BBa_K1997017 1 BBa_K1997017 P+R->sGFP-N->FRB->RBS->FKBP ->sGFP-C->TER 2016-10-12T11:00:00Z 2016-10-13T06:51:33Z This part was synthesized by IDT This part is an integrated tool for protein-protein interaction research using split-GFP system as reporter. the "FRB-RBS-FKBP" subpart can be easily replaced using Golden Gate technique with BsaI false false _2464_ 26296 26296 9 false We added BsaI sites at the flank of "FRB-RBS-FKBP" sequence so that this sequence can be easily replaced false Chushu Zhu annotation2500134 1 RBS range2500134 1 1027 1056 annotation2500130 1 barcode range2500130 1 224 228 annotation2500135 1 FKBP range2500135 1 1057 1380 annotation2500132 1 10aa linker range2500132 1 700 729 annotation2500133 1 FRB range2500133 1 742 1026 annotation2500129 1 BBa_K1789012 range2500129 1 1 223 annotation2500136 1 10aa linker range2500136 1 1393 1422 annotation2500137 1 sGFP-C range2500137 1 1423 1668 annotation2500131 1 sGFP-N range2500131 1 229 699 annotation2500139 1 barcode range2500139 1 1669 1674 annotation2500140 1 BBa_B0015 range2500140 1 1675 1803 BBa_K1997017_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaaactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaggtggttctggtggtggttctggtggttctataggcgagaccatgatcctctggcatgagatgtggcatgaaggcctggaagaggcatctcgtttgtactttggggaaaggaacgtgaaaggcatgtttgaggtgctggagcccttgcatgctatgatggaacggggcccccagactctgaaggaaacatcctttaatcaggcctatggtcgagatttaatggaggcccaagagtggtgcaggaagtacatgaaatcagggaatgtcaaggacctcacccaagcctgggacctctattatcatgtgttccgacgaatctcatgataaacctaactaagtaattaaaggaaacagacgatgggagtgcaggtggaaaccatctccccaggagacgggcgcaccttccccaagcgcggccagacctgcgtggtgcactacaccgggatgcttgaagatggaaagaaatttgattcctcccgggacagaaacaagccctttaagtttatgctaggcaagcaggaggtgatccgaggctgggaagaaggggttgcccagatgagtgtgggtcagagagccaaactgactatatctccagattatgcctatggtgccactgggcacccaggcatcatcccaccacatgccactctcgtcttcgatgtggagcttctaaaactggaaggtctcagtttaggttctggtggtggttctggtggtggttctaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataaactagaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z