LOCUS       BBa_K1997017            1803 bp    DNA     linear       26-Dec-2024
DEFINITION  P+R->sGFP-N->FRB->RBS->FKBP ->sGFP-C->TER
ACCESSION   BBa_K1997017
VERSION     BBa_K1997017.1  
FEATURES             Location/Qualifiers
     misc_feature    1..223
                     /label=	BBa_K1789012
     misc_feature    224..228
                     /label=	barcode
     CDS             229..699
                     /label=	sGFP-N
     CDS             700..729
                     /label=	10aa linker
     CDS             742..1026
                     /label=	FRB
     RBS             1027..1056
                     /label=	RBS
     CDS             1057..1380
                     /label=	FKBP
     CDS             1393..1422
                     /label=	10aa linker
     CDS             1423..1668
                     /label=	sGFP-C
     misc_feature    1669..1674
                     /label=	barcode
     misc_feature    1675..1803
                     /label=	BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagat taaagaggag aaaactagat gcgtaaagga
      241 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
      301 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
      361 aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc
      421 ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc
      481 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg
      541 aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag
      601 ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac
      661 tataactcac acaatgtata catcatggca gacaaacaag gtggttctgg tggtggttct
      721 ggtggttcta taggcgagac catgatcctc tggcatgaga tgtggcatga aggcctggaa
      781 gaggcatctc gtttgtactt tggggaaagg aacgtgaaag gcatgtttga ggtgctggag
      841 cccttgcatg ctatgatgga acggggcccc cagactctga aggaaacatc ctttaatcag
      901 gcctatggtc gagatttaat ggaggcccaa gagtggtgca ggaagtacat gaaatcaggg
      961 aatgtcaagg acctcaccca agcctgggac ctctattatc atgtgttccg acgaatctca
     1021 tgataaacct aactaagtaa ttaaaggaaa cagacgatgg gagtgcaggt ggaaaccatc
     1081 tccccaggag acgggcgcac cttccccaag cgcggccaga cctgcgtggt gcactacacc
     1141 gggatgcttg aagatggaaa gaaatttgat tcctcccggg acagaaacaa gccctttaag
     1201 tttatgctag gcaagcagga ggtgatccga ggctgggaag aaggggttgc ccagatgagt
     1261 gtgggtcaga gagccaaact gactatatct ccagattatg cctatggtgc cactgggcac
     1321 ccaggcatca tcccaccaca tgccactctc gtcttcgatg tggagcttct aaaactggaa
     1381 ggtctcagtt taggttctgg tggtggttct ggtggtggtt ctaagaatgg aatcaaagtt
     1441 aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa
     1501 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca
     1561 caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt
     1621 gtaacagctg ctgggattac acatggcatg gatgaactat acaaataaac tagaccaggc
     1681 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1741 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     1801 ata
//