LOCUS       BBa_K1997016            1461 bp    DNA     linear       26-Dec-2024
DEFINITION  P+R->sGFP-N->Zif-N->RBS->Zif-C->sGFP-C->TER
ACCESSION   BBa_K1997016
VERSION     BBa_K1997016.1  
FEATURES             Location/Qualifiers
     misc_feature    1..223
                     /label=	BBa_K1789012
     CDS             229..699
                     /label=sGFP-N
     CDS             700..729
                     /label=10aa linker
     CDS             742..930
                     /label=Zif-N
     RBS             931..960
                     /label=RBS
     CDS             961..1038
                     /label=	Zif-C
     CDS             1051..1080
                     /label=10aa linker
     CDS             1081..1326
                     /label=	sGFP-C
     misc_feature    1333..1461
                     /label=	BBa_B0015
     misc_feature    224..228
                     /label=	barcode
     misc_feature    1327..1332
                     /label=	barcode
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagat taaagaggag aaaactagat gcgtaaagga
      241 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
      301 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
      361 aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc
      421 ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc
      481 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg
      541 aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag
      601 ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac
      661 tataactcac acaatgtata catcatggca gacaaacaag gtggttctgg tggtggttct
      721 ggtggttcta taggcgagac cccgggtgaa aaaccgtacg cttgcccggt tgaatcttgc
      781 gaccgtcgtt tctctcgttc tgacgaactg acccgtcaca tccgtatcca caccggtcag
      841 aaaccgttcc agtgccgtat ctgcatgcgt aacttctctc gttctgacca cctgaccacc
      901 cacatccgta cccacaccgg tgaaaaataa acctaactaa gtaattaaag gaaacagacg
      961 atgccgttcg cttgcgacat ctgcggtcgt aaattcgctc gttctgacga acgtaaacgt
     1021 cacaccaaaa tccacaccgg tctcagttta ggttctggtg gtggttctgg tggtggttct
     1081 aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa
     1141 ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac
     1201 aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac
     1261 atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac
     1321 aaataaacta gaccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     1381 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     1441 gtgggccttt ctgcgtttat a
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