LOCUS BBa_K1997024 5180 bp DNA linear 09-Jun-2024 DEFINITION P+R->sHRP-N->dCas9->Ter ACCESSION BBa_K1997024 VERSION BBa_K1997024.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..223 /label=RBS30 RBS 209..223 /label=BBa_K1789002 misc_feature 1..223 /label=BBa_K1789012 CDS 232..261 /label=10aa linker CDS 262..903 /label=sHRP-N CDS 904..933 /label=10aa linker CDS 232..933 /label=BBa_K1997000 CDS 940..5043 /label=dCas9 CDS 940..5043 /label=BBa_K1997010 stem_loop 5063..5106 /label=stem_loop misc_feature 5052..5131 /label=BBa_B0010 terminator 5052..5131 /label=BBa_B0010 stem_loop 5147..5166 /label=T7 TE misc_feature 5173..5173 /label=stop polyA_site 5167..5180 /label=polya misc_feature 5140..5180 /label=BBa_B0012 terminator 5140..5180 /label=BBa_B0012 terminator 5052..5180 /label=BBa_B0015 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga gaaaggttct 241 ggttctacct ctggttctgg tatgcagtta acccctacat tctacgacaa tagctgtccc 301 aacgtgtcca acatcgttcg cgacattatc gtcaacgagc tcagatccga tcccaggatc 361 gctgcttcaa tattacgtct gcacttccat gactgcttcg tgaatggttg cgacgctagc 421 atattactgg acaacaccac cagtttccgc actgaaaagg atgcattcgg gaacgctaac 481 agcgccaggg gctttagcgt gatcgatcgc atgaaggctg ccgttgagtc agcatgccca 541 ggtacagtca gttgtgcaga cctgctgact atagctgcgc aacagagcgt gactcttgca 601 ggcggaccgt cctggagagt gccgctcggt cgacgtgact ccctacaggc attcctagat 661 ctggccaacg ccaacttgcc tgctccattc ttcaccctgc cccagctgaa ggatagcttt 721 agaaacgtgg gtctgaatcg ctcaagtgac cttgtggctc tgtccggagg acacacattt 781 ggaaagagcc agtgtaggtt catcatggat aggctctaca atttcagcaa cactgggtta 841 cctgacccca cgctgaacac tacgtatctc cagacactga gaggcttgtg cccactgaat 901 ggcaaaggtt ctggttctac ctctggttct ggttactaga tggataagaa atactcaata 961 ggcttagcta tcggcacaaa tagcgtcgga tgggcggtga tcactgatga atataaggtt 1021 ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc acagtatcaa aaaaaatctt 1081 ataggggctc ttttatttga cagtggagag acagcggaag cgactcgtct caaacggaca 1141 gctcgtagaa ggtatacacg tcggaagaat cgtatttgtt atctacagga gattttttca 1201 aatgagatgg cgaaagtaga tgatagtttc tttcatcgac ttgaagagtc ttttttggtg 1261 gaagaagaca agaagcatga acgtcatcct atttttggaa atatagtaga tgaagttgct 1321 tatcatgaga aatatccaac tatctatcat ctgcgaaaaa aattggtaga ttctactgat 1381 aaagcggatt tgcgcttaat ctatttggcc ttagcgcata tgattaagtt tcgtggtcat 1441 tttttgattg agggagattt aaatcctgat aatagtgatg tggacaaact atttatccag 1501 ttggtacaaa cctacaatca attatttgaa gaaaacccta ttaacgcaag tggagtagat 1561 gctaaagcga ttctttctgc acgattgagt aaatcaagac gattagaaaa tctcattgct 1621 cagctccccg gtgagaagaa aaatggctta tttgggaatc tcattgcttt gtcattgggt 1681 ttgaccccta attttaaatc aaattttgat ttggcagaag atgctaaatt acagctttca 1741 aaagatactt acgatgatga tttagataat ttattggcgc aaattggaga tcaatatgct 1801 gatttgtttt tggcagctaa gaatttatca gatgctattt tactttcaga tatcctaaga 1861 gtaaatactg aaataactaa ggctccccta tcagcttcaa tgattaaacg ctacgatgaa 1921 catcatcaag acttgactct tttaaaagct ttagttcgac aacaacttcc agaaaagtat 1981 aaagaaatct tttttgatca atcaaaaaac ggatatgcag gttatattga tgggggagct 2041 agccaagaag aattttataa atttatcaaa ccaattttag aaaaaatgga tggtactgag 2101 gaattattgg tgaaactaaa tcgtgaagat ttgctgcgca agcaacggac ctttgacaac 2161 ggctctattc cccatcaaat tcacttgggt gagctgcatg ctattttgag aagacaagaa 2221 gacttttatc catttttaaa agacaatcgt gagaagattg aaaaaatctt gacttttcga 2281 cttccttatt atgttggtcc attggcgcgt ggcaatagtc gttttgcatg gatgactcgg 2341 aagtctgaag aaacaattac cccatggaat tttgaagaag ttgtcgataa aggtgcttca 2401 gctcaatcat ttattgaacg catgacaaac tttgataaaa atcttccaaa tgaaaaagta 2461 ctaccaaaac atagtttgct ttatgagtat tttacggttt ataacgaatt gacaaaggtc 2521 aaatatgtta ctgaaggaat gcgaaaacca gcatttcttt caggtgaaca gaagaaagcc 2581 attgttgatt tactcttcaa aacaaatcga aaagtaaccg ttaagcaatt aaaagaagat 2641 tatttcaaaa aaatagaatg ttttgatagt gttgaaattt caggagttga agatagattt 2701 aatgcttcat taggtaccta ccatgatttg ctaaaaatta ttaaagataa agattttttg 2761 gataatgaag aaaatgaaga tatcttagag gatattgttt taacattgac cttatttgaa 2821 gatagggaga tgattgagga aagacttaaa acatatgctc acctctttga tgataaggtg 2881 atgaaacagc ttaaacgtcg ccgttatact ggttggggac gtttgtctcg aaaattgatt 2941 aatggtatta gggataagca atctggcaaa acaatattag attttttgaa atcagatggt 3001 tttgccaatc gcaattttat gcagctgatc catgatgata gtttgacatt taaagaagac 3061 attcaaaaag cacaagtgtc tggacaaggc gatagtttac atgaacatat tgcaaattta 3121 gctggtagcc ctgctattaa aaaaggtatt ttacagactg taaaagttgt tgatgaattg 3181 gtcaaagtaa tggggcggca taagccagaa aatatcgtta ttgaaatggc acgtgaaaat 3241 cagacaactc aaaagggcca gaaaaattcg cgagagcgta tgaaacgaat cgaagaaggt 3301 atcaaagaat taggaagtca gattcttaaa gagcatcctg ttgaaaatac tcaattgcaa 3361 aatgaaaagc tctatctcta ttatctccaa aatggaagag acatgtatgt ggaccaagaa 3421 ttagatatta atcgtttaag tgattatgat gtcgatgcca ttgttccaca aagtttcctt 3481 aaagacgatt caatagacaa taaggtctta acgcgttctg ataaaaatcg tggtaaatcg 3541 gataacgttc caagtgaaga agtagtcaaa aagatgaaaa actattggag acaacttcta 3601 aacgccaagt taatcactca acgtaagttt gataatttaa cgaaagctga acgtggaggt 3661 ttgagtgaac ttgataaagc tggttttatc aaacgccaat tggttgaaac tcgccaaatc 3721 actaagcatg tggcacaaat tttggatagt cgcatgaata ctaaatacga tgaaaatgat 3781 aaacttattc gagaggttaa agtgattacc ttaaaatcta aattagtttc tgacttccga 3841 aaagatttcc aattctataa agtacgtgag attaacaatt accatcatgc ccatgatgcg 3901 tatctaaatg ccgtcgttgg aactgctttg attaagaaat atccaaaact tgaatcggag 3961 tttgtctatg gtgattataa agtttatgat gttcgtaaaa tgattgctaa gtctgagcaa 4021 gaaataggca aagcaaccgc aaaatatttc ttttactcta atatcatgaa cttcttcaaa 4081 acagaaatta cacttgcaaa tggagagatt cgcaaacgcc ctctaatcga aactaatggg 4141 gaaactggag aaattgtctg ggataaaggg cgagattttg ccacagtgcg caaagtattg 4201 tccatgcccc aagtcaatat tgtcaagaaa acagaagtac agacaggcgg attctccaag 4261 gagtcaattt taccaaaaag aaattcggac aagcttattg ctcgtaaaaa agactgggat 4321 ccaaaaaaat atggtggttt tgatagtcca acggtagctt attcagtcct agtggttgct 4381 aaggtggaaa aagggaaatc gaagaagtta aaatccgtta aagagttact agggatcaca 4441 attatggaaa gaagttcctt tgaaaaaaat ccgattgact ttttagaagc taaaggatat 4501 aaggaagtta aaaaagactt aatcattaaa ctacctaaat atagtctttt tgagttagaa 4561 aacggtcgta aacggatgct ggctagtgcc ggagaattac aaaaaggaaa tgagctggct 4621 ctgccaagca aatatgtgaa ttttttatat ttagctagtc attatgaaaa gttgaagggt 4681 agtccagaag ataacgaaca aaaacaattg tttgtggagc agcataagca ttatttagat 4741 gagattattg agcaaatcag tgaattttct aagcgtgtta ttttagcaga tgccaattta 4801 gataaagttc ttagtgcata taacaaacat agagacaaac caatacgtga acaagcagaa 4861 aatattattc atttatttac gttgacgaat cttggagctc ccgctgcttt taaatatttt 4921 gatacaacaa ttgatcgtaa acgatatacg tctacaaaag aagttttaga tgccactctt 4981 atccatcaat ccatcactgg tctttatgaa acacgcattg atttgagtca gctaggaggt 5041 gactactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 5101 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 5161 tgggcctttc tgcgtttata //