BBa_K1997014 1 BBa_K1997014 GFP10->Zif268->GFP11->RBS->GFP1-9 2016-10-12T11:00:00Z 2016-10-19T06:47:52Z This part was synthesized by IDT This part is an integrated tool for protein-protein interaction research using split-HRP system as reporter. the "FRB-RBS-FKBP" subpart can be easily replaced using Golden Gate technique with BsaI false false _2464_ 21134 21134 9 It's complicated true We added BsaI sites at the flank of "FRB-RBS-FKBP" sequence so that this sequence can be easily replaced false Xinyuan Qiu annotation2514072 1 GFP10 range2514072 1 1 60 annotation2514073 1 15aa linker range2514073 1 61 106 annotation2514074 1 Zif268 range2514074 1 119 378 annotation2514077 1 RBS range2514077 1 502 531 annotation2514078 1 GFP9 range2514078 1 532 1122 annotation2514075 1 15aa linker range2514075 1 391 435 annotation2514076 1 GFP11 range2514076 1 436 501 BBa_K1997014_sequence 1 atggatttaccagacgatcattacctgtccacacaaacaattctttcgaaagatcttaacggaactgacgttgggtctggcggtggttccggtggtggaccttctataggcgagaccccgggtgaaaaaccgtacgcttgcccggttgaatcttgcgaccgtcgtttctctcgttctgacgaactgacccgtcacatccgtatccacaccggtcagaaaccgttccagtgccgtatctgcatgcgtaacttctctcgttctgaccacctgaccacccacatccgtacccacaccggtgaaaaaccgttcgcttgcgacatctgcggtcgtaaattcgctcgttctgacgaacgtaaacgtcacaccaaaatccacaccaggtctcagtttggtggcggtggttcaggtggagggtctggtggcggatcaacatctgaaaagagagaccacatggtccttcttgagtatgtaacagctgctgggattacagatgccagttaaacctaactaagtaattaaaggaaacagacgatgcgtaaaggagaagaacttttcactggagttgtcccaattcttattgaattagatggtgatgttaatgggcacaaattttttgtcagaggagagggtgaaggtgatgcaacaaacggaaaacttagccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttaacctatggtgttcaatgctttagtagatacccagatcatatgaaacgccatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatatttcaaagatgacgggacatacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaaaatggaaacattcttggacacaaattggaatacaactttaactcacacaaagtatacatcaccgcagacaaacaaaacaatggaatcaaagcaaacttcacaattagacacaacgttgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z