccaaaaagaaattcggacaagcttattgctcgtaaaaaagactgggatccaaaaaaatatggtggttttgatagtccaacggtagcttattcagtcctagtggttgctaaggtggaaaaagggaaatcgaagaagttaaaatccgttaaagagttactagggatcacaattatggaaagaagttcctttgaaaaaaatccgattgactttttagaagctaaaggatataaggaagttaaaaaagacttaatcattaaactacctaaatatagtctttttgagttagaaaacggtcgtaaacggatgctggctagtgccggagaattacaaaaaggaaatgagctggctctgccaagcaaatatgtgaattttttatatttagctagtcattatgaaaagttgaagggtagtccagaagataacgaacaaaaacaattgtttgtggagcagcataagcattatttagatgagattattgagcaaatcagtgaattttctaagcgtgttattttagcagatgccaatttagataaagttcttagtgcatataacaaacatagagacaaaccaatacgtgaacaagcagaaaatattattcatttatttacgttgacgaatcttggagctcccgctgcttttaaatattttgatacaacaattgatcgtaaacgatatacgtctacaaaagaagttttagatgccactcttatccatcaatccatcactggtctttatgaaacacgcattgatttgagtcagctaggaggtgactga BBa_K1994018_sequence 1 gacgaacaataaggcctcccaaatcggggggccttttttattgataacaaaa BBa_K1994060_sequence 1 cttggtattatgctagctactagacaattaatagggaggttctcgagtactagatgcgtaaaggcgaagaactgtttaccggtgtggttccgattctggtggaactggacggcgatgttaatggtcataaattcagtgttcgcggcgaaggtgaaggcgatgcgacgaacggcaaactgaccctgaaatttatctgcaccacgggtaaactgccggtcccgtggccgacgctggtgaccacgctgacctatggcgttcaatgttttgcgcgttacccggatcacatgaaacagcacgactttttcaaatcggccatgccggaaggctatgtgcaggaacgtacgattagctttaaagacgatggtacgtataaaacccgcgcggaagtgaaattcgaaggcgataccctggttaaccgtatcgaactgaaaggtatcgatttcaaagaagacggcaatattctgggtcataaactggaatataacttcaattcccacaacgtgtacatcaccgcggataaacagaaaaacggcattaaagccaatttcaaaatccgccataatgtggaagatggtagcgttcagctggccgaccactatcagcaaaacacgccgattggtgatggcccggtcctgctgccggacaatcactacctgagtacccagtccgtgctgtcaaaagatccgaacgaaaaacgtgaccacatggtcctgctggaatttgtgacggctgcgggtatcacccacggcatggacgaactgtataaaaggccttaatactagaggacgaacaataaggcctcccaaatcggggggccttttttattgataacaaaatactagagcctaggccaggcatcaattgaaatcatcaaactcattatggatttaattactagagcaagggacatcaggaggagggaattatagatactagaggataagaaatactcaataggcttagctatcggcacaaatagcgtcggatgggcggtgatcactgatgaatataaggttccgtctaaaaagttcaaggttctgggaaatacagaccgccacagtatcaaaaaaaatcttataggggctcttttatttgacagtggagagacagcggaagcgactcgtctcaaacggacagctcgtagaaggtatacacgtcggaagaatcgtatttgttatctacaggagattttttcaaatgagatggcgaaagtagatgatagtttctttcatcgacttgaagagtcttttttggtggaagaagacaagaagcatgaacgtcatcctatttttggaaatatagtagatgaagttgcttatcatgagaaatatccaactatctatcatctgcgaaaaaaattggtagattctactgataaagcggatttgcgcttaatctatttggccttagcgcatatgattaagtttcgtggtcattttttgattgagggagatttaaatcctgataatagtgatgtggacaaactatttatccagttggtacaaacctacaatcaattatttgaagaaaaccctattaacgcaagtggagtagatgctaaagcgattctttctgcacgattgagtaaatcaagacgattagaaaatctcattgctcagctccccggtgagaagaaaaatggcttatttgggaatctcattgctttgtcattgggtttgacccctaattttaaatcaaattttgatttggcagaagatgctaaattacagctttcaaaagatacttacgatgatgatttagataatttattggcgcaaattggagatcaatatgctgatttgtttttggcagctaagaatttatcagatgctattttactttcagatatcctaagagtaaatactgaaataactaaggctcccctatcagcttcaatgattaaacgctacgatgaacatcatcaagacttgactcttttaaaagctttagttcgacaacaacttccagaaaagtataaagaaatcttttttgatcaatcaaaaaacggatatgcaggttatattgatgggggagctagccaagaagaattttataaatttatcaaaccaattttagaaaaaatggatggtactgaggaattattggtgaaactaaatcgtgaagatttgctgcgcaagcaacggacctttgacaacggctctattccccatcaaattcacttgggtgagctgcatgctattttgagaagacaagaagacttttatccatttttaaaagacaatcgtgagaagattgaaaaaatcttgacttttcgtattccttattatgttggtccattggcgcgtggcaatagtcgttttgcatggatgactcggaagtctgaagaaacaattaccccatggaattttgaagaagttgtcgataaaggtgcttcagctcaatcatttattgaacgcatgacaaactttgataaaaatcttccaaatgaaaaagtactaccaaaacatagtttgctttatgagtattttacggtttataacgaattgacaaaggtcaaatatgttactgaaggaatgcgaaaaccagcatttctttcaggtgaacagaagaaagccattgttgatttactcttcaaaacaaatcgaaaagtaaccgttaagcaattaaaagaagattatttcaaaaaaatagaatgttttgatagtgttgaaatttcaggagttgaagatagatttaatgcttcattaggtacctaccatgatttgctaaaaattattaaagataaagattttttggataatgaagaaaatgaagatatcttagaggatattgttttaacattgaccttatttgaagatagggagatgattgaggaaagacttaaaacatatgctcacctctttgatgataaggtgatgaaacagcttaaacgtcgccgttatactggttggggacgtttgtctcgaaaattgattaatggtattagggataagcaatctggcaaaacaatattagattttttgaaatcagatggttttgccaatcgcaattttatgcagctgatccatgatgatagtttgacatttaaagaagacattcaaaaagcacaagtgtctggacaaggcgatagtttacatgaacatattgcaaatttagctggtagccctgctattaaaaaaggtattttacagactgtaaaagttgttgatgaattggtcaaagtaatggggcggcataagccagaaaatatcgttattgaaatggcacgtgaaaatcagacaactcaaaagggccagaaaaattcgcgagagcgtatgaaacgaatcgaagaaggtatcaaagaattaggaagtcagattcttaaagagcatcctgttgaaaatactcaattgcaaaatgaaaagctctatctctattatctccaaaatggaagagacatgtatgtggaccaagaattagatattaatcgtttaagtgattatgatgtcgatgccattgttccacaaagtttccttaaagacgattcaatagacaataaggtcttaacgcgttctgataaaaatcgtggtaaatcggataacgttccaagtgaagaagtagtcaaaaagatgaaaaactattggagacaacttctaaacgccaagttaatcactcaacgtaagtttgataatttaacgaaagctgaacgtggaggtttgagtgaacttgataaagctggttttatcaaacgccaattggttgaaactcgccaaatcactaagcatgtggcacaaattttggatagtcgcatgaatactaaatacgatgaaaatgataaacttattcgagaggttaaagtgattaccttaaaatctaaattagtttctgacttccgaaaagatttccaattctataaagtacgtgagattaacaattaccatcatgcccatgatgcgtatctaaatgccgtcgttggaactgctttgattaagaaatatccaaaacttgaatcggagtttgtctatggtgattataaagtttatgatgttcgtaaaatgattgctaagtctgagcaagaaataggcaaagcaaccgcaaaatatttcttttactctaatatcatgaacttcttcaaaacagaaattacacttgcaaatggagagattcgcaaacgccctctaatcgaaactaatggggaaactggagaaattgtctgggataaagggcgagattttgccacagtgcgcaaagtattgtccatgccccaagtcaatattgtcaagaaaacagaagtacagacaggcggattctccaaggagtcaattttaccaaaaagaaattcggacaagcttattgctcgtaaaaaagactgggatccaaaaaaatatggtggttttgatagtccaacggtagcttattcagtcctagtggttgctaaggtggaaaaagggaaatcgaagaagttaaaatccgttaaagagttactagggatcacaattatggaaagaagttcctttgaaaaaaatccgattgactttttagaagctaaaggatataaggaagttaaaaaagacttaatcattaaactacctaaatatagtctttttgagttagaaaacggtcgtaaacggatgctggctagtgccggagaattacaaaaaggaaatgagctggctctgccaagcaaatatgtgaattttttatatttagctagtcattatgaaaagttgaagggtagtccagaagataacgaacaaaaacaattgtttgtggagcagcataagcattatttagatgagattattgagcaaatcagtgaattttctaagcgtgttattttagcagatgccaatttagataaagttcttagtgcatataacaaacatagagacaaaccaatacgtgaacaagcagaaaatattattcatttatttacgttgacgaatcttggagctcccgctgcttttaaatattttgatacaacaattgatcgtaaacgatatacgtctacaaaagaagttttagatgccactcttatccatcaatccatcactggtctttatgaaacacgcattgatttgagtcagctaggaggtgactga BBa_K1994003_sequence 1 atgcgtaaaggcgaagaactgtttaccggtgtggttccgattctggtggaactggacggcgatgttaatggtcataaattcagtgttcgcggcgaaggtgaaggcgatgcgacgaacggcaaactgaccctgaaatttatctgcaccacgggtaaactgccggtcccgtggccgacgctggtgaccacgctgacctatggcgttcaatgttttgcgcgttacccggatcacatgaaacagcacgactttttcaaatcggccatgccggaaggctatgtgcaggaacgtacgattagctttaaagacgatggtacgtataaaacccgcgcggaagtgaaattcgaaggcgataccctggttaaccgtatcgaactgaaaggtatcgatttcaaagaagacggcaatattctgggtcataaactggaatataacttcaattcccacaacgtgtacatcaccgcggataaacagaaaaacggcattaaagccaatttcaaaatccgccataatgtggaagatggtagcgttcagctggccgaccactatcagcaaaacacgccgattggtgatggcccggtcctgctgccggacaatcactacctgagtacccagtccgtgctgtcaaaagatccgaacgaaaaacgtgaccacatggtcctgctggaatttgtgacggctgcgggtatcacccacggcatggacgaactgtataaaaggccttaa BBa_K1994044_sequence 1 cctaggccaggcatcaattgaaatcatcaaactcattatggatttaat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z