BBa_K1997012 1 BBa_K1997012 P+R->sHRP-N->Zif268-> sHRP-C->TER 2016-10-12T11:00:00Z 2016-10-13T06:18:45Z This part was synthesized by IDT This part is an integrated tool for protein-protein interaction research using split-HRP system as reporter. the "Zif268" subpart can be easily replaced using Golden Gate technique with BsaI false false _2464_ 26296 26296 9 false We added BsaI sites at the flank of "Zif268" sequence so that this sequence can be easily replaced false Chushu Zhu annotation2500110 1 Zif268 range2500110 1 913 1173 annotation2500095 1 barcode range2500095 1 224 228 annotation2500114 1 BBa_B0015 range2500114 1 1510 1638 annotation2500096 1 sHRP-N range2500096 1 229 870 annotation2500109 1 10aa linker range2500109 1 871 900 annotation2500112 1 sHRP-C range2500112 1 1216 1503 annotation2500111 1 10aa linker range2500111 1 1186 1215 annotation2500113 1 barcode range2500113 1 1504 1509 annotation2500094 1 BBa_K1789012 range2500094 1 1 223 BBa_K1997012_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaaactagatgcagttgacaccaacgttctatgacaattcctgcccaaacgtctctaatatcgttcgtgatatcattgtcaatgagctgcgttcggacccccgcattgctgcgtctatcctgcgtctgcattttcacgattgctttgtcaacggatgtgacgcttctattttattagataacacgacatcatttcgcaccgaaaaagatgcttttgggaatgcaaactcggcacgtgggttctcggtcattgaccgtatgaaggcggcagtcgagtctgcatgtccagggactgtgtcgtgtgccgaccttcttaccatcgcggcgcaacagtccgttactttagctgggggaccatcgtggcgcgtaccattgggccgtcgcgattccctgcaagcattcctggatttagcaaacgctaacctgcccgcccctttctttaccttaccccaattaaaggactcgttccgcaacgtgggattaaatcgtagcagtgacttagtagcccttagtggcggtcacaccttcggtaagtcacaatgccgcttcattatggaccgtctttataatttcagcaatacaggtttacctgaccccacgttaaacactacttatttgcaaaccttgcgtggcctttgtccgttaaacgggggagggagtggtggaggttccgggggaagtataggcgagaccccaggggaaaagccctacgcgtgccctgtagaatcgtgtgatcgccgcttttcacgcagtgacgagttaacacgtcacatccgtatccatacgggacaaaaaccttttcaatgtcgtatttgtatgcgtaatttctcacgttcagaccatctgaccacgcatatccgtactcatactggcgagaagccttttgcctgtgatatctgcggccgtaagttcgctcgttcagatgagcgtaagcgccatacaaagatccataccggtctcagtttaggttccggtggtggctccggcggcggcagcaatttatctgcattagttgattttgatttgcgtacgccgacaatcttcgacaacaaatactatgtgaatttggaagagcagaagggattaattcaatctgatcaggaacttttcagtagccccgacgccacagacacaatccctttggttcgctccttcgccaattctactcagacgtttttcaatgctttcgttgaagcaatggaccgtatggggaatattactccccttactgggacgcaggggcagattcgccgcaattgccgtgtcgttaatagtaactcgtaaactagaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z