LOCUS       BBa_K1997011            2019 bp    DNA     linear       22-Nov-2024
DEFINITION  P+R->sHRP-N->FRB->RBS->FKBP->sHRP-C->Ter
ACCESSION   BBa_K1997011
VERSION     BBa_K1997011.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..223
                     /label=RBS30
     RBS             209..223
                     /label=BBa_K1789002
     misc_feature    1..223
                     /label=BBa_K1789012
     CDS             230..870
                     /label=sHRP-N
     CDS             871..900
                     /label=10aa linker
     CDS             913..1197
                     /label=FRB
     RBS             1198..1227
                     /label=RBS
     CDS             1228..1551
                     /label=FKBP
     CDS             1564..1593
                     /label=10aa linker
     CDS             1594..1882
                     /label=sHRP-C
     misc_feature    230..1882
                     /label=BBa_K1997005
     stem_loop       1902..1945
                     /label=stem_loop
     misc_feature    1891..1970
                     /label=BBa_B0010
     terminator      1891..1970
                     /label=BBa_B0010
     stem_loop       1986..2005
                     /label=T7 TE
     misc_feature    2012..2012
                     /label=stop
     polyA_site      2006..2019
                     /label=polya
     misc_feature    1979..2019
                     /label=BBa_B0012
     terminator      1979..2019
                     /label=BBa_B0012
     terminator      1891..2019
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tgcagttaac
      241 ccctacattc tacgacaata gctgtcccaa cgtgtccaac atcgttcgcg acattatcgt
      301 caacgagctc agatccgatc ccaggatcgc tgcttcaata ttacgtctgc acttccatga
      361 ctgcttcgtg aatggttgcg acgctagcat attactggac aacaccacca gtttccgcac
      421 tgaaaaggat gcattcggga acgctaacag cgccaggggc tttagcgtga tcgatcgcat
      481 gaaggctgcc gttgagtcag catgcccagg tacagtcagt tgtgcagacc tgctgactat
      541 agctgcgcaa cagagcgtga ctcttgcagg cggaccgtcc tggagagtgc cgctcggtcg
      601 acgtgactcc ctacaggcat tcctagatct ggccaacgcc aacttgcctg ctccattctt
      661 caccctgccc cagctgaagg atagctttag aaacgtgggt ctgaatcgct caagtgacct
      721 tgtggctctg tccggaggac acacatttgg aaagagccag tgtaggttca tcatggatag
      781 gctctacaat ttcagcaaca ctgggttacc tgaccccacg ctgaacacta cgtatctcca
      841 gacactgaga ggcttgtgcc cactgaatgg cggtggttct ggtggtggtt ctggtggttc
      901 tataggcgag accatgatcc tctggcatga gatgtggcat gaaggcctgg aagaggcatc
      961 tcgtttgtac tttggggaaa ggaacgtgaa aggcatgttt gaggtgctgg agcccttgca
     1021 tgctatgatg gaacggggcc cccagactct gaaggaaaca tcctttaatc aggcctatgg
     1081 tcgagattta atggaggccc aagagtggtg caggaagtac atgaaatcag ggaatgtcaa
     1141 ggacctcacc caagcctggg acctctatta tcatgtgttc cgacgaatct catgataaac
     1201 ctaactaagt aattaaagga aacagacgat gggagtgcag gtggaaacca tctccccagg
     1261 agacgggcgc accttcccca agcgcggcca gacctgcgtg gtgcactaca ccgggatgct
     1321 tgaagatgga aagaaatttg attcctcccg ggacagaaac aagcccttta agtttatgct
     1381 aggcaagcag gaggtgatcc gaggctggga agaaggggtt gcccagatga gtgtgggtca
     1441 gagagccaaa ctgactatat ctccagatta tgcctatggt gccactgggc acccaggcat
     1501 catcccacca catgccactc tcgtcttcga tgtggagctt ctaaaactgg aaggtctcag
     1561 tttaggttct ggtggtggtt ctggtggtgg ttctaacctc agtgcactgg tggactttga
     1621 tctgcggacc ccaaccatct tcgataacaa gtactatgtg aatttagaag agcagaaagg
     1681 cctgatacag agtgatcaag aactgtttag cagtccagat gccactgaca ccatcccact
     1741 ggtgagaagt tttgctaact ctactcaaac cttctttaac gccttcgtgg aagccatgga
     1801 ccgtatgggt aacattaccc ctctgacggg tacccaaggc cagattcgtc gcaactgtag
     1861 agtggtcaac agcaactctt aatactagag ccaggcatca aataaaacga aaggctcagt
     1921 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     1981 acactggctc accttcgggt gggcctttct gcgtttata
//