##gff-version 3
##sequence-region BBa_K1994060 1 5042
BBa_K1994060	.	ribosome_entry_site	1	47	.	+	0	ID=BBa_K1994004;Name=BBa_K1994004
BBa_K1994060	.	sequence_feature	2	11	.	+	0	ID=annotation2521745;Name=TGG (Shine-Dalgarno sequence);Parent=BBa_K1994004
BBa_K1994060	.	ribosome_entry_site	18	47	.	+	0	ID=annotation2521776;Name=RBS 28.4k_NEQ RBSCv1.1;Parent=BBa_K1994004
BBa_K1994060	.	CDS	939	5042	.	+	0	ID=BBa_K1994000;Name=BBa_K1994000
BBa_K1994060	.	terminator	785	836	.	+	0	ID=BBa_K1994018;Name=BBa_K1994018
BBa_K1994060	.	engineered_region	54	776	.	+	0	ID=BBa_K1994003;Name=BBa_K1994003
BBa_K1994060	.	ribosome_entry_site	901	930	.	+	0	ID=BBa_K1994002;Name=BBa_K1994002
BBa_K1994060	.	promoter	845	892	.	+	0	ID=BBa_K1994044;Name=BBa_K1994044
BBa_K1994060	.	sequence_feature	845	861	.	+	0	ID=annotation2522272;Name=Spacer;Parent=BBa_K1994044
BBa_K1994060	.	promoter	862	892	.	+	0	ID=annotation2522296;Name=dCas9 Promoter;Parent=BBa_K1994044
>BBa_K1994060
cttggtattatgctagctactagacaattaatagggaggttctcgagtactagatgcgtaaaggcgaagaactgtttac
cggtgtggttccgattctggtggaactggacggcgatgttaatggtcataaattcagtgttcgcggcgaaggtgaaggc
gatgcgacgaacggcaaactgaccctgaaatttatctgcaccacgggtaaactgccggtcccgtggccgacgctggtga
ccacgctgacctatggcgttcaatgttttgcgcgttacccggatcacatgaaacagcacgactttttcaaatcggccat
gccggaaggctatgtgcaggaacgtacgattagctttaaagacgatggtacgtataaaacccgcgcggaagtgaaattc
gaaggcgataccctggttaaccgtatcgaactgaaaggtatcgatttcaaagaagacggcaatattctgggtcataaac
tggaatataacttcaattcccacaacgtgtacatcaccgcggataaacagaaaaacggcattaaagccaatttcaaaat
ccgccataatgtggaagatggtagcgttcagctggccgaccactatcagcaaaacacgccgattggtgatggcccggtc
ctgctgccggacaatcactacctgagtacccagtccgtgctgtcaaaagatccgaacgaaaaacgtgaccacatggtcc
tgctggaatttgtgacggctgcgggtatcacccacggcatggacgaactgtataaaaggccttaatactagaggacgaa
caataaggcctcccaaatcggggggccttttttattgataacaaaatactagagcctaggccaggcatcaattgaaatc
atcaaactcattatggatttaattactagagcaagggacatcaggaggagggaattatagatactagaggataagaaat
actcaataggcttagctatcggcacaaatagcgtcggatgggcggtgatcactgatgaatataaggttccgtctaaaaa
gttcaaggttctgggaaatacagaccgccacagtatcaaaaaaaatcttataggggctcttttatttgacagtggagag
acagcggaagcgactcgtctcaaacggacagctcgtagaaggtatacacgtcggaagaatcgtatttgttatctacagg
agattttttcaaatgagatggcgaaagtagatgatagtttctttcatcgacttgaagagtcttttttggtggaagaaga
caagaagcatgaacgtcatcctatttttggaaatatagtagatgaagttgcttatcatgagaaatatccaactatctat
catctgcgaaaaaaattggtagattctactgataaagcggatttgcgcttaatctatttggccttagcgcatatgatta
agtttcgtggtcattttttgattgagggagatttaaatcctgataatagtgatgtggacaaactatttatccagttggt
acaaacctacaatcaattatttgaagaaaaccctattaacgcaagtggagtagatgctaaagcgattctttctgcacga
ttgagtaaatcaagacgattagaaaatctcattgctcagctccccggtgagaagaaaaatggcttatttgggaatctca
ttgctttgtcattgggtttgacccctaattttaaatcaaattttgatttggcagaagatgctaaattacagctttcaaa
agatacttacgatgatgatttagataatttattggcgcaaattggagatcaatatgctgatttgtttttggcagctaag
aatttatcagatgctattttactttcagatatcctaagagtaaatactgaaataactaaggctcccctatcagcttcaa
tgattaaacgctacgatgaacatcatcaagacttgactcttttaaaagctttagttcgacaacaacttccagaaaagta
taaagaaatcttttttgatcaatcaaaaaacggatatgcaggttatattgatgggggagctagccaagaagaattttat
aaatttatcaaaccaattttagaaaaaatggatggtactgaggaattattggtgaaactaaatcgtgaagatttgctgc
gcaagcaacggacctttgacaacggctctattccccatcaaattcacttgggtgagctgcatgctattttgagaagaca
agaagacttttatccatttttaaaagacaatcgtgagaagattgaaaaaatcttgacttttcgtattccttattatgtt
ggtccattggcgcgtggcaatagtcgttttgcatggatgactcggaagtctgaagaaacaattaccccatggaattttg
aagaagttgtcgataaaggtgcttcagctcaatcatttattgaacgcatgacaaactttgataaaaatcttccaaatga
aaaagtactaccaaaacatagtttgctttatgagtattttacggtttataacgaattgacaaaggtcaaatatgttact
gaaggaatgcgaaaaccagcatttctttcaggtgaacagaagaaagccattgttgatttactcttcaaaacaaatcgaa
aagtaaccgttaagcaattaaaagaagattatttcaaaaaaatagaatgttttgatagtgttgaaatttcaggagttga
agatagatttaatgcttcattaggtacctaccatgatttgctaaaaattattaaagataaagattttttggataatgaa
gaaaatgaagatatcttagaggatattgttttaacattgaccttatttgaagatagggagatgattgaggaaagactta
aaacatatgctcacctctttgatgataaggtgatgaaacagcttaaacgtcgccgttatactggttggggacgtttgtc
tcgaaaattgattaatggtattagggataagcaatctggcaaaacaatattagattttttgaaatcagatggttttgcc
aatcgcaattttatgcagctgatccatgatgatagtttgacatttaaagaagacattcaaaaagcacaagtgtctggac
aaggcgatagtttacatgaacatattgcaaatttagctggtagccctgctattaaaaaaggtattttacagactgtaaa
agttgttgatgaattggtcaaagtaatggggcggcataagccagaaaatatcgttattgaaatggcacgtgaaaatcag
acaactcaaaagggccagaaaaattcgcgagagcgtatgaaacgaatcgaagaaggtatcaaagaattaggaagtcaga
ttcttaaagagcatcctgttgaaaatactcaattgcaaaatgaaaagctctatctctattatctccaaaatggaagaga
catgtatgtggaccaagaattagatattaatcgtttaagtgattatgatgtcgatgccattgttccacaaagtttcctt
aaagacgattcaatagacaataaggtcttaacgcgttctgataaaaatcgtggtaaatcggataacgttccaagtgaag
aagtagtcaaaaagatgaaaaactattggagacaacttctaaacgccaagttaatcactcaacgtaagtttgataattt
aacgaaagctgaacgtggaggtttgagtgaacttgataaagctggttttatcaaacgccaattggttgaaactcgccaa
atcactaagcatgtggcacaaattttggatagtcgcatgaatactaaatacgatgaaaatgataaacttattcgagagg
ttaaagtgattaccttaaaatctaaattagtttctgacttccgaaaagatttccaattctataaagtacgtgagattaa
caattaccatcatgcccatgatgcgtatctaaatgccgtcgttggaactgctttgattaagaaatatccaaaacttgaa
tcggagtttgtctatggtgattataaagtttatgatgttcgtaaaatgattgctaagtctgagcaagaaataggcaaag
caaccgcaaaatatttcttttactctaatatcatgaacttcttcaaaacagaaattacacttgcaaatggagagattcg
caaacgccctctaatcgaaactaatggggaaactggagaaattgtctgggataaagggcgagattttgccacagtgcgc
aaagtattgtccatgccccaagtcaatattgtcaagaaaacagaagtacagacaggcggattctccaaggagtcaattt
taccaaaaagaaattcggacaagcttattgctcgtaaaaaagactgggatccaaaaaaatatggtggttttgatagtcc
aacggtagcttattcagtcctagtggttgctaaggtggaaaaagggaaatcgaagaagttaaaatccgttaaagagtta
ctagggatcacaattatggaaagaagttcctttgaaaaaaatccgattgactttttagaagctaaaggatataaggaag
ttaaaaaagacttaatcattaaactacctaaatatagtctttttgagttagaaaacggtcgtaaacggatgctggctag
tgccggagaattacaaaaaggaaatgagctggctctgccaagcaaatatgtgaattttttatatttagctagtcattat
gaaaagttgaagggtagtccagaagataacgaacaaaaacaattgtttgtggagcagcataagcattatttagatgaga
ttattgagcaaatcagtgaattttctaagcgtgttattttagcagatgccaatttagataaagttcttagtgcatataa
caaacatagagacaaaccaatacgtgaacaagcagaaaatattattcatttatttacgttgacgaatcttggagctccc
gctgcttttaaatattttgatacaacaattgatcgtaaacgatatacgtctacaaaagaagttttagatgccactctta
tccatcaatccatcactggtctttatgaaacacgcattgatttgagtcagctaggaggtgactga