BBa_K1997012
1
BBa_K1997012
P+R->sHRP-N->Zif268-> sHRP-C->TER
2016-10-12T11:00:00Z
2016-10-13T06:18:45Z
This part was synthesized by IDT
This part is an integrated tool for protein-protein interaction research using split-HRP system as reporter. the "Zif268" subpart can be easily replaced using Golden Gate technique with BsaI
false
false
_2464_
26296
26296
9
false
We added BsaI sites at the flank of "Zif268" sequence so that this sequence can be easily replaced
false
Chushu Zhu
annotation2500094
1
BBa_K1789012
range2500094
1
1
223
annotation2500109
1
10aa linker
range2500109
1
871
900
annotation2500112
1
sHRP-C
range2500112
1
1216
1503
annotation2500114
1
BBa_B0015
range2500114
1
1510
1638
annotation2500113
1
barcode
range2500113
1
1504
1509
annotation2500095
1
barcode
range2500095
1
224
228
annotation2500111
1
10aa linker
range2500111
1
1186
1215
annotation2500110
1
Zif268
range2500110
1
913
1173
annotation2500096
1
sHRP-N
range2500096
1
229
870
BBa_K1997012_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaaactagatgcagttgacaccaacgttctatgacaattcctgcccaaacgtctctaatatcgttcgtgatatcattgtcaatgagctgcgttcggacccccgcattgctgcgtctatcctgcgtctgcattttcacgattgctttgtcaacggatgtgacgcttctattttattagataacacgacatcatttcgcaccgaaaaagatgcttttgggaatgcaaactcggcacgtgggttctcggtcattgaccgtatgaaggcggcagtcgagtctgcatgtccagggactgtgtcgtgtgccgaccttcttaccatcgcggcgcaacagtccgttactttagctgggggaccatcgtggcgcgtaccattgggccgtcgcgattccctgcaagcattcctggatttagcaaacgctaacctgcccgcccctttctttaccttaccccaattaaaggactcgttccgcaacgtgggattaaatcgtagcagtgacttagtagcccttagtggcggtcacaccttcggtaagtcacaatgccgcttcattatggaccgtctttataatttcagcaatacaggtttacctgaccccacgttaaacactacttatttgcaaaccttgcgtggcctttgtccgttaaacgggggagggagtggtggaggttccgggggaagtataggcgagaccccaggggaaaagccctacgcgtgccctgtagaatcgtgtgatcgccgcttttcacgcagtgacgagttaacacgtcacatccgtatccatacgggacaaaaaccttttcaatgtcgtatttgtatgcgtaatttctcacgttcagaccatctgaccacgcatatccgtactcatactggcgagaagccttttgcctgtgatatctgcggccgtaagttcgctcgttcagatgagcgtaagcgccatacaaagatccataccggtctcagtttaggttccggtggtggctccggcggcggcagcaatttatctgcattagttgattttgatttgcgtacgccgacaatcttcgacaacaaatactatgtgaatttggaagagcagaagggattaattcaatctgatcaggaacttttcagtagccccgacgccacagacacaatccctttggttcgctccttcgccaattctactcagacgtttttcaatgctttcgttgaagcaatggaccgtatggggaatattactccccttactgggacgcaggggcagattcgccgcaattgccgtgtcgttaatagtaactcgtaaactagaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z