LOCUS       BBa_K1997022            2451 bp    DNA     linear       30-Dec-2025
DEFINITION  P+R->sLuc-N->Zif-N->RBS->Zif-C->sLuc-C->TER
ACCESSION   BBa_K1997022
VERSION     BBa_K1997022.1  
FEATURES             Location/Qualifiers
     misc_feature    1..223
                     /label=	BBa_K1789012
     misc_feature    224..228
                     /label=	barcode
     CDS             229..1476
                     /label=	sLUC-N
     CDS             1477..1506
                     /label=	10aa linker
     CDS             1519..1707
                     /label=	Zif-N
     RBS             1708..1737
                     /label=RBS
     CDS             1738..1815
                     /label=	Zif-C
     CDS             1828..1857
                     /label=	10aa linker
     CDS             1858..2316
                     /label=	sLUC-C
     misc_feature    2317..2322
                     /label=	barcode
     misc_feature    2323..2451
                     /label=	BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagat taaagaggag aaaactagat ggaagacgcc
      241 aaaaacataa agaaaggccc ggcgccattc tatccgctgg aagatggaac cgctggagag
      301 caactgcata aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat
      361 gcacatatcg aggtgaacat cacgtacgcg gaatacttcg aaatgtccgt tcggttggca
      421 gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac
      481 tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc
      541 gcgaacgaca tctataacga acgcgagttg ttaaactcta tgaacatctc ccaacctact
      601 gtagtttttg tctcgaagaa aggtctgcaa aaaattctga atgtccagaa gaagctgccc
      661 attattcaga agatcatcat catggactct aaaacggatt accagggatt tcagtcgatg
      721 tacacgttcg tcacgtctca tctacctccc ggttttaatg aatacgattt tgtaccagag
      781 tcctttgatc gtgacaaaac aattgcactg ataatgaact cctctgggtc tactgggtta
      841 cctaagggtg tggcccttcc gcatagaact gcctgcgtca gattctcgca tgccagagat
      901 cctatttttg gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat
      961 cacggttttg gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtcctt
     1021 atgtaccgtt ttgaagagga gctgttcctt cgttcgttgc aggactataa aattcaatct
     1081 gcactgcttg tcccaacttt gttctcgttt ttcgcaaagt ccacattgat cgacaaatat
     1141 gacttatcta acttgcacga aattgcttct gggggcgcac ctctttcgaa agaagtcggg
     1201 gaagcggttg caaaacgctt ccatcttcca gggatacgac aaggatatgg gctcactgag
     1261 actacatcag ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa
     1321 gttgttccat tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt
     1381 aatcagagag gcgaattatg tgtcagagga cctatgatta tgtccggcta tgtcaacaac
     1441 cccgaagcca cgaatgccct gattgacaag gacggaggtg gttctggtgg tggttctggt
     1501 ggttctatag gcgagacccc gggtgaaaaa ccgtacgctt gcccggttga atcttgcgac
     1561 cgtcgtttct ctcgttctga cgaactgacc cgtcacatcc gtatccacac cggtcagaaa
     1621 ccgttccagt gccgtatctg catgcgtaac ttctctcgtt ctgaccacct gaccacccac
     1681 atccgtaccc acaccggtga aaaataaacc taactaagta attaaaggaa acagacgatg
     1741 ccgttcgctt gcgacatctg cggtcgtaaa ttcgctcgtt ctgacgaacg taaacgtcac
     1801 accaaaatcc acaccggtct cagtttaggt tctggtggtg gttctggtgg tggttcttcc
     1861 ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat
     1921 tctggagaca tagcttactg ggacgaagac gaacacttct tcatagttga ccgcttgaag
     1981 tctttaatta aatacaaagg atatcaggtg gcccccgctg aattggaatc gatattgtta
     2041 caacacccca acatcttcga cgcgggcgtg gcaggtcttc ccgacgatga cgccggtgaa
     2101 cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg
     2161 gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg
     2221 gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc
     2281 ataaaggcca agaagggcgg aaagtccaaa ttgtaaacta gaccaggcat caaataaaac
     2341 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     2401 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
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